“…By using RSI, selenomethionine [120], difluoromethionine [125], trifluoromethionine [126], norleucine [123,127], homopropargylglycine (Hpg) [128], azidohomoalanine (Aha) [129], homoallylglycine (Hag) [130], and methoxinine [127] have been incorporated using methionyl-tRNA synthetase (MetRS); per-thiaproline, 3 [131,132]; trifluorovaline (TfV), 2-amino-3-methyl-4-pentenoic acid have been incorporated using isoleucyl-tRNA synthetase (IleRS) [133][134][135]; o-fluorophenylalanine, m-fluorophenylalanine, pFF [136], 2-pyridylalanine, 3-pyridylalanine, and 4-pyridylalanine [137] have been incorporated using PheRS; 4-aminotryptophan (4AW), 5-aminotryptophan (5AW), 4-fluorotryptophan (4FW), 6-fluorotryptophan (6FW), 5-hydroxytryptophan (5HW), 7AzW have been incorporated using TyrRS [138][139][140][141]; and trifluoroleucine has been incorporated using leucyl-tRNA synthetase (LeuRS) [142,143] in E. coli (Table 12.3). Budisa and coworkers have explored RSI for the integration of Hpg and norleucine into the superoxide dismutase (SOD) protein in yeast [123].…”