2020
DOI: 10.3390/vaccines8040660
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Elucidating the Efficacy of Vaccination against Vibriosis in Lates calcarifer Using Two Recombinant Protein Vaccines Containing the Outer Membrane Protein K (r-OmpK) of Vibrio alginolyticus and the DNA Chaperone J (r-DnaJ) of Vibrio harveyi

Abstract: Recombinant cell vaccines expressing the OmpK and DnaJ of Vibrio were developed and subsequently, a vaccination efficacy trial was carried out on juvenile seabass (~5 cm; ~20 g). The fish were divided into 5 groups of 50 fish per group, kept in triplicate. Groups 1 and 2 were injected with 107 CFU/mL of the inactivated recombinant cells vaccines, the pET-32/LIC-OmpK and pET-32/LIC-DnaJ, respectively. Group 3 was similarly injected with 107 CFU/mL of inactivated E. coli BL21 (DE3), Group 4 with 107 CFU/mL of fo… Show more

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Cited by 24 publications
(16 citation statements)
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“…The way these cytokines contribute to fish resistance to bacterial infection is yet unclear, but their expression during infection highlight their central role in driving a protective immune response. A higher expression of IL-1β was reported in the head kidney, spleen and intestine of Asian Seabass ( Lates calcarifer ) vaccinated with outer membrane protein K of Vibrio harveyi [27] . In rainbow trout ( Oncorhynchus mykiss ), a higher expression of IL-1β was found in the spleen and gills after immunization with a formalin-killed Yersinia ruckeri vaccine [28] and also in head kidney and peritoneal cells after vaccination with a formalin-killed bacterin of Aeromonas salmonicida [16] .…”
Section: Discussionmentioning
confidence: 96%
“…The way these cytokines contribute to fish resistance to bacterial infection is yet unclear, but their expression during infection highlight their central role in driving a protective immune response. A higher expression of IL-1β was reported in the head kidney, spleen and intestine of Asian Seabass ( Lates calcarifer ) vaccinated with outer membrane protein K of Vibrio harveyi [27] . In rainbow trout ( Oncorhynchus mykiss ), a higher expression of IL-1β was found in the spleen and gills after immunization with a formalin-killed Yersinia ruckeri vaccine [28] and also in head kidney and peritoneal cells after vaccination with a formalin-killed bacterin of Aeromonas salmonicida [16] .…”
Section: Discussionmentioning
confidence: 96%
“…Although other antigens have been pointed out as vaccine candidates, like TolC, an outer membrane protein from V. harveyi ( 85 ) and DNAj ( 86 ), several experimental vaccines using OmpK were described as efficient. For instance, vaccination of Orange-spotted groupers with a recombinant OmpK induced a higher tolerance to Vibrio infections ( 83 ); a subunit vaccine involving OmpK and OmpU fusion resulted in a protective effect against V. harveyi infection and showed potential against other species ( 87 ); E. coli based recombinant vaccines containing OmpK were efficient against challenge with multiple strains of V. harveyi , V. alginolyticus , and V. parahaemolyticus ( 86 ). Nonetheless, studies regarding either the immunogenicity of OmpK or the cross-protection effect of recombinant OmpK vaccines are not consistent.…”
Section: Discussionmentioning
confidence: 99%
“…Hence, it is now realized that these bacteria pose a considerable threat to tilapia farming, so producing an effective vaccine is rather urgent. In Malaysia, several types of vaccines, such as inactivated, recombinant, and live attenuated vaccines; Different routes of vaccination, such as oral, injectable, or immersion, were extensively explored by local researchers against S. agalactiae and S. iniae (Table -4) [41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59].…”
Section: Streptococcosis Vaccinementioning
confidence: 99%
“…• Fish vaccinated with r-OmpK had a 90% survival rate against V. harveyi and 100% against V. alginolyticus and Vibrio parahaemolyticus • The blood and gut lavage antibody of fish vaccinated with r-OmpK were increased significantly and TLR2, MyD88, and MHCI genes were upregulation in the kidney and intestinal tissues of r-OmpK vaccinated fish [59] S. iniae=Streptococcus iniae, A. hydrophila=Aeromonas hydrophila, V. harveyi=Vibrio harveyi, A. hydrophila=Aeromonas hydrophila, S. agalactiae=Streptococcus agalactiae, V. alginolyticus=Vibrio alginolyticus, RPS=Relative percent survival the feed-based vaccine (without adjuvant) and control group, respectively. Another laboratory trial of a feed-based vaccine against S. agalactiae in red tilapia showed that a double booster vaccination regime (weeks 0, 2, and 6) at 4% of body weight successfully provided a consistently high level of serum IgM antibody for 4 months [42].…”
Section: Intra-peritoneal Lab Trialmentioning
confidence: 99%
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