Elutability of Proteins from Aluminum-Containing Vaccine Adjuvants by Treatment with Surfactants

Effects of solution conditions and surface chemistry on the adsorption of three recombinant botulinum neurotoxin antigens to aluminum salt adjuvants

“…However, previous studies have suggested that the presence of phosphate in solution may alter adsorption of proteins to aluminum hydroxide due to exchange of phosphate for hydroxyl groups on the adjuvant surface. 30,[41][42][43][44][45][46] In an effort to understand the influence of solution phosphate on adsorption in our system, experiments were performed in which phosphate concentrations were increased while pH was held constant. The results (Fig.…”

“…10,11 Vaccines based on recombinant proteins often require adjuvants in order to obtain an adequate immune response. [12][13][14] Aluminum salts are the most commonly utilized adjuvants in the United States for use in human vaccine products, [15][16][17] although there are other adjuvants under clinical investigation. 18 Several mechanisms have been proposed to explain the increase in potency of vaccines delivered with aluminum salt adjuvants.…”

Effect of pH on the Elution of Model Antigens from Aluminum-Containing Adjuvants

“…However, during the development of vaccines or during the quality control of vaccines it is frequently desirable to elute the antigen from the aluminum-containing adjuvant in order to directly determine the amount of adsorbed antigen or to examine its conformation. Although the elution of antigens from aluminum-containing adjuvants is an important issue, only two studies have been reported which address this subject (2,3).…”

Comparable quality attributes of hepatitis E vaccine antigen with and without adjuvant adsorption-dissolution treatment

“…21 No significant changes were observed in protein antigens eluted from the aluminum-containing adjuvants using optimized antigen recovery methods including the use of chelating agents and surfactants. 18,[22][23][24] Cryo-transmission electron microscopy (Cryo-EM) and binding analyses of VLP antigens in GARDASIL Ò , the quadrivalent human papillomavirus (HPV) VLP vaccine, showed no apparent changes in their spherical shape and key epitopes of VLP antigens when adsorbed onto adjuvants and after dissolution of amorphous adjuvants. 25,39 Comparable physiochemical properties of the recovered HBsAg antigen of Hepatitis B virus versus the antigen in aqueous solution were shown using multiple biophysical methods.…”