“…If, however, a large injection of non-radioactive B,, was given shortly after the oral test dose of labelled B,,, radioactive material was excreted in the urine of normal subjects, but little or none was detected in the urine of patients with pernicious anaemia unless a potent source of intrinsic factor was given with the labelled BIZ.More recently, Glass, Boyd and Stephanson (1954b) showed that the hepatic uptake of radioactivity, measured externally by a surface scintillation counter, may be used to demonstrate the absorption of orally administered radioactive B, ,.However, it was not possible, using BOCo-labelled B,,, to measure changes in plasma radioactivity after its oral administration (Schilling, 1953). The amount of B,, that can be absorbed, even by normal subjects, from single oral doses is small, (Glass et al 1954b;Callender and Evans, 1955;Booth and Mollin, 1956) and the specific activity of the radioactive vitamin is relatively low when 60Co is used as the label. The recent introduction of vitamin B, , labelled with the shorter-lived isotopes of cobalt (Bradley, Smith, Baker and Mollin, 1954; Mollin and Smith, 1955) has made possible the biosynthesis of vitamin B,, of very much higher specific activity than that obtainable using 6oCo.…”