Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells

Bourhim, Thouria; Villareal, Myra O.; Gadhi, Chemseddoha; Isoda, Hiroko

doi:10.3390/nu13082697

Cited by 12 publications

(6 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results indicate that the whitening mechanisms of the tested cosmetic are complicated, 46 , 47 likely contributing to the inhibition of melanin expression and transport, as well as preventing pigmentation. Similar mechanisms have been inferred in previous cosmetics ingredient studies; for example, some natural ingredients, such as α-arbutin 48 and resveratrol 49 have been shown to decrease TYR activity to reduce melanin production, while Argan Press Cake (APC) 50 and nicotinamide 51 inhibited melanin transport.…”

Section: Resultssupporting

confidence: 77%

Epidermis-on-a-chip system to develop skin barrier and melanin mimicking model

Wang

et al. 2023

J Tissue Eng

View full text Add to dashboard Cite

In vitro skin models are rapidly developing and have been widely used in various fields as an alternative to traditional animal experiments. However, most traditional static skin models are constructed on Transwell plates without a dynamic three-dimensional (3D) culture microenvironment. Compared with native human and animal skin, such in vitro skin models are not completely biomimetic, especially regarding their thickness and permeability. Therefore, there is an urgent need to develop an automated biomimetic human microphysiological system (MPS), which can be used to construct in vitro skin models and improve bionic performance. In this work, we describe the development of a triple-well microfluidic-based epidermis-on-a-chip (EoC) system, possessing epidermis barrier and melanin-mimicking functions, as well as being semi-solid specimen friendly. The special design of our EoC system allows pasty and semi-solid substances to be effectively utilized in testing, as well as allowing for long-term culturing and imaging. The epidermis in this EoC system is well-differentiated, including basal, spinous, granular, and cornified layers with appropriate epidermis marker (e.g. keratin-10, keratin-14, involucrin, loricrin, and filaggrin) expression levels in corresponding layers. We further demonstrate that this organotypic chip can prevent permeation of over 99.83% of cascade blue (a 607 Da fluorescent molecule), and prednisone acetate (PA) was applied to test percutaneous penetration in the EoC. Finally, we tested the whitening effect of a cosmetic on the proposed EoC, thus demonstrating its efficacy. In summary, we developed a biomimetic EoC system for epidermis recreation, which could potentially serve as a useful tool for skin irritation, permeability, cosmetic evaluation, and drug safety tests.

show abstract

Section: Resultssupporting

confidence: 77%

Epidermis-on-a-chip system to develop skin barrier and melanin mimicking model

Wang

et al. 2023

J Tissue Eng

View full text Add to dashboard Cite

show abstract

“…Although argan seed cake has been used so far almost exclusively to feed animals [ 12 ], it has also been proposed as a natural exfoliant and moisturizer for use as an ingredient in commercial shampoo and in creams to topically treat sprains, or scabies, and for healing wounds. In particular, recent results suggest that argan seed cake has melanogenesis regulatory effects, and that its ethanol extract might be used as a component of natural whitening products and as pharmacological agent for the treatment of hyperpigmentation disorders [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Argan (Argania spinosa L.) Seed Oil Cake as a Potential Source of Protein-Based Film Matrix for Pharmaco-Cosmetic Applications

Mirpoor

Giosafatto

Mariniello

et al. 2022

IJMS

View full text Add to dashboard Cite

Various different agri-food biomasses might be turned into renewable sources for producing biodegradable and edible plastics, potentially attractive for food, agricultural and cosmeceutical sectors. In this regard, different seeds utilized for edible and non-edible oil extraction give rise to high amounts of organic by-products, known as seed oil cakes (SOCs), potentially able to become protein-rich resources useful for the manufacturing of biodegradable films. This study reports the potential of SOC derived from Argania spinosa (argan), a well-known plant containing valuable non-refined oil suitable for food or cosmetic use, to be a promising valuable source for production of a protein-based matrix of biomaterials to be used in the pharmaco-cosmetic sector. Thus, glycerol-plasticized films were prepared by casting and drying using different amounts of argan seed protein concentrate, in the presence of increasing glycerol concentrations, and characterized for their morphological, mechanical, barrier, and hydrophilicity properties. In addition, their antioxidant activity and effects on cell viability and wound healing were investigated. The hydrophobic nature of the argan protein-based films, and their satisfying physicochemical and biological properties, suggest a biorefinery approach for the recycling of argan SOC as valuable raw material for manufacturing new products to be used in the cosmeceutical and food industries.

show abstract

“…Even though argan seed cake has primarily been used for animal feed ( Cherki et al, 2006 ), it has also been suggested as a natural moisturizer and exfoliator for use in commercial shampoos and lotions to heal wounds, sprains, and scabies topically. Recent studies show that the ethanol extract of argan seed cake may be utilized as a natural lightening and pharmacological therapy for treating hyperpigmentation issues because of its effects on melanogenesis ( Bourhim et al, 2018 ; Bourhim et al, 2021 ; ZeinEldin et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Almuhayawi,

Alruhaili,

Gattan

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Active components in medicinal plants provide unlimited useful and traditional medicines. Antimicrobial activities are found in secondary metabolites in plant extracts such as argan oil. This experimental investigation aims to determine argan oil’s volatile compounds and examine their in vitro antimicrobial properties. In silico simulations, molecular docking, pharmacokinetics, and drug-likeness prediction revealed the processes underlying the in vitro biological possessions. Gas chromatography–mass spectrometry (GC/MS) was used to screen argan oil’s primary components. In silico molecular docking studies were used to investigate the ability of the selected bioactive constituents of argan oil to act effectively against Pseudomonas aeruginosa and Staphylococcus aureus (S. aureus) isolated from infections. The goal was to study their ability to interact with both bacteria’s essential therapeutic target protein. The 21 chemicals in argan oil were identified by GC/MS. Docking results for all compounds with S. aureus and P. aeruginosa protease proteins ranged from −5 to −9.4 kcal/mol and −5.7 to −9.7 kcal/mol, respectively, compared to reference ligands. Our docking result indicates that the 10-octadecenoic acid, methyl ester was the most significant compound with affinity scores of −9.4 and −9.7 kcal/mol for S. aureus and P. aeruginosa proteins, respectively. The minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) of argan oil were 0.7 ± 0.03 and 0.5 ± 0.01 for S. aureus and 0.4 ± 0.01 and 0.3 ± 0.02 for P. aeruginosa, respectively. We confirmed the antimicrobial properties of argan oil that showed significant growth inhibition for S. aureus and P. aeruginosa.

show abstract

Elucidation of Melanogenesis-Associated Signaling Pathways Regulated by Argan Press Cake in B16 Melanoma Cells

Cited by 12 publications

References 50 publications

Epidermis-on-a-chip system to develop skin barrier and melanin mimicking model

Epidermis-on-a-chip system to develop skin barrier and melanin mimicking model

Argan (Argania spinosa L.) Seed Oil Cake as a Potential Source of Protein-Based Film Matrix for Pharmaco-Cosmetic Applications

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Contact Info

Product

Resources

About