Embedding Media for 1–2 Micron Sectioning. 2. Hydroxyethyl Methacrylate Combined with 2-Butoxyethanol

Ruddell, Craig L.

doi:10.3109/10520296709115020

Cited by 120 publications

(23 citation statements)

References 2 publications

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“…Using both histochemical esterase staining techniques for mast cells, macrophagcs, and polymorphonuclear leukocytes, and electron microscopic studies, we have attempted to identify more preciscly the cell types at cartilage-pannus junctions. The dual problems of sectioning hard tissue and retaining enzyme activity for histochemical reactions werc overcome by using a methacrylate-based embedding mcdium (JB-4, Polysciences) (23,24).…”

Section: Discussionmentioning

confidence: 99%

Histopathology of the rheumatoid lesion

Bromley¹,

Woolley²

1984

Arthritis & Rheumatism

220

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Histochemical and ultrastructural techniques were used to examine the cartilage-pannus junction of 49 rheumatoid joints. Whereas 24 showed relatively acellular, fibrous pannus tissue, 25 demonstrated "cellular" junctions with different cell types at sites of erosion. Macrophages and fibroblasts were commonly observed as majority cell types in most specimens, but others showed mast cells, polymorphonuclear leukocytes, dendritic, or plasma cells as the predominant cell type. Some showed local accumulations of different cell types at sites along each junction. Our findings show that the cellular composition at sites of cartilage erosion varies greatly and suggest that cells are subject to turnover and interchange.Extensive proteolytic degradation of articular cartilage is a characteristic feature of joint destruction in rheumatoid arthritis. This process is generally irreversible and is commonly associatcd with invasive, hypertrophied synovium. Histologic observations have shown that the early rheumatoid lesion is characterized by a proliferation of synovial lining cells with subscquent neovascularization and infiltration by lym-

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Section: Discussionmentioning

confidence: 99%

Histopathology of the rheumatoid lesion

Bromley¹,

Woolley²

1984

Arthritis & Rheumatism

220

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“…Sections of tissues, 250 8, thick, were placed on grids, stained with uranyl acetate and lead citrate [6], and examined with a Philips EM200 electron microscope. Pieces of canine liver fixed in glutaraldehyde were embedded also in butoxyethanolglycol methacrylate [9], stained with GOMORI'S stain for iron, and examined by light microscopy.…”

Section: Methodsmentioning

confidence: 99%

Fate of Erythrocytes Containing Heinz Bodies in the Spleen and Liver of Dogs and Turkeys

Simpson

1971

Vet Pathol

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Abstract. Similar phagocytic and digestive events involving erythrocytes containing HEINZ bodies occured in KUPFFER cells and splenic macrophages of the dog. The earliest stage was confinement of a nonhemolyzed red blood cell by the membrane of a phagocytic vacuole. Next, there was leaching of hemoglobin into the phagocytic vacuole, followed by fragmentation and loss of the erythrocytic membrane. A round phagocytic vacuole containing hemosiderin was the fate of phagocytized erythrocytes containing HEINZ bodies.There were HEINZ bodies in both the cytoplasm and nucleoplasm of turkey erythrocytes. Such erythrocytes occurred in phagocytic vacuoles of KUPFFER cells and splenic macrophages and evolved through a process similar to that occurring in canine tissue, except that a single vacuole often contained one or more erythrocytes. HEINZ bodies were first described in 1890 [3], but there still is not agreement as to their nature and disposition. The popular opinion is that they be an insoluble denaturative product of hemoglobin [4,7,8], be composed of repeating electron-dense units [7], and be removed by the reticuloendothelial system [&lo]. It is known that erythrophagocytosis is accomplished in KUPFFER cells and that the digestion of erythrocytes occurs within phagocytic vacuoles [2]. Erythrocytes containing HEINZ bodies are phagocytized 'in a like manner [8,10]. However, the entire sequential process of disposal of HEINZ bodies in macrophages of the liver and spleen has not been resolved. This paper reports an ultrastructural study in dogs and turkeys of phagocytosis of erythrocytes containing HEINZ bodies and the reconstruction of the orderly procession of events leading to the ultimate fate of HEINZ bodies in macrophages of the liver and spleen.

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“…SEM examination was performed using two models: a JEOL JSM 25SII SEM and a JFC-1100 E Ion Sputtering Device, at Alexandria University, Alexandria, Egypt, and a digital scanning microscope, DSM 940 Zeiss, version (6.38) 2002, at the University of Hohenheim, Stuttgart, Germany. The embedding of samples (flowers at anthesis) in plastic followed the method of Ruddell (1967aRuddell ( , 1967b, modified by Hermanns & Schulz (1981). The terms used by Faegri & Iversen (1975) and Punt et al (1994) for describing the polyad pattern have been adopted here.…”

Section: Methodsmentioning

confidence: 99%

Pollen dimorphism in Acacia asak (Forssk.) Willd. (Fabaceae: Mimosoideae)

Elazab

2015

Palynology

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The study of pollen grains of Acacia asak showed a marked pollen dimorphism of smooth exine (psilate) polyad type A and rough exine (scabrate) polyad type B, when examined using light microscopy (LM) and scanning electron microscopy (SEM). Typical pollen grains of Acacia asak are 16-monads. Both acetolised and non-acetolised grains of the two types were studied, measured, described and illustrated using LM and SEM. The main differences between these and the functional significance are discussed. Polyad dimorphism can be used as a character to distinguish between A. asak and otherAcacia species. In this study, two polyad types are recorded from the same anther of A. asak for the first time, possibly related to pollination function. However, the function of polyad dimorphism in the same anther is still unclear and requires further study.

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Embedding Media for 1–2 Micron Sectioning. 2. Hydroxyethyl Methacrylate Combined with 2-Butoxyethanol

Cited by 120 publications

References 2 publications

Histopathology of the rheumatoid lesion

Histopathology of the rheumatoid lesion

Fate of Erythrocytes Containing Heinz Bodies in the Spleen and Liver of Dogs and Turkeys

Pollen dimorphism in Acacia asak (Forssk.) Willd. (Fabaceae: Mimosoideae)

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