Embryo density and medium volume effects on early murine embryo development

Canseco, R. S.; Sparks, Amy E.T.; Pearson, R.E.; Gwazdauskas, F.C.

doi:10.1007/bf01204051

Cited by 62 publications

(28 citation statements)

References 6 publications

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“…Although some reports indicated that mammalian embryos cultured in groups exhibit better embryonic development than those cultured singly [1][2][3][4], it remains unclear by what mechanism group culture promotes embryonic development in vitro. It has been postulated that embryos secrete certain growth factors as embryonic autocoids to support or promote their development in vitro [5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 98%

“…It has been postulated that embryos secrete certain growth factors as embryonic autocoids to support or promote their development in vitro [5][6][7][8][9][10]. Group culture with different numbers of embryos in different volumes of culture medium resulted in differential embryonic development in vitro [2,3,11]. Therefore, the volume of micro-drop (culture medium) or density of embryos is an important factor for embryonic development in vitro.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Effect of culture medium volume and embryo density on early mouse embryonic development: Tracking the development of the individual embryo

Dai¹,

Xu²,

Wang³

et al. 2012

J Assist Reprod Genet

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Effect of culture medium volume and embryo density on early mouse embryonic development: Tracking the development of the individual embryo

Dai¹,

Xu²,

Wang³

et al. 2012

J Assist Reprod Genet

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“…However, for commercial purposes such as use in OPU, where it is usually necessary just to keep the immature oocytes from one donor together, a small number of oocytes/embryos can be cultured (approximately three to six oocytes recovered per nonstimulated cow; Rizos et al, 2005;Chaubal et al, 2006). It has been widely reported that in vitro embryo development in bovine and other mammalian species tends to be suppressed in cultures with a single or low number of embryos (in mouse: Paria and Dey, 1990;Canseco et al, 1992;Lane and Gardner, 1992;Kato and Tsunoda, 1994;in bovine: Palma et al, 1992;Ferry et al, 1994;Keefer et al, 1994;Blondin and Sirard, 1995;Carolan et al, 1996;Donnay et al, 1997;O'Doherty et al, 1997;Ward et al, 2000;Pereira et al, 2005;Fujita et al, 2006). It seems that the culture systems developed to culture embryos in groups could be unsuitable and/or incomplete for individual embryo culture, and there must be some kind of limiting factor or condition in single embryo in vitro culture systems which remain still without knowing (Nagao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

Salvador

Cebrián-Serrano

Salamone

et al. 1970

Span. j. agric. res.

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The aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0%; 0% vs. 37.8%, respectively; p < 0.05). In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture (2.9; 10.2-10.8; 33.2% in single, low number of embryos (5-10), and controlgrouped, respectively; p < 0.05). In Experiment 5, it was observed that the last part of the culture period (day 3 onwards) seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number (5-10) of embryos culture.Additional key words: single/low embryo culture. ResumenEfecto del número de ovocitos y embriones en la maduración, fertilización y desarrollo embrionario in vitro en vacunoEl objetivo de este trabajo fue identificar la etapa del proceso de producción de embriones in vitro en la cual, el cultivo individualizado o con bajo número de oocitos/embriones (n = 5 ó 10) penaliza al desarrollo embrionario respecto del cultivo en grupo (n = 50). En el Experimento 1, se confirmó que la producción individualizada de embriones in vitro resulta en una menor tasa de división embrionaria y de blastocistos que las obtenidas con el cultivo en grupo (49,4 vs. 83,0%; 0% vs. 37,8%, respectivamente; p < 0,05). En los Experimentos 2 y 3, no se observó efecto sobre el desarrollo embrionario del cultivo individualizado o de bajo número de embriones en las etapas de maduración y fertilización, respectivamente. En el Experimento 4, se observó un efecto negativo sobre la tasa de blastocistos durante el cultivo in vitro post-inseminación cuando se cultivaron los embriones individualmente o con bajo número [2,9; 10,2-10,8; 33,2% en el cultivo individual, con bajo número de embriones (5-10), y grupo control (50), respectivamente; p < 0,05]. En el Experimento 5, se observó que en la última parte del cultivo (día 3 post-inseminación en adelante) es donde más parece que se manifiesta el efecto negativo del cultivo con número reducido de embriones. Como conclusión, el cultivo post-inseminación, especialmente entre los días 3 y 7 de cultivo, parece ser la etapa más determinante del proceso de producción de embriones in vitro para el desarrollo embrionario en el cultivo individualizado y/o en número reducido de embriones (5-10).Palabras clave adicionales: cultivo individualizado/bajo número de embriones.

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“…While cell types requiring longer attachment times may need a larger drop volume to prevent hypoxia, previous studies have shown that embryos cultured in a 5 l drop volume under silicone oil remain viable after 120 h. 21 Mineral oil was selected for all further cell-seeding experiments. Silicone oil was omitted due to its strong adsorption to exposed silanol groups on glass surfaces.…”

Section: B Cell Attachmentmentioning

confidence: 99%

A novel technique for positioning multiple cell types by liquid handling

2009

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The spatial control of cells on a surface and the patterning of multiple cell types is an important tool for fundamental biological research and tissue engineering applications. A novel technique is described for the controlled seeding of multiple cell types at specific locations on a surface without requiring the use of specialized equipment or materials. Small-volume, quasi-hemispherical drops of cell solution are deposited onto a cell culture surface immersed under barrier oil, which serves to contain the drop and prevents evaporation of the cell culture medium during the time necessary for cells to attach to the cell culture surface. Subsequent flooding with an aqueous cell-compatible buffer displaces the barrier oil, allowing the cells to grow freely across the surface. This technique offers a simple and easily implemented solution for defining the initial position of cultured cells. The coculture of multiple cell types may be carried out by incorporating different cell types in each drop. A suitable drop volume was found to be 1 l dispensed with a standard 0.5-10 l pipette. The drop formed resulted in a footprint diameter of approximately 2 mm. Mineral oil and silicone oil do not compromise the viability of cultured cells when used in this technique. Moreover, a surface with heparin-immobilized FGF2 is shown to retain its bioactivity following drying of the substrate and contact with mineral oil.

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Embryo density and medium volume effects on early murine embryo development

Abstract: Our results suggest that a stimulatory interaction occurs among embryos possibly exerted through the secretion of growth factors. This effect can be diluted if the embryos are cultured in large drops or singly.

Cited by 62 publications

References 6 publications

Effect of culture medium volume and embryo density on early mouse embryonic development: Tracking the development of the individual embryo

Effect of culture medium volume and embryo density on early mouse embryonic development: Tracking the development of the individual embryo

Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine

A novel technique for positioning multiple cell types by liquid handling

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