Elucidating the Probiotic Potential of Malaysian Paenibacillus pabuli Against Vibrio alginolyticus in Artemia Culture

Ina-Salwan, M.Y.; Hishammudd, H.; Zulperi, Zarirah; Salema, M.; Karim, Murni; Ikhsan, Natrah Fatin Mohd

doi:10.3923/ajar.2015.223.236

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Meanwhile, Gram-negative Paenibacillus, a member of the Firmicutes phylum, has been found in a variety of environmental studies, including animals and fish, palm oil waste, and plants. Interestingly, only a few species of the Paenibacillus genus have been isolated from forest soil, especially in the tropical regions (Ina-Salwany et al 2015;Grady et al 2016;Chin et al 2017;Haruna et al 2017;Huang et al 2017;Kim et al 2021). Gram-positive Staphylococcus on the other hand is likely to be found in an animal's intestinal tract instead of the environmental samples.…”

Section: Resultsmentioning

confidence: 99%

Isolation, identification, and characterization of phosphate solubilizing bacteria, Paenibacillus sp., from the soil of Danum Valley Tropical Rainforest, Sabah, Malaysia

et al. 2021

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While phosphorus (P) is a vital element for the plant to grow, only 0.1% of the phosphate soil is directly to be uptake by plants. Consequently, P fertilizer, which is mostly taken from unrenewable resources of phosphate rock, is practically added into the croplands. Nevertheless, as the demand for this fertilizer kept increasing, the availability of resources and environmental issues are currently raising wide concerns. Alternatively, soil phosphate solubilizing bacteria (PSB) is promising to be further developed as a biofertilizer to increase the availability of P elements for plants. This study aims to screen and characterize novel PSB from the tropical rainforest soil. The soil samples were collected from the Danum Valley tropical rainforest, Sabah. Phosphatase solubilizing bacteria were then screened using the NBRIP Agar selective media. The screening results yielded five colonies, designated as PSB1, PSB2, PSB3, PSB4, and PSB5, displaying halos, with an average diameter of 10mm. Further, 16s rRNA gene sequence analysis using BLASTn suggested that PSB1, PSB2, PSB3, PSB4, and PSB5 were designated as Bacillus sp. PSB01, Pseudomonas oryzyhabitans PSB02, Staphylococcus pasteuri PSB03, Paenibacillus sp. PSB04, and Staphylococcus pasteuri PSB05, respectively. Interestingly, the Paenibacillus group is a promising biofertilizer and is currently used in the global agriculture industry. Accordingly, Paenibacillus sp. PSB04 was then selected for further characterization using Gram staining and observed under scanning electron microscope (SEM). The Gram staining revealed that Paenibacillus sp. PSB04 is a Gram-negative bacterium with a rod shape, which is in good agreement with the SEM result. The specific phosphatase activity of the extracellular fraction of this bacterium was 7378.12 U mg-1 which is the highest activity compared to previous studies. This study provides an early insight into an excellent phosphate solubilizing bacterium for the agriculture industry obtained from Danum Valley.

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Section: Resultsmentioning

confidence: 99%

Isolation, identification, and characterization of phosphate solubilizing bacteria, Paenibacillus sp., from the soil of Danum Valley Tropical Rainforest, Sabah, Malaysia

et al. 2021

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Strategies of Controlling Vibriosis in Fish

Huzmi

Yasin²,

Ikhsan³

et al. 2019

AJAS

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The growth of the aquaculture industry is becoming more important in the food production sector. Presently, the biggest problem in aquaculture is bacterial diseases that infect the host thus causing outbreaks and economic losses. Vibriosis is a potentially lethal threat in commercial fish farming. Based on previous researches, various infections of the Vibrio genus have been reported hence their virulence is not fully understood. This review aims to provide enlightenment through research findings on Vibriosis-infected fish. Besides, some guidelines regarding control measures of Vibrio in aquaculture, such as the application of immunostimulant, antibiotics, probiotics and quorum sensing molecules, will be also reviewed.

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Species Identification of Potential Probiotic Lactic Acid Bacteria Isolated from Malaysian Fermented Food Based on 16S Ribosomal RNA (16S rRNA) and Internal Transcribed Spacer (ITS) Sequences

Ilyanie,

Huda Faujan,

Ida Muryany

2023

MAB

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Taxonomic identification of potential probiotic lactic acid bacteria (LAB) in fermented foods is essential as the microorganisms’ benefits are varied and often strain-specific. Using biochemical and physiological methods alone is inadequate to precisely distinguish each strain. In this study, molecular techniques were employed in the identification of 17 LAB isolated from three Malaysian fermented foods: belacan (BE), bosou (BO), and budu (BUM). The respective sizes of polymerase chain reaction (PCR) products from the isolates were approximately 1500 bp and 750 bp when amplified with the 16S ribosomal RNA (16S rRNA) and internal transcribed spacer (ITS) gene primers. The phylogenetic analysis using both gene sequences revealed that all BE and BO isolates were identified as Lactiplantibacillus plantarum, while all BUM isolates were identified as Lacticaseibacillus paracasei. Both 16S rRNA and ITS genes could disclose the identity of the isolates up to the species level. In summary, the use of the ITS gene in conjunction with the 16S rRNA gene can help with the more effective identification of potential probiotic LAB strains isolated from fermented food.

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Elucidating the Probiotic Potential of Malaysian Paenibacillus pabuli Against Vibrio alginolyticus in Artemia Culture

Cited by 3 publications

References 32 publications

Isolation, identification, and characterization of phosphate solubilizing bacteria, Paenibacillus sp., from the soil of Danum Valley Tropical Rainforest, Sabah, Malaysia

Isolation, identification, and characterization of phosphate solubilizing bacteria, Paenibacillus sp., from the soil of Danum Valley Tropical Rainforest, Sabah, Malaysia

Strategies of Controlling Vibriosis in Fish

Species Identification of Potential Probiotic Lactic Acid Bacteria Isolated from Malaysian Fermented Food Based on 16S Ribosomal RNA (16S rRNA) and Internal Transcribed Spacer (ITS) Sequences

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