Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(<scp>iii</scp>)-oxidizing bacterium: implications for arsenic immobilization

Lopez-Adams, Rebeca; Fairclough, Simon M.; Lyon, I. C.; Haigh, Sarah J.; Zhang, Jun; Zhao, Fang‐Jie; Moore, Katie L.; Lloyd, Jonathan R.

doi:10.1039/d1en00905b

Cited by 7 publications

(4 citation statements)

References 79 publications

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“…Also, a microbial scenario of its formation, including the so-called forced biomineralization [ 70 ], is well documented in the literature. Bacterial biomineralization of lepidocrocite has been reported for diverse nitrate-reducing Fe(II)-oxidizing bacteria [ 71 ], as well as in denitrifying As(III)-oxidizing bacterium [ 72 ] under anaerobic conditions. Also, it was observed that iron oxyhydroxide crystallization could be directed during the cultivation of Leptothrix sp.…”

Section: Discussionmentioning

confidence: 99%

Spongin as a Unique 3D Template for the Development of Functional Iron-Based Composites Using Biomimetic Approach In Vitro

Kubiak,

Pajewska-Szmyt,

Kotula

et al. 2023

Marine Drugs

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Marine sponges of the subclass Keratosa originated on our planet about 900 million years ago and represent evolutionarily ancient and hierarchically structured biological materials. One of them, proteinaceous spongin, is responsible for the formation of 3D structured fibrous skeletons and remains enigmatic with complex chemistry. The objective of this study was to investigate the interaction of spongin with iron ions in a marine environment due to biocorrosion, leading to the occurrence of lepidocrocite. For this purpose, a biomimetic approach for the development of a new lepidocrocite-containing 3D spongin scaffold under laboratory conditions at 24 °C using artificial seawater and iron is described for the first time. This method helps to obtain a new composite as “Iron-Spongin”, which was characterized by infrared spectroscopy and thermogravimetry. Furthermore, sophisticated techniques such as X-ray fluorescence, microscope technique, and X-Ray diffraction were used to determine the structure. This research proposed a corresponding mechanism of lepidocrocite formation, which may be connected with the spongin amino acids functional groups. Moreover, the potential application of the biocomposite as an electrochemical dopamine sensor is proposed. The conducted research not only shows the mechanism or sensor properties of “Iron-spongin” but also opens the door to other applications of these multifunctional materials.

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Section: Discussionmentioning

confidence: 99%

Spongin as a Unique 3D Template for the Development of Functional Iron-Based Composites Using Biomimetic Approach In Vitro

Kubiak,

Pajewska-Szmyt,

Kotula

et al. 2023

Marine Drugs

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“…strain 2002 and Pseudomonas stutzeri LS-2), where cells were encrusted by forming Fe(III) minerals, leading to an incomplete reduction of nitrate [ 54 , 55 , 56 ]. In addition, the observed decrease in As(V) reduction in the presence of Fe(II) was also caused by the adsorption or co-precipitation with the formed Fe oxides, supported by the decrease of dissolved total As concentrations during incubation ( Figure S8 ) [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Arsenic Mobilization and Transformation by Ammonium-Generating Bacteria Isolated from High Arsenic Groundwater in Hetao Plain, China

Jiang

Shen

Shi

et al. 2022

IJERPH

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Arsenic (As) mobilization in groundwater involves biogeochemical cycles of carbon, iron, and sulfur. However, few studies have focused on the role of nitrogen-metabolizing bacteria in As mobilization, as well as in the transformation between inorganic and organic As in groundwater. In this study, the nitrogen and As metabolisms of Citrobacter sp. G-C1 and Paraclostridium sp. G-11, isolated from high As groundwater in Hetao Plain, China, were characterized by culture experiments and genome sequencing. The results showed Citrobacter sp. G-C1 was a dissimilatory nitrate-reducing bacterium. The dissimilatory nitrate reduction to ammonia (DNRA) and As-detoxifying pathways identified in the genome enabled Citrobacter sp. G-C1 to simultaneously reduce As(V) during DNRA. Paraclostridium sp. G-11 was a nitrogen-fixing bacterium and its nitrogen-fixing activity was constrained by As. Nitrogen fixation and the As-detoxifying pathways identified in its genome conferred the capability of As(V) reduction during nitrogen fixation. Under anaerobic conditions, Citrobacter sp. G-C1 was able to demethylate organic As and Paraclostridium sp. G-11 performed As(III) methylation with the arsM gene. Collectively, these results not only evidenced that ammonium-generating bacteria with the ars operon were able to transform As(V) to more mobile As(III) during nitrogen-metabolizing processes, but also involved the transformation between inorganic and organic As in groundwater.

