Embedding in Epoxy Resins for Ultrathin Sectioning in Electron Microscopy

Richardson, K. C.; Jarett, Leonard; Finke, Edward H.

doi:10.3109/10520296009114754

Cited by 3,087 publications

(950 citation statements)

References 6 publications

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“…After rinsing in 0.1 M cacodylate buffer, tissue samples were postfixed in unbuffered 1% osmium tetroxide for 2 hr and additionally postfixed in 1% uranyl acetate, dehydrated in a graded series of ethanol, embedded in epoxy resin, and cut with Diatome diamond knives. Single and serial semithin sections (thickness 1.0 m) for light microscopy (LM) were stained with methylene blue-azur II (Richardson et al, 1960). Ultrathin sections (thickness 70 nm) for transmission electron microscopy (TEM) were collected on Formvar-coated slot grids, uranyl acetate, lead citrate double stained using the LKB 2168 Ultrastainer, and observed under a Zeiss EM 902 transmission electron microscope.…”

Section: Methodsmentioning

confidence: 99%

The dorsal lingual epithelium of Rhinoclemmys pulcherrima incisa (Chelonia, Cryptodira)

2004

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This study employed light microscopic (LM), scanning electron microscopic (SEM), and transmission electron microscopic (TEM) methods to provide detailed morphological information on the histological and ultrastructural features of the dorsal tongue epithelium of Rhinoclemmys pulcherrima incisa. SEM revealed columnar papillae laterally, as well as papillae, which tend to have a ridge-like appearance in the center of the tongue. LM and TEM showed three different zones of lingual epithelium: a stratified apical area with serous cells at the top of the papillae, a stratified lateral area with both serous and mucus cells, and an unstratified glandular area consisting of distinct glandular ducts with mucus cells. Comparison with morphological data from other turtles shows that the lingual epithelial structure in R. p. incisa is in accordance with that observed for other generalized omnivores which prefer a terrestrial lifestyle, thus matching the ecological information about this species. Anat Rec Part A 277A: 227-235, 2004. © 2004 Key words: turtle; tongue; lingual glands; histology; electron microscopy Within chelonians, the outline of the tongue, the dorsal tongue topography, and the presence, form, and distribution of lingual papillae show significant variability (Winokur, 1988;Weisgram et al

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Section: Methodsmentioning

confidence: 99%

The dorsal lingual epithelium of Rhinoclemmys pulcherrima incisa (Chelonia, Cryptodira)

2004

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“…Cells fixed in Carnoy's solution were stained for DNA and R N A with methyl green-pyronin (28). Papanicolaou staining (29) was performed on cells wet-fixed in 95% ethanol, and methylene blue staining (30) was performed on cells that were processed as for routine electron microscopy (see below). A commercial kit (At386A; Sigma) was used for acid phosphatase staining.…”

Section: Methodsmentioning

confidence: 99%

Histologic and electron microscopic characterization of the antiperinuclear factor antigen

