Elucidation of the Regular Emission Mechanism of Volatile β-Ocimene with Anti-insect Function from Tea Plants (<i>Camellia sinensis</i>) Exposed to Herbivore Attack

Jian, Guotai; Jia, Yongxia; Li, Jianlong; Zhou, Xiaochen; Liao, Yinyin; Dai, Guangyi; Zhou, Ying; Tang, Jinchi; Zeng, Lanting

doi:10.1021/acs.jafc.1c03534

Cited by 24 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mated wasps need to locate hosts and lay eggs, so the odour cues are extremely important. It was demonstrated that tea plants emitted β-ocimene upon mechanical damage or herbivore feeding (Chen et al, 2021;Jian et al,…”

Section: Discussionmentioning

confidence: 99%

Plant‐derived monoterpene S‐linalool and β‐ocimene generated by CsLIS and CsOCS‐SCZ are key chemical cues for attracting parasitoid wasps for suppressing Ectropis obliqua infestation in Camellia sinensis L

Liu,

Wang,

Chen

et al. 2024

Plant Cell & Environment

View full text Add to dashboard Cite

Insect‐induced plant volatile organic compounds (VOCs) may function as either direct defence molecules to deter insects or indirect defence signals to attract the natural enemies of the invading insects. Tea (Camellia sinensis L.), an important leaf‐based beverage crop, is mainly infested by Ectropis obliqua which causes the most serious damage. Here, we report a mechanistic investigation of tea plant‐derived VOCs in an indirect defence mechanism against E. obliqua. Parasitoid wasp Parapanteles hyposidrae, a natural enemy of E. obliqua, showed strong electrophysiological response and selection behaviour towards S‐linalool and β‐ocimene, two monoterpenes with elevated emission from E. obliqua‐damaged tea plants. Larvae frass of E. obliqua, which also released S‐linalool and β‐ocimene, was found to attract both mated female or male Pa. hyposidrae according to gas chromatography‐electroantennogram detection and Y‐tube olfactometer assays. In a field setting, both S‐linalool and β‐ocimene were effective in recruiting both female and male Pa. hyposidrae wasps. To understand the molecular mechanism of monoterpenes‐mediated indirect defence in tea plants, two novel monoterpene synthase genes, CsLIS and CsOCS‐SCZ, involved in the biosynthesis of S‐linalool or β‐ocimene, respectively, were identified and biochemically characterised. When the expression of these two genes in tea plants was inhibited by antisense oligodeoxynucleotide, both volatile emission and attraction of wasps were reduced. Furthermore, gene expression analysis suggested that the expression of CsLIS and CsOCS‐SCZ is regulated by the jasmonic acid signalling pathway in the tea plant.

show abstract

Section: Discussionmentioning

confidence: 99%

Plant‐derived monoterpene S‐linalool and β‐ocimene generated by CsLIS and CsOCS‐SCZ are key chemical cues for attracting parasitoid wasps for suppressing Ectropis obliqua infestation in Camellia sinensis L

Liu,

Wang,

Chen

et al. 2024

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…In comparison, the anti-insect function of some volatiles might be achieved through the upstream signal molecule JA [ 66 ]. It is also proven that β-ocimene released from tea ( Camellia sinensis ) leaves does not affect tea geometrid ( Ectropis grisescens Warren) directly but may change the phytohormone synthesis by fumigating with tea leaves [ 67 ]. Although we have demonstrated that β-ocimene synthesized by GmOCS has an anti-insect function in both soybean and tobacco ( Figure 5 and Figure 6 ), its accurate anti-insect mechanism remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of a (E)-β-Ocimene Synthase Gene Contributing to the Defense against Spodoptera litura

Han

Shao

Gao

et al. 2023

IJMS

View full text Add to dashboard Cite

Soybean is a worldwide crop that offers valuable proteins, fatty acids, and phytonutrients to humans but is always damaged by insect pests or pathogens. Plants have captured sophisticated defense mechanisms in resisting the attack of insects and pathogens. How to protect soybean in an environment- or human-friendly way or how to develop plant-based pest control is a hotpot. Herbivore-induced plant volatiles that are released by multiple plant species have been assessed in multi-systems against various insects, of which (E)-β-ocimene has been reported to show anti-insect function in a variety of plants, including soybean. However, the responsible gene in soybean is unknown, and its mechanism of synthesis and anti-insect properties lacks comprehensive assessment. In this study, (E)-β-ocimene was confirmed to be induced by Spodoptera litura treatment. A plastidic localized monoterpene synthase gene, designated as GmOCS, was identified to be responsible for the biosynthesis of (E)-β-ocimene through genome-wide gene family screening and in vitro and in vivo assays. Results from transgenic soybean and tobacco confirmed that (E)-β-ocimene catalyzed by GmOCS had pivotal roles in repelling a S. litura attack. This study advances the understanding of (E)-β-ocimene synthesis and its function in crops, as well as provides a good candidate for further anti-insect soybean improvement.

