Elucidating the functional role of endoreduplication in tomato fruit development

Abstract

The cyclin-dependent kinase inhibitory kinase WEE1 and the anaphase promoting complex activator CCS52A both participate in the control of cell size and the endoreduplication process driving cell expansion during early fruit development in tomato. Moreover the fruit-specific functional analysis of the tomato CDK inhibitor KRP1 reveals that cell size and fruit size determination can be uncoupled from DNA ploidy levels, indicating that endoreduplication acts rather as a limiting factor for cell growth. The overall functional data contribute to unravelling the physiological role of endoreduplication in growth induction of fleshy fruits.

Endoreduplication in floral structure, vegetative and fruits of red pitaya with white pulp

“…Probably, the endoreduplication was selected during the evolution for the benefit of the plant and organ development (CHEVALIER et al, 2011). This phenomenon is important for the development process such as cell maintenance, response to physiological conditions like pest attack and on DNA damage (DE VEYLDER et al, 2011).…”

“…In plants, the occurrence of endoreduplication is common and documented in the endosperm of the cereal crops (SABELLI et al, 2009), cotton fiber (Gossypium hirsutum) (WILKINS et al, 2000), tomato fruits (CHEVALIER et al, 2011), nitrogen fixing legumes (KONDOROSI; KONDOROSI, 2004). It is also reported in the endosperm and cotyledon of the seeds in development (LEE et al, 2009).…”

“…It is known that during the development of some plants the normal cellular cycle can be substituted by the altered cellular cycle where did not occur mitoses (CHEVALIER et al, 2011). This altered cellular cycle is denominated as endocycle or endoreduplication which occur the DNA duplication without separation of chromosomes and cytokinesis (de VEYLDER et al, 2011).…”

Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato

“…After fruit set, early growing tomato fruit undergoes a phase of cell division followed by a phase of cell expansion associated with a large increase in cell size and nucleus ploidy level (up to 512 C) due to endoreduplication (Cheniclet et al 2005). Data mining and functional analyses in planta of candidate cell cycle-related genes led to the identification of several key players involved in the control of cell division and in the shift from mitotic cycle to endocycle in tomato fruit (Chevalier et al 2011) and therefore in fruit size control. However, as demonstrated for the cell divisionrelated FW2.2 gene controlling the main fruit size QTL in domesticated tomato (Frary et al 2000) and for the fasciated locus controlling tomato carpel number (Cong et al 2008), forward genetics approach appears as the most powerful for the identification of new genes susceptible to regulate fruit growth in tomato fruit.…”

Occurrence and significance of polysomaty in species of Mimosa L.

“…Despite several studies, the mechanisms and signals that are necessary to change from a classic cell cycle to endoreduplication, and to initiate and maintain the endocycles, as well as the physiological significance of endoreduplication are not well known. It is not clear if endoreduplication is genetically programmed or a consequence of differentiation (Kondorosi & Kondorosi 2004;Chevalier et al 2011). Environmental factors such as light, nutrition, temperature and hormones may influence endoreduplication and the degree of endopolyploidization and should be further studied (Barow 2006;Jovtchev et al 2007;Chen et al 2011).…”