2019
DOI: 10.1016/j.jpba.2018.09.031
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Elucidation of pharmacokinetics of novel DNA ligase I inhibitor, S012-1332 in rats: Integration of in vitro and in vivo findings

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Cited by 11 publications
(8 citation statements)
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“…14 Plasma stability was performed in fresh plasma collected from non-treated rats. Microsomal stability was performed in RLM prepared in house and the conditions are similar to as reported by Riyazuddin et al 15 Briey, the contents of reaction mixture are RLM (0.5 mg mL À1 ), 40 mM MgCl 2 , 1 mM NADPH in 0.8 mL of 50 mM Tris-HCl buffer (pH 7.4). All the stability studies were performed at 37 C in a shaking water bath incubator and the reactions were initiated by spiking 100 mg mL À1 of COEE into respective reaction media.…”
Section: Methods Validationmentioning
confidence: 99%
“…14 Plasma stability was performed in fresh plasma collected from non-treated rats. Microsomal stability was performed in RLM prepared in house and the conditions are similar to as reported by Riyazuddin et al 15 Briey, the contents of reaction mixture are RLM (0.5 mg mL À1 ), 40 mM MgCl 2 , 1 mM NADPH in 0.8 mL of 50 mM Tris-HCl buffer (pH 7.4). All the stability studies were performed at 37 C in a shaking water bath incubator and the reactions were initiated by spiking 100 mg mL À1 of COEE into respective reaction media.…”
Section: Methods Validationmentioning
confidence: 99%
“…Gastrointestinal stability was performed in different pH conditions of gastric intestinal simulated media fluids such as FaSSGF (pH‐1.6) and FaSSIF (pH‐6.8). [ 22 ] In which media was prepared according to previously reported protocols containing pepsin (0.1 mg/mL) for FaSSGF and pancreatin (10 mg/mL) for FaSSIF, respectively. This stability study is essential for oral pharmacokinetic study, the drug is administered via an oral route and distributed to the GIT site.…”
Section: Methodsmentioning
confidence: 99%
“…P eff,rat = Q in * In( C in / C out )/2πrL equation for calculating the effective permeability in the small intestine of the rat (Riyazuddin, Husain, et al, 2019). Where Q in is the flow rate (0.2 ml/min), C out is the outlet concentration of perfusate after correction, C in is the concentration of entering of perfusate, r is the inner radius of the intestine (0.18 cm) and L is the length of the intestine (Riyazuddin, Valicherla, et al, 2019).…”
Section: Evaluation Of the Effect Of P‐gp On Drug Permeabilitymentioning
confidence: 99%