Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila

Lin, Shanmeng; Kong, Tongtong; Ren, Xin; Li, Shengkang; Gong, Yi

doi:10.1007/s10126-020-09987-y

Cited by 11 publications

(7 citation statements)

References 49 publications

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“…Firmicutes represented 78% of analyzed sequences in the current study. Previous studies reported that other phyla, such as Proteobacteria , Acidobacteria , and Bacteroidetes , dominated the intestinal assemblage of Chinese mitten crabs (Shi et al 2019 ; Su et al 2020 ), fiddler crabs (Cuellar-Gempeler and Leibold 2018 , 2019 ), mud crabs (Wei et al 2019b ; Lin et al 2020 ), and vent crabs (Zhang et al 2017 ). We also found that Proteobacteria and Bacteroidetes dominated wild and pond-raised Chinese mitten crabs (Li et al 2007 ).…”

Section: Discussionmentioning

confidence: 99%

A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

Guan

Zhao

et al. 2021

AMB Expr

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The Chinese mitten crab (Eriocheir sinensis) is a popular aquaculture product in East Asia, especially in China. In the last decade, rice–crab co-culture has rapidly expanded in China. Under this model, crabs are raised in rice fields instead of in traditional aquaculture ponds. In this study, we cultured two varieties of Chinese mitten crabs (Changjiang and Liaohe) in an alkaline region in northwest China and used Illumina MiSeq sequencing to compare the intestinal bacterial alpha diversity and community structure between traditional and co-culture aquaculture models, between two crab varieties, and between female and male crabs. Significant variations in intestinal bacterial communities were found between crab varieties and between female and male crabs but not between aquaculture models. These results show that rice–crab co-culture operations did not obviously impact the crab intestinal bacterial community compared with traditional pond aquaculture. Firmicutes was the most abundant bacterial phylum in the crab intestines (78%, relative abundance). Three dominant operational taxonomic units (OTUs) represented 73.2% of Firmicutes sequences and 56.8% of all sequences. A dominant OTU assigned as Firmicutes that was negatively correlated with crab body length, width, and weight was found in the source water for the experimental area. The results of this study suggest that the aquaculture of Chinese mitten crabs in alkaline regions requires more study to improve cultivation techniques.

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Section: Discussionmentioning

confidence: 99%

A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

Guan

Zhao

et al. 2021

AMB Expr

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“…Based on our previous studies ( 55 , 56 ), the period 24 h postinfection was chosen for sampling in this study. Total RNA of hemolymph collected from mud crabs was isolated using TRIzol reagent (Ambion, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

p53 Ubiquitination Comediated by HUWE1 and TRAF6 Contributes to White Spot Syndrome Virus Infection in Crustacean

Gong

Kong

Aweya

et al. 2022

J Virol

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p53, the guardian of the genome, is a short-lived protein that is tightly controlled at low levels by constant ubiquitination and proteasomal degradation in higher organisms. p53 stabilization and activation are early crucial events to cope with external stimuli in cells. However, the role of p53 ubiquitination and its relevant molecular mechanisms have not been addressed in invertebrates. In this study, our findings revealed that both HUWE1 (HECT, UBA, and WWE domain-containing E3 ubiquitin-protein ligase 1) and TRAF6 (TNF receptor associated factor 6) could serve as E3 ubiquitin ligases for p53 in mud crab ( Scylla paramamosain ). Moreover, the expressions of HUWE1 and TRAF6 were significantly down-regulated during WSSV (White spot syndrome virus) infection, and therefore the ubiquitination of p53 was interrupted, leading to the activation of apoptosis and ROS (Reactive oxygen species) signals through p53 accumulation, which eventually suppressed viral invasion in mud crab. To the best of our knowledge, this is the first study to reveal the p53 ubiquitination simultaneously induced by two E3 ligases in arthropods, which provides a novel molecular mechanism of invertebrates in resistance to viral infection. Importance p53, which is a well-known tumor suppressor that has been widely studied in higher animals, has been reported to be tightly controlled at low levels by Ubiquitin-dependent proteasomal degradation. However, the recent p53 ubiquitination-relevant research is mainly involved in an individual E3 ubiquitin ligase, whether there exist other mechanisms that need to be explored. The results of this study showed that HUWE1 and TRAF6 could serve as p53 E3 ubiquitin ligases, and synchronously mediate p53 ubiquitination in mud crab, which confirmed the diversity of the p53 ubiquitination regulatory pathway. In addition, the effects of p53 ubiquitination are mainly focused on tumorigenesis, but few on the host immune defense in invertebrates. Our findings revealed that p53 ubiquitination could affect ROS and apoptosis signals to cope with WSSV infection in mud crab, which firstly clarified the immunologic functions and mechanisms of p53 ubiquitination in invertebrates.

