Embryo development in dynamic microfluidic systems

Greco, Gina; Agostini, Matteo; Barone, Stefano; Cecchini, Marco

doi:10.1016/j.snb.2017.04.186

Cited by 15 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The virtual immune cell trajectories were numerically visualized at varying initial displacements (Movie S2, Supporting Information). Notably, the maximum shear stress applied to the cells was computed as 46.7 dyne cm −2 at the bottom plane of the PDMS well (Figure S9, Supporting Information), [35] which is consistent with the previous observations for cellular alteration (i.e., Piezo1 activation and Ca 2+ influx). [36,37] The discrepancy for the 3D maximum flow velocities between the experimental and computational analysis can be attributed to inaccurate 2D PIV calculation and different dynamic viscosities of fluid.…”

Section: Characterization Of Asf In the Saw Microreactorsupporting

confidence: 89%

“…In addition, the particle tracing was conducted to simulate the 3D cell trajectories formed by the SAW‐induced ASF with the assumption of cells to be 14.1 µm diameter solid microspheres. To calculate the fluid shear stress applied to a cell by SAW‐derived flow, the shear stress (SS) was computationally analyzed by applying the following equation; SS = (3/2)·( μV / r ), [ 35 ] where μ is the fluid dynamic viscosity at 37 °C, r is the particle radius, and V is the velocity magnitude of computational analysis result at 600 pm initial displacement which is empirically chosen for high similarity with experiment.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Acoustofluidic Stimulation of Functional Immune Cells in a Microreactor

et al. 2022

View full text Add to dashboard Cite

The cytotoxic response of natural killer (NK) cells in a microreactor to surface acoustic waves (SAWs) is investigated, where the SAWs produce an acoustic streaming flow. The Rayleigh-type SAWs form by an interdigital transducer propagated along the surface of a piezoelectric substrate in order to allow the dynamic stimulation of functional immune cells in a noncontact and rotor-free manner. The developed acoustofluidic microreactor enables a dynamic cell culture to be set up in a miniaturized system while maintaining the performance of agitating media. The present SAW system creates acoustic streaming flow in the cylindrical microreactor and applies flow-induced shear stress to the cells. The suspended NK cells are found to not be damaged by the SAW operation of the adjusted experimental setup. Suspended NK cell aggregates subjected to an SAW treatment show increased intracellular Ca 2+ concentrations. Simultaneously treating the NK cells with SAWs and protein kinase C activator enhances the lysosomal protein expressions of the cells and the cell-mediated cytotoxicity against target tumor cells. These have important implications by showing that acoustically actuated system allows dynamic cell culture without cell damages and further alters cytotoxicity-related cellular activities.

show abstract

Section: Characterization Of Asf In the Saw Microreactorsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Acoustofluidic Stimulation of Functional Immune Cells in a Microreactor

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Moreover, the SAW chip is designed to allow the activation of different IDTs to generate SAWs along different directions, with the aim of testing new fluid-dynamics configurations. The dynamic in vitro environment created with this technology can be exploited to further improve organs-on-a-chip: it can allow better mimicking of the physiological dynamic environment that cells in our body typically experience. ,, Finally, our study further supports the great versatility and biocompatibility of the SAW technology for cell manipulation. ,,, …”

Section: Discussionsupporting

confidence: 61%

“…The SS applied by a fluid to a cell in suspension, assuming it to be a sphere of radius r ≈ 10 μm, is calculated as where μ is the dynamic viscosity of water at 37 °C, and v is the relative velocity.…”

Section: Experimental Sectionmentioning

confidence: 99%

Surface-Acoustic-Wave (SAW)-Driven Device for Dynamic Cell Cultures

et al. 2018

Self Cite

View full text Add to dashboard Cite

In the last few decades, new types of cell cultures have been introduced to provide better cell survival and development, with micro- and nanoenvironmental physicochemical conditions aimed at mimicking those present in vivo. However, despite the efforts made, the systems available to date are often difficult to replicate and use. Here, an easy-to-use surface-acoustic-wave (SAW)-based platform is presented for realizing dynamic cell cultures that is compatible with standard optical microscopes, incubators, and cell-culture dishes. The SAW chip is coupled to a standard Petri dish via a polydimethylsiloxane (PDMS) disc and consists of a lithium niobate (LN) substrate on which gold interdigital transducers (IDTs) are patterned to generate the SAWs and induce acoustic streaming in the dish. SAW excitation is verified and characterized by laser Doppler vibrometry, and the fluid dynamics is studied by microparticle image velocimetry (μPIV). Heating is measured by an infrared (IR) thermal camera. We finally tested this device with the U-937 monocyte cell line for viability and proliferation and cell-morphological analysis. The data demonstrate that it is possible to induce significant fluid recirculation within the Petri dish while maintaining negligible heating. Remarkably, cell proliferation in this condition was enhanced by 36 ± 12% with respect to those of standard static cultures. Finally, we show that cell death does not increase and that cell morphology is not altered in the presence of SAWs. This device is the first demonstration that SAW-induced streaming can mechanically improve cell proliferation and further supports the great versatility and biocompatibility of the SAW technology for cell manipulation.

show abstract

“…Fluid motion, mixing, droplet displacement, and atomization are all feasible with this technology [15]- [18], down to the nanoliter-scale [19], [20]. The compatibility of SAWs with cells has also been studied [21]- [23], as well as their enhancement of molecules binding to surfaces [24]- [26]. These SAW-based devices are fabricated by standard micro-and nano-fabrication techniques.…”

Section: Introductionmentioning

confidence: 99%