Embryo of Flowering Plants at the Critical Stage of Embryogenesis Relative Autonomy (by Example of Cereals)

Круглова, Н.Н.; Titova, G. E.; Seldimirova, O.A.; Zinatullina, А.Е.; Веселов, Д. С.

doi:10.1134/s1062360420010026

Cited by 7 publications

(3 citation statements)

References 91 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Проблема критических стадий эмбриогенеза цветковых растений и критериев их выявления в литературе поставлена достаточно давно (история вопроса подробно изложена в работах [32,35]). В частности, Т.Б.…”

unclassified

Embryo Culture in Vitro as a Biotechnological Method: Capabilities and Limits (On the Cereals Example)

ZINATULLINA

2023

Proceedings of the RAS Ufa Scientific Centre

View full text Add to dashboard Cite

The review article considers the use of the embryo culture in vitro method, which consists in the culture in vitro of zygotic embryos of different ages, in modern biotechnological studies of cereals, using the example of the literature and own data. Special attention is paid to the analysis of immature embryos culture in vitro as more effective explants in comparison with mature embryos, with a possible explanation for this effectiveness. The analysis of the cereal embryogenesis periodization and the identification of the critical stage of the relative autonomy of immature embryos on its basis is given. The method for obtaining regenerants from immature embryos under in vitro and ex vitro conditions through the stage of morphogenic callus formation, as well as the method for directly obtaining regenerants by passing the stage of callus formation, are discussed. Examples of successful application of the method in the elaboration of techniques to increase the adaptation of immature embryos/morphogenic callus/regenerants to the stress factor of drought are given. Both the possibilities and limitations of embryo culture in vitro as a biotechnological method are evaluated. The principle of universality of plant morphogenesis processes in natural and experimental conditions, put forward by T. B. Batygina (2014 and earlier), is confirmed.

show abstract

unclassified

Embryo Culture in Vitro as a Biotechnological Method: Capabilities and Limits (On the Cereals Example)

ZINATULLINA

2023

Proceedings of the RAS Ufa Scientific Centre

View full text Add to dashboard Cite

show abstract

“…При оценке экспланта -зрелого или незрелого зародыша злаков -исследователи, как правило, не указывают, на какой стадии эмбриогенеза находится эксплант в момент инокуляции на индукционную питательную среду. В то же время, по мнению ряда авторов [14,15], стадия развития инокулируемого зародыша in planta -один из важнейших факторов формирования регенерантов в культуре in vitro. Для выявления оптимальной для применения в биотехнологических исследованиях стадии развития зародыша необходимо применение унифицированных периодизаций эмбриогенеза злаков, основанных на данных классической эмбриологии злаков.…”

unclassified

“…У злаков формирование растений-регенерантов в эмбриокультуре in vitro протекает успешнее при использовании в качестве исходных эксплантов незрелых зародышей -в сравнении со зрелыми ( [13][14][15] В качестве «точки отсчета» в периодизации эмбриогенеза злаков исследователь принимает зиготу -клетку, образующуюся в резуль-тате сингамии -слияния гаплоидных гамет (женской гаметы-яйцеклетки и мужской гаметы-спермия). Автор полагает, что зигота -это инициальная клетка зародыша и начальная фаза онтогенеза амфимиктически размножающихся растений.…”

unclassified

Periodizations of Cereal Zygotic Embryogenesis in Planta and It’s Use in Biotechnological Research in Vitro

Zinatullina¹

2022

Proceedings of the RAS Ufa Scientific Centre

View full text Add to dashboard Cite

In modern biotechnological studies of commercially valuable cereals, promising areas of great interest are the embryo culture and callus culture in vitro, associated with the use of zygotic embryos of different ages that form full-fledged regenerated plants. These biotechnologies are largely based on the use of the morphogenetic potential of embryonic cells, mainly immature ones. At the same time, the progress of such biotechnologies is limited by the lack of a single unified periodization of cereal zygotic embryogenesis in planta. The difficulty of creating a single periodization is due to both the specifics of the course of cereal embryogenesis according to a special Graminad-type and the morphology of the mature embryo, which is characterized by unique organs – the scutellum, coleoptile, epiblast, mesocotylt, coleorhiza. The article provides a brief review of the works devoted to the identification of the structural features of cereal embryos in the dynamics of development in natural conditions from the zygote to the mature structure. Special attention is paid to the comparative analysis of the proposed periodizations of zygotic embryogenesis in planta, especially those used in biotechnological studies in vitro of members of this family. It is emphasized that biotechnological and other experimental studies using cereal embryos should be based on the data of classical descriptive plant embryology.

show abstract

Cytophysiological Features of the Cereal-Based Experimental System “Embryo In Vivo–Callus In Vitro”

et al. 2021

View full text Add to dashboard Cite

Embryo of Flowering Plants at the Critical Stage of Embryogenesis Relative Autonomy (by Example of Cereals)

Cited by 7 publications

References 91 publications

Embryo Culture in Vitro as a Biotechnological Method: Capabilities and Limits (On the Cereals Example)

Embryo Culture in Vitro as a Biotechnological Method: Capabilities and Limits (On the Cereals Example)

Periodizations of Cereal Zygotic Embryogenesis in Planta and It’s Use in Biotechnological Research in Vitro

Cytophysiological Features of the Cereal-Based Experimental System “Embryo In Vivo–Callus In Vitro”

Contact Info

Product

Resources

About