Embryo culture of Costus speciosus (Koen.) Sm. to regenerate variable diosgenin yielding clones

Pal, Amita; Roy, Ashis Taru

doi:10.1007/bf00232512

Cited by 5 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some authors also suggested that cell differentiation status and morphogenesis changed SG production by plant cell cultures [ 58 ]. However despite the remarkable research interest no common pattern of SG formation in cell cultures in response to growth conditions has been found.…”

Section: Steroid Glycosides (Sg) In Cultures Of Undifferentiated Planmentioning

confidence: 98%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

View full text Add to dashboard Cite

Plant cell culture is traditionally viewed as a unique artifi cially created biological system representing a heterogenous population of dedifferentiated cells. This system undergoes a continuous process of autoselection based on the intensity and stability of cell proliferation. We discuss here the details of formation and regulation of isoprenoid biosynthesis in plant cell in vitro based on literature survey and our research. Obviously, secondary metabolism differs in cell culture compared to the plant per se , because in cell culture metabolites are synthesized and compartmentalized within a single heterotrophic cell with sparse or underdeveloped vacuoles and plastids. For example, in plant cell cultures isoprenoid biosynthesis via MVA pathway was found to be more active than via plastid-localized MEP pathway. Also, it was hypothesized that cell cultures preferably produce metabolites, which promote cell proliferation and growth. Indeed, cell cultures of Dioscorea deltoidea produced mainly furostanol glycosides, which promoted cell division. Triterpene glycosides (ginsenosides) in the cell cultures of various Panax species are represented mainly by Rg-and Rb-groups. Rb ginsenosides are predominantly found as malonyl-esters that may infl uence their intracellular localization.Despite the difference in the isoprenoid composition in plant and cell culture the latter became an attractive source of phytochemicals as an alternative to plant harvesting. We provide in this chapter the guidelines to biotechnological production of plant isoprenoids using plant cell cultures and discuss the optimal methods of bioreactor-based cultivation and cryopreservation of plant cell collections.

show abstract

Section: Steroid Glycosides (Sg) In Cultures Of Undifferentiated Planmentioning

confidence: 98%

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Носов

Попова

Кочкин

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

View full text Add to dashboard Cite

show abstract

“…Production of secondary metabolites from medicinal plants of Western Ghats through in vitro methodsSen et al 1992;Krombholz et al 1992 Coscinium fenestratum Callus culture, cell suspension Berberine JayakumaranNair et al 1992, Khan et al 2008 Diosgenin Jain et al 1984, Pal and Roy 1991 CamptothecinRoja and Heble 1994, Fulzele et al 2001, Thengane et al 2003, Sundravelan et al 2004. Yamamoto and Yamada 1986, Falkenhagen et al 1993, Benjamin et al 1993, Kirillova et al 2001, Roja and Heble 1996 AnthraquinonesSuzuki et al 1985Shin 1989, Radha et al 2010a …”

mentioning

confidence: 98%

Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology

Krishnan

Decruse

Radha

2011

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

Climate change, alien species, and use of land for intensive farming and development are causing severe threat to the plant genetic diversity worldwide. Hence, conservation of biodiversity is considered fundamental and also provides the livelihoods to millions of people worldwide. Medicinal plants play a key role in the treatment of a number of diseases, and they are only the source of medicine for majority of people in the developing world. The tropical regions of the world supply the bulk of current global demand for "natural medicine," albeit with increasing threat to populations in the world and its genetic diversity. India is a major center of origin and diversity of crop and medicinal plants. India poses out 20,000 species of higher plants, one third of it being endemic and 500 species are categorized to have medicinal value. The Western Ghats is one of the major repositories of medicinal plants. It harbors around 4,000 species of higher plants of which 450 species are threatened. Currently, the number of species added to the red list category in this region is increasing, and the valuable genetic resources are being lost at a rapid rate. Demand for medicinal plants is increasing, and this leads to unscrupulous collection from the wild and adulteration of supplies. Providing high-quality planting material for sustainable use and thereby saving the genetic diversity of plants in the wild is important. During the last 25 years of intensive research, Tropical Botanic Garden and Research Institute has developed in vitro protocol for rapid regeneration and establishment of about 40 medicinally important rare and threatened plants of Western Ghats.In situ conservation alone would not be effective in safeguarding these important species. Thus, utilizing the biotechnoligical approach to complement ex situ conservation program is becoming vital. Propagating biotechnology tools in plant conservation program is a prerequisite to succeed in sustainable use and to complement the existing ex situ measures. In addition to propagation, storage of these valuable genetic resources is equally important. In vitro slow growth of 35 species and cryopreservation using embryo/meristem/seed in 20 different species of rare medicinal plants of this region is accomplished. Plants developed in vitro of ten medicinal plants, which have restricted distribution, were reintroduced in the natural habitat as well.

show abstract

“…Several investigations are known as regards in vitro propagation studies conducted in medicinal plants (Table . 1). Regeneration via direct organogenesis Pal and Roy (1991) found that leaf, node, and internode as explants removed from C. speciosus were able to grow many elongated shoots by 4.44 µg BAP supplemented MS media. They also noticed that elongated shoots were efficiently rooted on a halfstrength MS medium.…”

Section: In Vitro Propagationmentioning

confidence: 99%

In vitro propagation of pharmaceutical and endangered medicinal plants- a mini review

Bharathi,

Prakash

2022

J. Current Opinion Crop Sci.,

View full text Add to dashboard Cite

Peoples and communities gain significantly from the use of medicinal herbs. The medicinal potential of these plants is related to the huge number of chemical compounds that have a specific physiological effect on the human body. Herbal drugs and phytonutrients, also known as nutraceuticals, are growing more popular around the world. The conventional strategy of propagation is the most popular and takes a very long time to multiply due to the low rate of fruit set, poor germination, and clonal homogeneity is not always preserved via seeds. Using optimal in vitro settings, elite plants may be rapidly multiplied to create pharmacognostic material delivering essential metabolites and also produce disease-free plants. This review summarises research on in vitro propagation methods established for medicinal plants, to use this technology to mass-produce medicinal plants that have been over-exploited.

show abstract

Embryo culture of Costus speciosus (Koen.) Sm. to regenerate variable diosgenin yielding clones

Cited by 5 publications

References 3 publications

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Isoprenoid Production via Plant Cell Cultures: Biosynthesis, Accumulation and Scaling-Up to Bioreactors

Conservation of medicinal plants of Western Ghats, India and its sustainable utilization through in vitro technology

In vitro propagation of pharmaceutical and endangered medicinal plants- a mini review

Contact Info

Product

Resources

About