Embryo development and gestation using fresh and vitrified oocytes

Almodin, Carlos Gilberto; Minguetti-Câmara, Vania Cibele; Paixao, Cassia Lopes; Pereira, Priscila Cardoso

doi:10.1093/humrep/deq042

Cited by 86 publications

(66 citation statements)

References 27 publications

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“…Cobo et al 22 randomized 600 patients to receive either fresh donor oocytes or frozen oocytes post vitrification and found no difference in the pregnancy rate per transfer (55.4% vs. 55.6%) or ongoing pregnancy rate per transfer (49.1% vs. 48.3%). Those results have been replicated in multiple other retrospective studies [23][24][25] . Other authors have also commented on the lack of differences noted in embryo quality between fresh and frozen oocytes from the same donor 26,27 .…”

Section: Oocyte Cryopreservation With Ovarian Stimulationsupporting

confidence: 55%

Oncofertility in Canada: cryopreservation and alternative options for future parenthood

Ronn¹,

Holzer²

2013

Curr. Oncol.

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Section: Oocyte Cryopreservation With Ovarian Stimulationsupporting

confidence: 55%

Oncofertility in Canada: cryopreservation and alternative options for future parenthood

Ronn¹,

Holzer²

2013

Curr. Oncol.

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“…In the freeze-all group, good-quality embryos were cryopreserved on day 3 by vitrification using an open system as previously described [26,27]. Briefly, the vitrification procedure was performed by embryo exposure to the equilibrium solution, followed by a 30-s exposure to the vitrification solution.…”

Section: Cryopreservation/thawing and Endometrial Primingmentioning

confidence: 99%

Freeze-all cycle for all normal responders?

Roque

Valle

Guimarães

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this study is to evaluate the freeze-all strategy in subgroups of normal responders, to assess whether this strategy is beneficial regardless of ovarian response, and to evaluate the possibility of implementing an individualized embryo transfer (iET) based on ovarian response. Methods This was an observational, cohort study performed in a private IVF center. A total of 938 IVF cycles were included in this study. The patients were submitted to controlled ovarian stimulation (COS) with a gonadotropin-releasing hormone (GnRH) antagonist protocol and a cleavage-stage day 3 embryo transfer. We performed a comparison of outcomes between the fresh embryo transfer (n = 523) and the freezeall cycles (n = 415). The analysis was performed in two subgroups of patients based on the number of retrieved oocytes: Group 1 (4-9 oocytes) and Group 2 (10-15 oocytes). Result(s) In Group 1 (4-9 retrieved oocytes), the implantation rates (IR) were 17.9 and 20.5% (P = 0.259) in the fresh and freeze-all group, respectively; the ongoing pregnancy rates (OPR) were 31 and 33% (P = 0.577) in the fresh and freezeall group, respectively. In Group 2 (10-15 oocytes), the IR were 22.1 and 30.1% (P = 0.028) and the OPR were 34 and 47% (P = 0.021) in the fresh and freeze-all groups, respectively. Conclusion(s) Although the freeze-all policy may be related to better in vitro fertilization (IVF) outcomes in normal responders, these potential advantages decrease with worsening ovarian response. Patients with poorer ovarian response do not benefit from the freeze-all strategy.

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“…Fertilization was assessed 18-20 h after ICSI, and embryos were kept in culture until day three post-fertilization as previously reported (Almodin et al, 2010). Good quality, Grade I embryos were defined as those with 6-8 cells, similarly-shaped blastomeres and no fragmentation, while Grade II embryos as those with 6-8 cells and similarly-shaped blastomeres with ≤20% fragmentation (Alikhani et al, 1999).…”

Section: Oocyte Pickup Ivf Embryo Culture and Transfermentioning

confidence: 99%

“…The Vitri-Ingá method employed for oocyte vitrification was that described previously by Almodin et al, 2010. All materials required for vitrification/warming were obtained from Ingamed (Maringá, Brazil).…”

Section: Oocyte Vitrification and Warmingmentioning

confidence: 99%

“…Vitrification, a rapidly developing technology, which is increasingly being used for the cryopreservation of gametes, embryos (particularly blastocysts) and germinative tissue, has been demonstrated to be a reliable strategy for the cryopreservation of oocytes (Cobo et al, 2008;Cobo et al, 2010;Almodin et al, 2010). The latest systematic review and meta-analysis comparing vitrified with fresh oocytes demonstrated no statistically significant differences in fertilization, cleavage and clinical pregnancy rates, although ongoing pregnancy rate was reduced in the vitrified group (Potdar et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Vitrification of Human Oocytes and its Contribution to In Vitro Fertilization Programs

Almodin

Ceschin²,

Nakano

et al. 2015

JBRA Assisted Reproduction

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Objeticve: To study the cumulative pregnancy outcome, particularly in terms of live births, with the consecutive transfer of embryos from fresh and vitrified/warmed oocytes to infertile patients in a routine infertility program. Methods: Patients were initially submitted to in vitro fertilization embryo transfer with fresh embryos, while surplus oocytes were vitrified with the Vitri-Ingá method. Patients who did not succeed to carry their gestation to term underwent a new cycle with embryos from their own warmed oocytes. Some of the patients participating in the first warming cycle, who still possessed surplus oocytes, underwent a second warming cycle. Clinical and pregnancy outcomes obtained with fresh and warming cycles were compared using the chi-square test at a level of significance of 5%. Results: Of the 211 participating patients, 97 (46%) got pregnant with fresh embryo transfer, and 69 (32.7%) carried their pregnancies to term. Of the patients participating in the first and second warming cycles, 32/100 (32%) and 6/20 (30.0%) resulted in live births, respectively. Thus, of the 211 participating patients, 107 carried their pregnancies to term, representing a cumulative live birth rate of 50.7%. No statistically significant differences between the use fresh and vitrified oocytes were found for any of the variables studied. Conclusions: Oocyte vitrification offered the possibility of gestation in more than one attempt after just one controlled hyperstimulation. Apart from alleviating the financial burden on patients, vitrification of oocytes may result in a feasible solution for the problems generated by abandoned frozen embryos.

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Embryo development and gestation using fresh and vitrified oocytes

Abstract: Despite some differences in the patient groups, the clinical results of this study demonstrated that the Vitri-ingá method preserves the potential of human vitrified oocytes for fertilization and further development.

Cited by 86 publications

References 27 publications

Oncofertility in Canada: cryopreservation and alternative options for future parenthood

Oncofertility in Canada: cryopreservation and alternative options for future parenthood

Freeze-all cycle for all normal responders?

Vitrification of Human Oocytes and its Contribution to In Vitro Fertilization Programs

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