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“…Arsenic has a very low background in biological systems, which makes it possible to use NanoSIMS to map the distribution of arsenic-containing molecules in cells and tissues with high resolution. 21,22 Correlative imaging methods have become important in mapping the chemical information from Nano-SIMS to the ultrastructural information from electron microscopy [i.e., transmission electron microscopy images or backscattered electron (BSE) images from a scanning electron microscope]. 20 Recently, subcellular distributions of drugs that contain platinum, 23−25 gold, 26,27 ruthenium, 28 bromine, 29−31 and iodine 32 have been mapped by NanoSIMS analysis in a label-free fashion, which demonstrated the capabilities of NanoSIMS analysis for investigating therapeutic subcellular partitioning.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The NanoSIMS instrument bombards cells with a 50 nm 133 Cs + beam, releasing negatively charged secondary ions that are collected, quantified, and used to create high-resolution images based solely on the elemental content of cells. , NanoSIMS analysis makes it possible to visualize (and quantify) therapeutic agents in biological systems without fluorescent labeling or with a minimum of elemental and isotopic labeling. Arsenic has a very low background in biological systems, which makes it possible to use NanoSIMS to map the distribution of arsenic-containing molecules in cells and tissues with high resolution. , Correlative imaging methods have become important in mapping the chemical information from NanoSIMS to the ultrastructural information from electron microscopy [ i.e. , transmission electron microscopy images or backscattered electron (BSE) images from a scanning electron microscope] .…”

Section: Introductionmentioning

confidence: 99%

Subcellular Partitioning of Arsenic Trioxide Revealed by Label-Free Imaging

et al. 2022

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Subcellular partitioning of therapeutic agents is highly relevant to their interactions with target molecules and drug efficacy, but studying subcellular partitioning is an enormous challenge. Here, we describe the application of nanoscale secondary ion mass spectrometry (NanoSIMS) analysis to define the subcellular pharmacokinetics of a cytotoxic chemotherapy drug, arsenic trioxide (ATO). We reasoned that defining the partitioning of ATO would yield valuable insights into the mechanisms underlying ATO efficacy. NanoSIMS imaging made it possible to define the intracellular fate of ATO in a label-free mannerand with high resolution and high sensitivity. Our studies of ATO-treated cells revealed that arsenic accumulates in the nucleolus. After prolonged ATO exposure, ∼40 nm arsenic- and sulfur-rich protein aggregates appeared in the cell nucleolus, nucleus, and membrane-free compartments in the cytoplasm, and our studies suggested that the partitioning of nanoscale aggregates could be relevant to cell survival. All-trans retinoic acid increased intracellular ATO levels and accelerated the nanoscale aggregate formation in the nucleolus. This study yielded fresh insights into the subcellular pharmacokinetics of an important cancer therapeutic agent and the potential impact of drug partitioning and pharmacokinetics on drug activity.

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Elucidating heterogeneous iron biomineralization patterns in a denitrifying As(iii)-oxidizing bacterium: implications for arsenic immobilization

Abstract: Anaerobic nitrate-dependent iron(ii) oxidation is a process common to many bacterial species, which promotes the formation of Fe(iii) minerals that can influence the fate of soil and groundwater pollutants, such as arsenic.

Cited by 7 publications

References 79 publications

Spongin as a Unique 3D Template for the Development of Functional Iron-Based Composites Using Biomimetic Approach In Vitro

Spongin as a Unique 3D Template for the Development of Functional Iron-Based Composites Using Biomimetic Approach In Vitro

Arsenic Mobilization and Transformation by Ammonium-Generating Bacteria Isolated from High Arsenic Groundwater in Hetao Plain, China

Subcellular Partitioning of Arsenic Trioxide Revealed by Label-Free Imaging

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