Embryo-induced alterations in the molecular phenotype of primate endometrium

Nimbkar-Joshi, Shruti; Rosario, Gracy X.; Katkam, R.R.; Manjramkar, Dhananjay D.; Metkari, Siddhanath; Puri, Chander P; Sachdeva, Geetanjali

doi:10.1016/j.jri.2009.08.011

Cited by 19 publications

(22 citation statements)

References 44 publications

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“…This finding indicates that the presence of embryos in the reproductive tract can prime the endometrium through unknown embryonic factors, leading to an expansion of the implantation window. A previous study employing non-human primates reports that super-imposition of endometrial receptivity on embryonic stimuli causes remarkable changes in endometrial expression of pro-inflammatory cytokines and immunosuppressive factors, which regulate endometrial transformation conducive for embryo survival, growth and development (Nimbkar-Joshi et al, 2009). A similar phenomenon has been observed in Lif null pregnant mice, in which implantation fails to occur, but the uterine luminal epithelium exhibits COX-2 expression at the apposition site of blastocysts (Fouladi-Nashta et al, 2005; Song et al, 2000), reinforcing the notion that embryo-derived signaling molecules are able to regulate uterine preparation for implantation.…”

Section: Embryonic Preparation For Implantation Necessitates “Blasmentioning

confidence: 99%

Physiological and molecular determinants of embryo implantation

Zhang

Lin

Kong

et al. 2013

Molecular Aspects of Medicine

450

480

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Embryo implantation involves the intimate interaction between an implantation-competent blastocyst and a receptive uterus, which occurs in a limited time period known as the window of implantation. Emerging evidence shows that defects originating during embryo implantation induce ripple effects with adverse consequences on later gestation events, highlighting the significance of this event for pregnancy success. Although a multitude of cellular events and molecular pathways involved in embryo-uterine crosstalk during implantation have been identified through gene expression studies and genetically engineered mouse models, a comprehensive understanding of the nature of embryo implantation is still missing. This review focuses on recent progress with particular attention to physiological and molecular determinants of blastocyst activation, uterine receptivity, blastocyst attachment and uterine decidualization. A better understanding of underlying mechanisms governing embryo implantation should generate new strategies to rectify implantation failure and improve pregnancy rates in women.

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Section: Embryonic Preparation For Implantation Necessitates “Blasmentioning

confidence: 99%

Physiological and molecular determinants of embryo implantation

Zhang

Lin

Kong

et al. 2013

Molecular Aspects of Medicine

450

480

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“…In humans, this lasts from approximately day 20 to day 24 of the menstrual cycle (Achache and Revel, 2006;Aplin, 2000;Aplin and Kimber, 2004;Bergh and Navot, 1992;Enders, 1989;Psychoyos, 1974). After the endometrium becomes receptive and the embryo reaches the blastocyst stage, the embryo must initiate interaction with the luminal epithelium (Dey, 2004;Nimbkar-Joshi et al, 2009;Rashid et al, 2011;Tabibzadeh et al, 1987). A cascade of physiological and molecular events is then triggered, leading to the establishment of a stable maternal-conceptus relationship (Garrido-Gomez et al, 2010;Simón et al, 2000a;Simón et al, 2000b).…”

Section: Introductionmentioning

confidence: 99%

miR-145 suppresses embryo–epithelial juxtacrine communication at implantation by modulating maternal IGF1R

Kang¹,

Lees²,

Matthews³

et al. 2015

Development

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Successful implantation requires the synchronization of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk between the embryo and the endometrium remain elusive; however, recent studies have revealed that there are alterations in endometrial microRNAs (miRs) in women suffering repeated implantation failure and that one of the altered miRs is miR-145. We assessed the role of miR-145 and its target IGF1R, in early implantation. miR-145 overexpression and IGF1R knockdown were achieved in Ishikawa endometrial cells. Quantitative PCR, western blotting and 39UTR luciferase reporter assays confirmed that IGF1R is a direct target of miR-145 in the endometrium. Attachment of mouse embryos or IGF1-coated beads to endometrial epithelial cells was used to study the effects of altered miR-145 and/or IGF1R expression on early implantation events. miR-145 overexpression or specific reduction of IGF1R impaired attachment in both cases. An IGF1R target protector prevented the miR-145-mediated reduction in IGF1R and reversed the effect of miR-145 overexpression on attachment. The data demonstrate that miR-145 influences embryo attachment by reducing the level of IGF1R in endometrium.