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“…The V4 region of the bacterial 16S rRNA gene was amplified using the primers 515F (5 -GTGCCAGCMGCCGCGGTAA-3 ) and 806R (5 -GGACTACHVGGGTWTCTAAT-3 ) in PCR reactions. Each PCR reaction performed consisted of 10 ng of DNA templates, 2 µM of forward and reverse primers, and 15 µL of Phusion ® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA) [31]. Thermal cycling was carried out with an initial denaturation at 98 • C for 1 min, followed by 30 cycles of denaturation at 98 • C for 10 s, annealing at 50 • C for 30 s, and elongation at 72 • C for 30 s, with a final extension at 72 • C for 5 min.…”

Section: Dna Extraction Pcr Amplification Library Preparation and Seq...mentioning

confidence: 99%

“…Thermal cycling was carried out with an initial denaturation at 98 • C for 1 min, followed by 30 cycles of denaturation at 98 • C for 10 s, annealing at 50 • C for 30 s, and elongation at 72 • C for 30 s, with a final extension at 72 • C for 5 min. The PCR products were detected on a 2% agarose gel and purified using a Qiagen Gel Extraction Kit (Qiagen, Hilden, Germany) [31]. Sequencing libraries were generated using a TruSeq ® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA), and index codes were added.…”

Section: Dna Extraction Pcr Amplification Library Preparation and Seq...mentioning

confidence: 99%

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Changes in Hemolymph Microbiota of Chinese Mitten Crab (Eriocheir sinensis) in Response to Aeromonas hydrophila or Staphylococcus aureus Infection

Kong,

Fan,

Tran

2023

Animals

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The Chinese mitten crab (Eriocheir sinensis) has significant economic potential in both the Chinese domestic and global markets. The hemolymph microbiota is known to play a critical role in regulating physiological and biochemical functions in crustaceans. However, the study of the hemolymph microbiota of E. sinensis in response to infections has not been undertaken. In this study, changes in the composition and function of the hemolymph microbiota in E. sinensis infected with either Staphylococcus aureus (Sa) or Aeromonas hydrophila (Ah) were investigated using 16S rRNA sequencing, with a phosphate buffer saline (PBS) injection serving as the control. Results showed that the dominant hemolymph microbiota of E. sinensis were Proteobacteria, Bacteroidota, and Firmicutes. The relative abundance of the phyla Firmicutes, Bdellovibrionota, and Myxococcota was significantly reduced in both Sa and Ah groups compared to the PBS group. At the genus level, compared to the PBS group, a significant increase in the abundance of Flavobacterium and Aeromonas was found in both Ah and Sa groups. The analysis of the functional profile showed that pathways related to ‘cell growth and death’, ‘metabolism of terpenoids and polyketides’, ‘cancers’, ‘lipid metabolism’, ‘neurodegenerative diseases’, ‘metabolism of other amino acids’, ‘xenobiotics biodegradation and metabolism’, and ‘circulatory system and endocrine system’ were predominant in the Ah group. Meanwhile, pathways related to ‘metabolism or genetic information progressing’, such as ‘translation’, ‘metabolic diseases’, and ‘cellular processes and signaling’, were enriched in the Sa group. This study revealed the effects of pathogens (S. aureus or A. hydrophila) on the maintenance of the hemolymph microbiota in E. sinensis. It shed light on the mechanisms employed by the hemolymph microbiota of E. sinensis under pathogen stimulation.

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Elucidation of Gut Microbiota in Mud Crab Scylla paramamosain Challenged to WSSV and Aeromonas hydrophila

Cited by 11 publications

References 49 publications

A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

A high abundance of Firmicutes in the intestine of chinese mitten crabs (Eriocheir sinensis) cultured in an alkaline region

p53 Ubiquitination Comediated by HUWE1 and TRAF6 Contributes to White Spot Syndrome Virus Infection in Crustacean

Changes in Hemolymph Microbiota of Chinese Mitten Crab (Eriocheir sinensis) in Response to Aeromonas hydrophila or Staphylococcus aureus Infection

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