show abstract

“…( E )-β-ocimene in flower and leaves of pear has multiple ecological roles in attracting pollinators and plant-pest interactions ( Scutareanu et al., 2003 ; Su et al., 2022 ). The TPS in catalyzing ( E )-β-ocimene biosynthesis, namely ( E )-β-ocimene synthase, has been functionally characterized in several plant species such as Antirrhinum majus ( Dudareva et al., 2003 ), Arabidopsis thaliana ( Fäldt et al., 2003 ), Camellia sinensis ( Chen et al., 2021 ; Jian et al, 2021 ), Citrus unshiu ( Shimada et al., 2005 ), Lotus japonicus ( Arimura et al., 2004 ), Malus domestica ( Nieuwenhuizen et al., 2013 ), Matricaria recutita ( Irmisch et al., 2012 ), Medicago truncatula ( Navia-Giné et al., 2009 ), Morus notabilis ( Ding et al., 2020 ), Nicotiana attenuata ( Xu et al., 2020 ), Osmanthus fragrans ( Zeng et al., 2015 ), Phaseolus lunatus ( Arimura et al., 2008 ), Populus trichocarpa ( Irmisch et al., 2014 ) and Vitis vinifera ( Martin et al., 2010 ). In the current study, the ( E )-β-ocimene synthase gene PbeOCS was present in the RNA-seq data and functionally characterized in vitro ( Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Functional characterization of a terpene synthase responsible for (E)-β-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory

Huang

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

(E)-β-ocimene, a ubiquitous monoterpene volatile in plants, is emitted from flowers to attract pollinators and/or from vegetative tissues as part of inducible defenses mediated by complex signaling networks when plants are attacked by insect herbivores. Wild pear species Pyrus betuleafolia used worldwide as rootstock generally displays valuable pest-resistant traits and is a promising genetic resource for pear breeding. In the current study, transcriptional changes in this wild pear species infested with a polyphagous herbivore Spodoptera litura and the underlying molecular mechanisms were fully investigated. A total of 3,118 differentially expressed genes (DEGs) were identified in damaged pear leaf samples. Spodoptera litura larvae infestation activated complex phytohormonal signaling networks in which jasmonic acid, ethylene, brassinosteroids, cytokinin, gibberellic acid and auxin pathways were induced, whereas salicylic acid and abscisic acid pathways were suppressed. All DEGs associated with growth-related photosynthesis were significantly downregulated, whereas most DEGs involved in defense-related early signaling events, transcription factors, green leaf volatiles and volatile terpenes were significantly upregulated. The PbeOCS (GWHGAAYT028729), a putative (E)-β-ocimene synthase gene, was newly identified in P. betuleafolia transcriptome. The upregulation of PbeOCS in S. litura-infested pear leaves supports a potential role for PbeOCS in herbivore-induced plant defenses. In enzyme-catalyzed reaction, recombinant PbeOCS utilized only geranyl pyrophosphate but not neryl diphosphate, farnesyl pyrophosphate or geranylgeranyl diphosphate as a substrate, producing (E)-β-ocimene as the major product and a trace amount of (Z)-β-ocimene. Moreover, as a catalytic product of PbeOCS, (E)-β-ocimene showed repellent effects on larvae of S. litura in dual-choice bioassays. What is more, (E)-β-ocimene increased mortalities of larvae in no-choice bioassays. These findings provide an overview of transcriptomic changes in wild pears in response to chewing herbivores and insights into (E)-β-ocimene biosynthesis in pear plants, which will help elucidate the molecular mechanisms underlying pear-insect interactions.

show abstract

Elucidation of the Regular Emission Mechanism of Volatile β-Ocimene with Anti-insect Function from Tea Plants (Camellia sinensis) Exposed to Herbivore Attack

Cited by 24 publications

References 45 publications

Plant‐derived monoterpene S‐linalool and β‐ocimene generated by CsLIS and CsOCS‐SCZ are key chemical cues for attracting parasitoid wasps for suppressing Ectropis obliqua infestation in Camellia sinensis L

Plant‐derived monoterpene S‐linalool and β‐ocimene generated by CsLIS and CsOCS‐SCZ are key chemical cues for attracting parasitoid wasps for suppressing Ectropis obliqua infestation in Camellia sinensis L

Functional Characterization of a (E)-β-Ocimene Synthase Gene Contributing to the Defense against Spodoptera litura

Functional characterization of a terpene synthase responsible for (E)-β-ocimene biosynthesis identified in Pyrus betuleafolia transcriptome after herbivory

Contact Info

Product

Resources

About