Embryo culture media for human IVF: which possibilities exist?

Gruber, Irmhild; Klein, Matthias

doi:10.5152/jtgga.2011.25

Cited by 44 publications

(29 citation statements)

References 87 publications

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“…Different sequential media formulations have been reported to achieve acceptable rates of embryo development in vitro; however, there is usually limited information available on success rate following embryo transfer. Sequential culture strategies consider at least two phases covering pre-and-postcompaction stages, but, there remains much controversy regarding sequential vs. one-step culture with both similar and contradictory results [62].…”

Section: Preimplantation Embryo Metabolism and In Vitro Environmentmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

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Despite advantages of in vitro embryo production in many species, widespread use of this technology is limited by generally lower developmental competence of in vitro derived embryos compared to in vivo counterparts. Regardless, in vivo or in vitro gametes and embryos face and must adjust to multiple microenvironments especially at preimplantation stages. Moreover, the embryo has to be able to further adapt to environmental cues in utero to result in the birth of live and healthy offspring. Enormous strides have been made in understanding and meeting stage-specific requirements of preimplantation embryos, but interpretation of the data is made difficult due to the complexity of the wide array of culture systems and the remarkable plasticity of developing embryos that seem able to develop under a variety of conditions. Nevertheless, a primary objective remains meeting, as closely as possible, the preimplantation embryo requirements as provided in vivo. In general, oocytes and embryos develop more satisfactorily when cultured in groups. However, optimization of individual culture of oocytes and embryos is an important goal and area of intensive current research for both animal and human clinical application. Successful culture of individual embryos is of primary importance in order to avoid ovarian superstimulation and the associated physiological and psychological disadvantages for patients. This review emphasizes stage specific shifts in embryo metabolism and requirements and research to optimize in vitro embryo culture conditions and supplementation, with a view to optimizing embryo culture in general, and culture of single embryos in particular.

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Section: Preimplantation Embryo Metabolism and In Vitro Environmentmentioning

confidence: 99%

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Absalon-Medina

Butler

Gilbert

2014

J Assist Reprod Genet

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“…To provide an optimal environment for zygote/embryos, clinical embryologist has long been involved in optimizing the culture conditions. Previous work 1 – 3 has evaluated embryo metabolism, as well as its homeostasis, including focusing on the role of in vitro specific media components in order to determine what, and when, should be added in embryo culture medium. For example that pyruvate, as a powerful antioxidant, is able to reduce intracellular levels of hydrogen peroxide in the embryo 4 .…”

Section: Introductionmentioning

confidence: 99%

Common medium versus advanced IVF medium for cryopreserved oocytes in heterologous cycles

Poverini¹,

Lisi²,

Lisi³

et al. 2018

Cell Death Discovery

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Granulocyte-macrophage colony-stimulation factor plays different crucial roles during embryo implantation and subsequent development. Here we aimed to evaluate the effects of embryo cell culture medium, with the inclusion of granulocyte-macrophage colony-stimulation factor (GM-CSF), on embryo development and pregnancy rate. To this end, we took advantage of our retrospective observational study to correlate the outcomes from two different culture media. We included in this study 25 unselected patient from our IVF Center that underwent heterologous IVF cycle with crypreserved oocytes. We analyze the fertilization rate, pregnancy rate, and embryo quality at different day of transfer obtained from two different media composition. Our results show that the rate of fertilization and the pregnancy rate were increased using medium added with this particular type of cytokines (GM-CSF).

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“…Technologies have been improved for maintaining the temperature and the appropriate in vivo-like pH range [1]. Moreover, for several decades, researchers have been developing technologies to make culture media and additives that can facilitate germ cell development [2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Effect of micro-vibration culture system on embryo development

Hur

Park

Ryu

et al. 2013

J Assist Reprod Genet

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Purpose Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders. Materials and methods The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (n=178) and a micro-vibration culture group (n=181) in mice. The embryonic development rates and clinical results were compared between a static culture group (n=159 cycles) and a micro-vibration culture group (n=166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.Results The embryonic development rate was significantly improved in the micro-vibration culture group in mice (p<0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (p<0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (p<0.05). Conclusions Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.

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Embryo culture media for human IVF: which possibilities exist?

Cited by 44 publications

References 87 publications

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Preimplantation embryo metabolism and culture systems: experience from domestic animals and clinical implications

Common medium versus advanced IVF medium for cryopreserved oocytes in heterologous cycles

Effect of micro-vibration culture system on embryo development

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