Elucidation of the growth delimitation of Dunaliella tertiolecta under nitrogen stress by integrating transcriptome and peptidome analysis

Shin, HyeonSeok; Hong, Seong-Joo; Kim, Hyojin; Yoo, Chan Yul; Lee, Hookeun; Choi, Hyung‐Kyoon; Lee, Choul-Gyun; Cho, Byung-Kwan

doi:10.1016/j.biortech.2015.07.002

Cited by 43 publications

(35 citation statements)

References 35 publications

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“…2c). This is similar to other researchers who observe similar declines in total fatty acid content in D. tertiolecta under N-depletion (7.5% of DCW in N-sufficient vs 5.9% of DCW in N-deficient)29. The parallel increase in neutral lipids could be explained by a dramatic shift in intracellular processes, from the catabolic degradation of thylakoid membranes (which contribute to total lipids) to the anabolic reactions of storage compound accumulation (neutral lipids and starch content).…”

Section: Discussionsupporting

confidence: 92%

“…In 2011, Rismani-Yazdi et al provided a preliminary annotation of available genes and putative pathways in D. tertiolecta based on sequence similarity between Chlamydomonas reinhardtii and Volvox carteri 27, but did not compare the transcriptomic response between physiological states. Recently, several new transcriptomic studies of Dunaliella species have been conducted282930. Yao et al created a draft database for annotation of RNA-seq data from D. tertiolecta and described pathway analyses of a random mutant with enhanced lipid production28.…”

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confidence: 99%

“…Yao et al created a draft database for annotation of RNA-seq data from D. tertiolecta and described pathway analyses of a random mutant with enhanced lipid production28. Similarly, Shin et al independently annotated the transcriptome of D. tertiolecta and studied possible genes related to growth limitation during N-depletion29.…”

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confidence: 99%

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Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta

Tan

Lin

Shen

et al. 2016

Sci Rep

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Certain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this study, the microalga Dunaliella tertiolecta was used as a model to study the global gene expression profile regulating starch accumulation in microalgae. D. tertiolecta, when depleted of nitrogen, produced only 1% of dry cell weight (DCW) in neutral lipids, while starch was rapidly accumulated up to 46% DCW. The increased in starch content was accompanied by a coordinated overexpression of genes shunting carbon towards starch synthesis, a response not seen in the oleaginous microalgae Nannochloropsis oceanica, Chlamydomonas reinhardtii or Chlorella vulgaris. Genes in the central carbon metabolism pathways, particularly those of the tricarboxylic acid cycle, were also simultaneously upregulated, indicating a robust interchange of carbon skeletons for anabolic and catabolic processes. In contrast, fatty acid and triacylglycerol synthesis genes were downregulated or unchanged, suggesting that lipids are not a preferred form of storage in these cells. This study reveals the transcriptomic influence behind storage reserve allocation in D. tertiolecta and provides valuable insights into the possible manipulation of genes for engineering microorganisms to synthesize products of interest.

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Section: Discussionsupporting

confidence: 92%

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confidence: 99%

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Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta

Tan

Lin

Shen

et al. 2016

Sci Rep

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“…1D). These results are similar to the stress response of other microalgae such as Chlamydomonas reinhardtii and Dunaliella tertiolecta , where nutrition deficient stress led to carbohydrate accumulation1617. On the other hand, the FAME content of the cells cultured in low and high temperature decreased by 2.9% and 4.1% for cells cultured in low and high temperature respectively, compared to the cells cultured in mid- temperature condition (Fig.…”

Section: Resultssupporting

confidence: 84%

“…To remove any false positives that may have occurred from the large input data of 16.0 Gb, the assembled transcripts were subjected to BLASTX analysis against the non-redundant (Nr) database2425. After a stringent E -value cutoff of less than 10 −10 , the length coverage of 80% was used to obtain the full-length transcripts17. These stringent cutoff values resulted in a reduction of the total assembled bases to 31.5 Mb with 26,245 transcripts remaining (Supplemental Table S2).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae

Shin

Hong

Yoo

et al. 2016

Sci Rep

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Temperature is a critical environmental factor that affects microalgal growth. However, microalgal coping mechanisms for temperature variations are unclear. Here, we determined changes in transcriptome, total carbohydrate, total fatty acid methyl ester, and fatty acid composition of Tetraselmis sp. KCTC12432BP, a strain with a broad temperature tolerance range, to elucidate the tolerance mechanisms in response to large temperature variations. Owing to unavailability of genome sequence information, de novo transcriptome assembly coupled with BLAST analysis was performed using strand specific RNA-seq data. This resulted in 26,245 protein-coding transcripts, of which 83.7% could be annotated to putative functions. We identified more than 681 genes differentially expressed, suggesting an organelle-specific response to temperature variation. Among these, the genes related to the photosynthetic electron transfer chain, which are localized in the plastid thylakoid membrane, were upregulated at low temperature. However, the transcripts related to the electron transport chain and biosynthesis of phosphatidylethanolamine localized in mitochondria were upregulated at high temperature. These results show that the low energy uptake by repressed photosynthesis under low and high temperature conditions is compensated by different mechanisms, including photosystem I and mitochondrial oxidative phosphorylation, respectively. This study illustrates that microalgae tolerate different temperature conditions through organelle specific mechanisms.

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Köse²

2017

Encyclopedia of Sustainability Science and Technology

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Elucidation of the growth delimitation of Dunaliella tertiolecta under nitrogen stress by integrating transcriptome and peptidome analysis

Cited by 43 publications

References 35 publications

Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta

Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta

Genome-wide transcriptome analysis revealed organelle specific responses to temperature variations in algae

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