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“…For example, murine rodents undergo eccentric implantation, where the luminal epithelium forms implantation chambers by invagination surrounding the trophoblast (Dey et al, 2004;Reese et al, 2008). Conversely, primates undergo interstitial implantation, where the syncytial trophoblast formed near the inner cell mass adheres to the uterine epithelium and penetrates into the stroma by intruding between endometrial epithelial cells (Nimbkar-Joshi et al, 2009). In humans, specific interactions such as apposition and early embryo attachment to the uterine epithelium are difficult to observe, due to the tenuous and transient nature of these mechanisms, and investigations rely on biopsies obtained during the estimated time of embryo attachment, or at predicted times in the menstrual cycle (Klemmt et al, 2009;Sela et al, 2013).…”

Section: The Need To Develop Novel Research Methodologymentioning

confidence: 99%

“…While 25% of embryos implant successfully following in vitro fertilization (IVF), it is not yet possible to accurately determine the rate of implantation failure in humans, because we have yet to develop a reproducible method with which to evaluate the pre-implantation period (Diedrich et al, 2007;de Mouzon et al, 2010;Cha et al, 2012). Successful implantation of the embryo represents a fundamental biological interaction involving intricate molecular signaling between the implanting embryo and the host endometrium (Dey et al, 2004;Nimbkar-Joshi et al, 2009). Acquiring an enhanced understanding of the molecular signaling networks that coordinate strategies for successful implantation is critical to our basic knowledge of human reproduction, and may lead to novel approaches to improve the outcome of both natural pregnancies and pregnancy via assisted reproductive technology (ART).…”

Section: Introductionmentioning

confidence: 99%

Molecular mechanisms of membrane interaction at implantation

Davidson

Coward

2016

Birth Defects Research Pt C

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Successful pregnancy is dependent upon the implantation of a competent embryo into a receptive endometrium. Despite major advancement in our understanding of reproductive medicine over the last few decades, implantation failure still occurs in both normal pregnancies and those created artificially by assisted reproductive technology (ART). Consequently, there is significant interest in elucidating the etiology of implantation failure. The complex multistep process of implantation begins when the developing embryo first makes contact with the plasma membrane of epithelial cells within the uterine environment. However, although this biological interaction marks the beginning of a fundamental developmental process, our knowledge of the intricate physiological and molecular processes involved remains sparse. In this synopsis, we aim to provide an overview of our current understanding of the morphological changes which occur to the plasma membrane of the uterine endothelium, and the molecular mechanisms that control communication between the early embryo and the endometrium during implantation. A multitude of molecular factors have been implicated in this complex process, including endometrial integrins, extracellular matrix molecules, adhesion molecules, growth factors, and ion channels. We also explore the development of in vitro models for embryo implantation to help researchers investigate mechanisms which may underlie implantation failure. Understanding the precise molecular pathways associated with implantation failure could help us to generate new prognostic/diagnostic biomarkers, and may identify novel therapeutic targets.

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Embryo-induced alterations in the molecular phenotype of primate endometrium

Cited by 19 publications

References 44 publications

Physiological and molecular determinants of embryo implantation

Physiological and molecular determinants of embryo implantation

miR-145 suppresses embryo–epithelial juxtacrine communication at implantation by modulating maternal IGF1R

Molecular mechanisms of membrane interaction at implantation

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