Elucidation of the Antigenic Architecture of the Acholeplasma laidlawii Cell Membrane by Immunoabsorption in Combination with Crossed Immunoelectrophoresis

Comparison between Mycoplasma bovoculi strains by electrophoretic and immunoblotting techniques: identification of membrane proteins involved in adherence

“…Growth of the organism in medium containing unsaturated fatty acids resulted in release of membrane proteins into the medium. In these experiments, cells harvested at early growth phase exhibited greater expression of antigenic determinants on the surface membrane than at later stages as detected by immunoabsorption and surface iodination (36). The use of a dialysate medium prepared from soy peptone and fresh yeast alle viates the problem of medium contamination for many mycoplasmas (42).…”

“…Antigenic determinants of protein were expressed on the external surface while those of and proteins were on the internal surface. protein expressed no antigenic determinants on either sur face (36). An actin-like protein has been extracted from M. pneumoniae by alkaline treatment (60) in analogy to rabbit muscle F-actin (54).…”