Vivino

Maul

1990

Arthritis & Rheumatism

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The presence of the antiperinuclear factor, an autoantibody that recognizes cytoplasmic antigens, was detected in sera from patients with rheumatoid arthritis (59%), seronegative rheumatoid arthritis (36%), systemic lupus erythematosus (46%), systemic sclerosis (a%), and in nonautoimmune controls (10%). The antigenic perinuclear granules were found in the stratum intermedium layer of the buccal mucosa. Granules exhibited histologic features of nucleoproteins, stained for ribonucleoprotein, and showed the ultrastructural characteristics of aggregated rough endoplasmic reticulum. The antiperinuclear factor may recognize a common autoantigen in connective tissue disease.Rheumatoid arthritis (RA) patients, similar to those with related disorders, can form a variety of autoantibodies against nuclear, cytoplasmic, and tissue-specific antigens ( 1,2). Antiperinuclear factor (APF). first described in the mid-1960s by Nienhuis and Mandema (3). is an autoantibody that recognizes cytoplasmic granules in human buccal mucosal cells. geneous-appearing granules, 0.54.0 pm in diameter, located in the cytoplasm around the nucleus. Investigators have found APF in the sera of 50436% of RA patients (4-15), 31-3% of patients with Sjogren's syndrome (5,9,13), as many as 10% of healthy controls, and rarely in other rheumatic disease patients (3-7). A recent study suggested that APF determination in rheumatic disease patients by indirect immunofluorescence had a sensitivity and specificity for RA of 86% and 96%. respectively (7). The presence of APF correlates most closely with the presence of rheumatoid factor and rheumatoid nodules, but the antibody has also been detected in 37-76% of patients with seronegative RA (5-8).The APF antibody binds to cytoplasmic granules in human buccal mucosal cells, which have presumptively been termed "keratohyaline granules" (4.16) based on their rough histologic resemblance to the keratohyaline bodies in the stratum granulosum of human epidermis. The biologic and biochemical nature of the APF-reactive granules is unknown. Attempts to characterize the antigen have thus far been hampered by a lack of availability of suitable human donors (5-7) or alternate animal tissue substrates for the assay (16). However, independent studies in oral medicine (17-20) have documented the presence of at least 3 types of cytoplasmic granules in human oral keratinocytes, including "membrane-coated" granules, "epidermaltype" keratohyaline granules, and "buccal-type'' keratohyaline granules; these findings have not been related to the APF antigen.In this report, we extend our preliminary observations regarding the prevalence of APF in various rheumatic diseases (21) and the staining characteristics of the perinuclear antigen-containing granules (22). The diagnostic specificity of the APF assay (by indi-

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“…Semithin sections for light microscopy observations were stained with methyleneblue-azur II (Richardson et al, 1960). Ultrathin sections were picked up on 200-square mesh grids or formvar-coated slot grids, were uranyl acetate-lead citrate double-stained, and observed under a Zeiss EM 902 (Oberkochen, Germany) transmission electron microscope.…”

Section: Tissue Preparation and Transmission Electron Microscopymentioning

confidence: 99%

Sound‐generating and ‐detecting motor system in catfish: Design of swimbladder muscles in doradids and pimelodids

Ladich

2001

Anat. Rec.

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Catfishes have evolved a diversity of swimbladder muscles serving in the generation of different sounds and probably other acoustic functions. In order to find out if anatomical and acoustical differences are parallelled by fine structural differences, I examined the sonic muscles of the doradid Platydoras and the pimelodid Pimelodus by gross dissections and ultrastructural methods. In Platydoras, the sound-generating (drumming) muscle (DM) inserts on a dorsal bony plate that vibrates the swimbladder. In pimelodids, the large DM attaches directly on the ventral surface of the swimbladder, whereas the small tensor tripodis muscle (TT) inserts on the rostral surface near the tripus, the most caudal Weberian ossicle. Fibers of all three muscles possess an extensive development of sarcoplasmatic reticulum (SR) in association with very thin myofibrils (MF) but differed widely in their arrangement. In Platydoras, ribbons of MFs are arranged radially around a central core. Mitochondria were found within the core and the peripheral sarcoplasm. Pimelodus does not have a differentiated core and the cross-sectional area of DM-MFs is about 15% larger as determined by stereological measurements. The TT possesses shorter sarcomeres and more mitochondria than DMs, which were primarily found between MFs. This suggests faster contraction properties and greater resistence to fatigue compared with sonic muscles. Data indicate that the higher amount of DM-myofibrils in pimelodids might result in stronger muscle contractions and, presumably, in higher sound intensities. The fine structure of the TT reveals that contractions most likely prevent transmission of swimbladder vibrations to the inner ear via the Weberian ossicles during vocalization.

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Embedding in Epoxy Resins for Ultrathin Sectioning in Electron Microscopy

Cited by 3,087 publications

References 6 publications

The dorsal lingual epithelium of Rhinoclemmys pulcherrima incisa (Chelonia, Cryptodira)

The dorsal lingual epithelium of Rhinoclemmys pulcherrima incisa (Chelonia, Cryptodira)

Histologic and electron microscopic characterization of the antiperinuclear factor antigen

Sound‐generating and ‐detecting motor system in catfish: Design of swimbladder muscles in doradids and pimelodids

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