Elucidation of Genetic Backgrounds Necessary for Chlorophyll a Biosynthesis Toward Artificial Creation of Oxygenic Photosynthesis

Tsukatani, Yusuke; Masuda, Shinji

doi:10.1007/s11084-015-9446-1

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…Chlorophyll is an important tetrapyrrole pigment, which is responsible for harvesting and conversion of solar energy into its chemical form during the process of oxygenic photosynthesis on our planet (Gust et al, 2001;Nelson and Junge, 2015;Tsukatani and Masuda, 2015). Chlorophyll and different other pigments are produced as metabolic products (Reinbothe et al, 2010) of a complex biosynthetic pathway.…”

Section: Introductionmentioning

confidence: 99%

A Novel Structural and Functional Insight Into Chloroplast-Encoded Central Subunit of Dark-Operated Protochlorophyllide Oxidoreductase (Dpor) of Plants

Qamar¹

2017

PAKJAS

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Chlorophyll converts harvested light into chemical energy during the strategic process of photosynthesis in chloroplasts. Protochlorophyllide reduction in the chlorophyll formation is catalyzed by two complex enzymes; light-dependent protochlorophyllide oxidoreductase (LPOR) and dark-operated protochlorophyllide oxidoreductase (DPOR). Of these two, DPOR is a three-subunit complex in which ChlB plays a vital role in developing photosynthetically competent chloroplasts. What has not been reported before is the complete structural and functional annotation of ChlB subunit from plants. Sequence, structure and functional analyses of the ChlB subunit are performed using a blend of molecular biology and bioinformatics approaches to identify conserved residues and distribution of amino acids. Complete ChlB sequence analysis coupled with phylogenetic analysis, molecular docking and protein-protein interaction reveal that the ChlB is thermo-stable, acidic and hydrophilic in nature. The 3D structure (RMSD of 0.20Å) of ChlB is predicted and used as a target in docking and protein-protein interaction studies. Structural characterization of ChlB further elucidates that the amino acids Arg18, Asn175, Glu221 and Asp311 being important catalytic residues are involved in the basic function of ChlB and its interaction with other DPOR subunits. Further, the mutation analysis substantiates the central role of predicted catalytic residues in the structure of the ChlB. We conclude that the generated information will facilitate researchers in engineering chlorophyll pathway to improve photosynthesis in plants.

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Section: Introductionmentioning

confidence: 99%

A Novel Structural and Functional Insight Into Chloroplast-Encoded Central Subunit of Dark-Operated Protochlorophyllide Oxidoreductase (Dpor) of Plants

Qamar¹

2017

PAKJAS

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Investigation of Physiological and Biochemical Responses and Essential oil Yieldof Peppermint under Salt Stress

Gikloo

Mehrabi

Jahanbakhsh

et al. 2018

Biosci., Biotech. Res. Asia

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Peppermint (Menthapiperita L.) is aneconomically important medicinal and aromatic plant grown in different areas worldwide. Secondary metabolites were fundamentally produced by genetic processing;however, environmental factors affect their biosynthesis. Salinity is the most important abiotic stress which induces morphological, physiological, and biochemical changes in plants.To investigate the influence of salinity stress (0, 25, 50, 75, 100 and 125 mMNaCl)on chlorophyll content, stomatal conductance, relative water content (RWC), proline, Na+ and K+ content, antioxidant enzymes of catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO), protein, essential oil yield and dry weight of peppermint, a greenhouse study was conducted. The results indicated that salinity had a significant effect on foregoing parameters. Changes in chlorophyll content werepeak and stomata conductivity was a single function. Based on estimations, the highest chlorophyll contentwas recorded for low salinity (60 mMNaCl).The plant proline content was higher in stress condition compared tocontrol plants. The highest proline content observed in 125mM NaClconcentration was two times higher than that of control plants.There was an increase and then decrease in CAT and POX activities, respectively, in lower and sever levels of salinity.A single equation was the best-fit equation for changing PPO enzyme activity under stress conditions.The dry matter has been affected dramatically by salinity and decreased from 11.34g under the non-stress condition to 4.24 g under high stress condition. Essential oil percentage (in dry matter) increased in moderate salinity stress. We found that the amount of essential oil per plant was linearly decreased. So, the highest (9.78 g plant-1) amount of essential oil per plant belonged to control group and the lowest (4.6 g plant-1) wasobserved for full stress condition.

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Bacteriochlorophyll Fluorescence of Green Sulfur Bacteria in Anaerobic Zone of Two Natural Water Bodies

et al. 2018

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Elucidation of Genetic Backgrounds Necessary for Chlorophyll a Biosynthesis Toward Artificial Creation of Oxygenic Photosynthesis

Cited by 3 publications

References 5 publications

A Novel Structural and Functional Insight Into Chloroplast-Encoded Central Subunit of Dark-Operated Protochlorophyllide Oxidoreductase (Dpor) of Plants

A Novel Structural and Functional Insight Into Chloroplast-Encoded Central Subunit of Dark-Operated Protochlorophyllide Oxidoreductase (Dpor) of Plants

Investigation of Physiological and Biochemical Responses and Essential oil Yieldof Peppermint under Salt Stress

Bacteriochlorophyll Fluorescence of Green Sulfur Bacteria in Anaerobic Zone of Two Natural Water Bodies

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