Elucidating the interaction of rhizosphere bacteria and environmental factors in influencing active ingredient content of Lycium barbarum fruit in China

Abstract

Aims This study aimed to compare the differences in the bacterial community structure of Lycium barbarum rhizosphere and elucidate the contribution of rhizosphere bacteria to the active ingredients of L. barbarum fruit. Methods and Results This study investigated the soil and meteorological characteristics of L. barbarum rhizosphere during three growth stages across three production regions of China. High‐throughput sequencing showed significant differences in the bacterial community diversity of L. barbarum rhizosphere across the three production regions, and norank_o_Gaiellales, norank_f_Anaerolineaceae and norank_f_AKYG1722 were the highest in Ningxia. In addition, regression and path analysis revealed that pH, norank_o_Gaiellales and norank_f_AKYG1722 significantly promoted the accumulation of total sugar and flavonoids in L. barbarum fruit directly or indirectly. Soil organic matter (SOM), norank_f_Anaerolineaceae and humidity significantly promoted the accumulation of betaine. The average temperature during the growth stages, norank_f_AKYG1722, and norank_o_Gaiellales promoted the accumulation of polysaccharides. Conclusions The interaction between rhizosphere bacteria and environmental factors promoted the accumulation of active ingredients in L. barbarum fruits. Significance and Impact of the Study Our results provided insights to improve the quality of L. barbarum fruit.

Rhizosphere microbial markers (micro-markers): A new physical examination indicator for traditional Chinese medicines

“…In the stage of a certain medicinal plant susceptible to diseases and pests or the harvesting period, five-point sampling method ( Liu et al, 2022 , Liu et al, 2022 , Xiao et al, 2017 ) is used to collect the complete root system of the characteristic medicinal plant; if it is a tree, the hairy roots about 1 m away from the tree in four directions can be harvested, and the depth is about 10–30 cm ( Xu et al, 2018 ). The loosely bound non-rhizosphere soil was shaken off, and the roots with soil were eluted thrice with PBS for 15 s of each time.…”

“…Sun et al (2023) found that the relative abundances of Vicinamibacterales and Rhizobiales were significantly correlated with wogonoside, the active ingredient of Scutellaria baicalensis Georgi. The rhizosphere microorganisms norank_f_Anaerolineaceae in Lycium barbarum L. can promote the accumulation of betaine, while norank_f_AKYG1722 and norank_o_Gaiellales promoted the accumulation of polysaccharide ( Liu et al, 2022 , Liu et al, 2022 ). Alternaria is closely related to paeoniflorin accumulation in Paeonia lactiflora Pall.…”

Chemical and Biological Properties of Three Poorly Studied Species of Lycium Genus—Short Review

“…Wolfberries are also a source of dietary fibers, some minerals (K, Cu, Mn, Fe, Zn), fatty acids (linoleic, oleic, palmitic, and stearic acids), amino acids (e.g., proline and serine) and non-protein amino acids (e.g., γ-aminobutyric acid, hydroxyproline, and citrulline) [43]. The chemical profile of these berries is dependent on diverse factors, such as environmental conditions, pre-and post-harvest factors, rhizosphere bacterial community structure and genetic heritage [43,44].…”