Elucidation of the enzyme involved in 2,3,5-triphenyl tetrazolium chloride (TTC) staining activity and the relationship between TTC staining activity and fermentation profiles in Saccharomyces cerevisiae

Tanaka, Jumpei; Kiyoshi, Keiji; Kadokura, Toshimori; Suzuki, Ken‐ichiro; Nakayama, Shunichi

doi:10.1016/j.jbiosc.2020.12.001

Cited by 29 publications

(13 citation statements)

References 24 publications

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“…These observations raised the question of whether light exposure affects redox metabolism in P. aeruginosa . To test this, we adapted an assay based on reduction of the redox indicator triphenyl tetrazolium chloride (TTC) ( 33 – 36 ). TTC is colorless in its oxidized form; it is reduced by components of the electron transport chain and subsequently forms a red precipitate ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms

Kahl

Eckartt

Morales

et al. 2022

mBio

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Section: Resultsmentioning

confidence: 99%

Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms

Kahl

Eckartt

Morales

et al. 2022

mBio

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“…The cerebral infarction volume in rats was measured using TTC staining [ 18 ]. First, the brain tissue was obtained and cut into slices with 1.5 mm in thickness.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of phillyrin against cerebral ischemia/reperfusion injury in rats and oxidative stress-induced cell apoptosis and autophagy in neurons

Chen

Zhang

et al. 2022

Bioengineered

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This study explored the role and potential molecular mechanism of phillyrin in cerebral ischemia/reperfusion (I/R) injury. The rat middle cerebral artery occlusion (MCAO)/R model was constructed, and cerebral infarction volume, brain water content, and neurological score were measured. Neuron morphological structures in brain tissues and primary neuron apoptosis were detected using hematoxylin and eosin (H&E) staining and Hoechst 33258 staining, respectively. In MCAO/R rats, phillyrin markedly reduced cerebral infarction volume, neurological score, and brain water content and inhibited neuron apoptosis. In vitro experiments showed that phillyrin remarkably increased viability and decreased lactate dehydrogenase (LDH) release of H 2 O 2 -injured neurons. Moreover, phillyrin remarkably downregulated the proportion of apoptosis-related protein B-associated X (Bax)/B-cell lymphoma protein 2 (Bcl-2) and reduced procaspase-3, phospho-Akt (p-Akt-1), and phosphorylation-mammalian target of rapamycin (p-mTOR) levels in H 2 O 2 -injured neurons. Furthermore, phosphatidylinositol-3 kinase (PI3K) inhibitor ZSTK474 weakened the effects of phillyrin on p-mTOR, p-Akt-1, characteristic proteins of autophagy 3-II (LC3-II) and beclin-1 levels, and H 2 O 2 -induced neuronal apoptosis and autophagy. Taken together, phillyrin alleviates I/R injury by inhibiting neuronal cell apoptosis and autophagy pathway, which may provide a new treatment strategy for cerebral I/R injury.

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“…To visualize O 2 .- and root activity in plants in situ , nitroblue tetrazolium (NBT) and 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining was performed, respectively, as described previously ( Kong et al, 2018 ; Tanaka et al, 2020 ) and modified slightly. O 2 .- generated in seedlings was measured by incubating the plants in 1% NBT within 20 mmol L −1 potassium phosphate, washing with distilled water, and decolorizing with 70% ethanol solution in water at 90°C for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Bismuth Vanadium Oxide Can Promote Growth and Activity in Arabidopsis thaliana

et al. 2021

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The excellent properties of nanomaterials have been confirmed in many fields, but their effects on plants are still unclear. In this study, different concentrations of bismuth vanadate (BV) were added to the growth medium to analyze the growth of seedlings, including taproots, lateral roots, leaf stomata, root activity, and superoxide anion O2.- generation. Gene expression levels related to root growth were determined by quantitative PCR in Arabidopsis thaliana. The results showed that BV promoted the growth of taproots and the development of lateral roots, enhanced the length of the extension zone in roots, increased the number and size of leaf stomata and root activity, reduced the accumulation of ROS in seedlings, and changed the expression levels of genes related to polyamines or hormones. At the same time, we investigated the antibacterial activity of BV against a variety of common pathogens causing crop diseases. The results showed that BV could effectively inhibit the growth of Fusarium wilt of cotton and rice sheath blight. These results provide a new prospect for the development of nanomaterial-assisted plants, which is expected to become one of the ways to solve the problem of controlling and promoting the development of plants. At the same time, it also provides a reference for the study of the effect of BV on plants.

show abstract

Elucidation of the enzyme involved in 2,3,5-triphenyl tetrazolium chloride (TTC) staining activity and the relationship between TTC staining activity and fermentation profiles in Saccharomyces cerevisiae

Cited by 29 publications

References 24 publications

Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms

Light/Dark and Temperature Cycling Modulate Metabolic Electron Flow in Pseudomonas aeruginosa Biofilms

Protective effect of phillyrin against cerebral ischemia/reperfusion injury in rats and oxidative stress-induced cell apoptosis and autophagy in neurons

Bismuth Vanadium Oxide Can Promote Growth and Activity in Arabidopsis thaliana

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