Elucidation of penetration enhancement mechanism of Emu oil using FTIR microspectroscopy at EMIRA laboratory of SESAME synchrotron

Mansour, Randa S.H.; Sallam, Al-Sayed; Hamdan, Imad I.; Khalil, Enam A.; Yousef, Ibraheem

doi:10.1016/j.saa.2017.05.026

Cited by 24 publications

(14 citation statements)

References 56 publications

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“…Finally, AVO2 displayed significantly ( p < 0.018) lower permeation of the epidermis-dermis layer, probably due to the increased palmitic acid levels present within this oil [ 91 , 100 ]. Avocado oil contains higher concentrations saturated fatty acids compared to the other oils, particularly saturated fatty acids (e.g., palmitic and stearic acids) with longer carbon chain lengths (>C-12) that are known to be less effective penetration enhancers.…”

Section: Resultsmentioning

confidence: 99%

Development of a Self-Emulsifying Drug Delivery System for Optimized Topical Delivery of Clofazimine

2020

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A quality-by-design and characterization approach was followed to ensure development of self-emulsifying drug delivery systems (SEDDSs) destined for topical delivery of the highly lipophilic clofazimine. Solubility and water-titration experiments identified spontaneous emulsification capacity of different excipient combinations and clofazimine. After identifying self-emulsification regions, check-point formulations were selected within the self-emulsification region by considering characteristics required to achieve optimized topical drug delivery. Check-point formulations, able to withstand phase separation after 24 h at an ambient temperature, were subjected to characterization studies. Experiments involved droplet size evaluation; size distribution; zeta-potential; self-emulsification time and efficacy; viscosity and pH measurement; cloud point assessment; and thermodynamic stability studies. SEDDSs with favorable properties, i.e., topical drug delivery, were subjected to dermal diffusion studies. Successful in vitro topical clofazimine delivery was observed. Olive oil facilitated the highest topical delivery of clofazimine probably due to increased oleic acid levels that enhanced stratum corneum lipid disruption, followed by improved dermal clofazimine delivery. Finally, isothermal microcalometric experiments studied the compatibility of excipients. Potential interactions were depicted between argan oil and clofazimine as well as between Span®60 and argan-, macadamia- and olive oil, respectively. However, despite some mundane incompatibilities, successful development of topical SEDDSs achieved enhanced topical clofazimine delivery.

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Section: Resultsmentioning

confidence: 99%

Development of a Self-Emulsifying Drug Delivery System for Optimized Topical Delivery of Clofazimine

2020

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“…To this end, the properties of the lipids in both types of fibres were evaluated using synchrotron‐based Fourier transform infrared microspectroscopy (μ‐FTIR). The combination of a conventional infrared spectrometer with the synchrotron infrared source and a microscope allows the spatial determination of the chemical composition of the samples, and several studies have been performed using μ‐FTIR . Differences in the lipid order or quantity in both types of hair were examined.…”

Section: Introductionmentioning

confidence: 99%

“…The combination of a conventional infrared spectrometer with the synchrotron infrared source and a microscope allows the spatial determination of the chemical composition of the samples, and several studies have been performed using μ-FTIR. 3,15,16 Differences in the lipid order or quantity in both types of hair were examined. In previous studies, attenuated total reflection Fourier transform infrared (ATR-FTIR) was used to study the differences in the amounts of lipids and the lipid order in hair fibres.…”

mentioning

confidence: 99%

Lipid loses and barrier function modifications of the brown‐to‐white hair transition

Oliver

Martı́

Coderch

et al. 2019

Skin Research and Technology

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Background The main objective of this study was to determine the lipid profile of brown and white Caucasian hair fibres and the effects of lipids on the properties of fibres. Materials and Methods To determine the structures of white and brown hair lipid bilayers, cross sections of fibres of both hair types were examined using synchrotron‐based μ‐FTIR mapping. Dynamic vapour sorption (DVS) analyses were also performed to determine the differences in the barrier function of both fibres. Results Spatial identification of lipids showed that a great amount of lipids was present in the medulla of fibres of both hair types, but important differences were also observed between cuticles of the different fibres. The cuticle of a white hair fibre showed a significant decrease in its lipid content, but did not show differences in the lateral packing order with respect to the cuticle of a brown hair fibre. The cortex and medulla of the white hair fibre also exhibited a significant decrease in its lipid content but with a higher lateral packing order than brown hair. Using DVS analysis, it was found that the water dynamics of white hair fibres differed from those of brown hair fibres, showing a decrease in their total capacity to absorb water and an increase in the velocity of the exchange of water with the environment. Conclusion The results of both techniques demonstrated a high correlation between the characteristics of the lipids located in the cuticle and the water dynamics of the fibres.

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“…To this end, the cuticle lipid properties will be evaluated by synchrotron‐based Fourier transform infrared microspectroscopy (µ‐FTIR). The combination of a conventional infrared spectrometer with a synchrotron infrared source and microscope allows for a spatial determination of the chemical composition of the samples, and several studies have been performed using μ‐FTIR 19,20 . Differences in the lipid order or amount will be studied by a combination of the microscopy transmittance FTIR with an ALBA synchrotron infrared light source.…”

Section: Introductionmentioning

confidence: 99%

Ethnic hair: Thermoanalytical and spectroscopic differences

Oliver

Coderch

Carrer

et al. 2020

Skin Research and Technology

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Hair is a protein fibre (85%) and contains low levels of lipids (1%-5%) and water (10%). Hair consists of a follicle and a shaft. 1 The shaft, the part of the hair that is visible above the skin, has a width of 50-100 μm and is composed of three main parts: the medulla, the cortex and the cuticle. The medulla, which possesses a diameter of 5-10 μm, is composed of loosely packed cells and is not always presented in the hair. The cortex, which is 45-90 μm thick, accounts for 90% of the total hair mass. Seven to ten tetramer spiral units, known as intermediate filaments, compose the microfibrils of the cortex. The intermediate filaments are surrounded by a matrix of high-glycine/tyrosine proteins. The proteins interact with the intermediate filaments through intermolecular disulphide bonds that provide inertness, rigidity and high mechanical strength to the hair fibres. 1,2 The cuticle, at the surface of the hair, has a 5-µm-thick protective covering consisting of superimposed layers of flat overlapping structures. 3,4 Low levels of lipids form part of the intercellular membrane complex, and these lipids are presented in the cortex and in the cuticle. In addition, the hair medulla also contains a fraction of rich lipids. 5,6 The presence of cuticles is fundamental to maintain the internal water content of the

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Elucidation of penetration enhancement mechanism of Emu oil using FTIR microspectroscopy at EMIRA laboratory of SESAME synchrotron

Cited by 24 publications

References 56 publications

Development of a Self-Emulsifying Drug Delivery System for Optimized Topical Delivery of Clofazimine

Development of a Self-Emulsifying Drug Delivery System for Optimized Topical Delivery of Clofazimine

Lipid loses and barrier function modifications of the brown‐to‐white hair transition

Ethnic hair: Thermoanalytical and spectroscopic differences

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