Embryo production and recovery in goats by non-surgical transcervical technique

The aim of this research was to evaluate the effectiveness of three pFSH doses (80 mg; 145 mg and 215 mg) on ovarian response and on quantity and quality of transferable embryos of goats during the breeding and the non-breeding seasons. Ovary structures were exposed (laparatomy under general anaesthesia) and numbers of follicles and corpora lutea were registered. Surgical embryo flushing was conducted to count and classify embryos. There were more follicles (3.4 ± 1.1) in does administered 80 mg of pFSH (p < 0.05) than in goats administered 145 mg of pFSH (2.2 ± 1.1) and 215 mg of pFSH (0.9 ± 0.6). Numbers of corpora lutea, blastocysts, and recovered and transferable embryos of goats administered 145 mg pFSH (13.4 ± 3.7, 2.42 ± 1.0, 3.4 ± 1.2 and 3.2 ± 1.1, respectively) and those of goats administered 215 mg pFSH (11.6 ± 2.6, 3.2 ± 0.9, 5.7 ± 1.5, and 5.6 ± 1.5) were greater (p < 0.05) than values obtained from goats administered 80 mg pFSH (4.0 ± 1.5, 0.5 ± 0.3, 1.0 ± 0.5, and 0.8 ± 0.5). Numbers of morula of does administered 80 and 145 mg pFSH (0.4 ± 0.4 and 0.8 ± 0.3) were lower (p < 0.05) than those obtained from animals treated with 215 mg pFSH (2.4 ± 0.9). There was no effect of season upon the analyzed variables. In conclusion, under the prevalent conditions in north-eastern Mexico, administration of 145 or 215 mg pFSH in a decreasing dose schedule over 3.5 days to bred goats provided a satisfactory superovulatory result.

Section: Discussionsupporting

confidence: 93%

“…; Fonseca et al. ), as also increasing doses of pFSH led to better ovarian response in terms of number of corpora lutea and transferable embryos (Goel and Agrawal ; Iheukwumere et al. ).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Three pFSH Doses on Superovulation and Embryo Quality in Goats During Two Breeding Seasons in North‐eastern Mexico

Sánchez-Dávila

Ledezma-Torres

Padilla‐Rivas

et al. 2014

“…As occurred previously in bovine species (Castro Neto et al, ), many efforts are now being made to eliminate surgical procedures from MOET programmes in small ruminants (Fonseca et al, ). In most sheep MOET programmes, the standard embryo collection method is surgical, even when the nonsurgical collection is scheduled, and surgical collection is programmed by default if the TC collection becomes infeasible.…”

Section: Discussionmentioning

confidence: 99%

Cervical transposition test using Hegar dilator at oestrus as a tool to select ewes for transcervical embryo collection

Santos

Arashiro

Balaro

et al. 2018

This study evaluated the cervical transposition method as a tool to select ewes for embryo collection by transcervical route. Adult Santa Inês ewes (n = 50) received Day 0 protocol for superovulation treatments. The cervix transposition test was performed both at oestrus and at the embryo collection time. The latter was preceded by hormonal cervical dilation. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 85.7%, 66.6%, 85.7%, 66.6% and 80.0%, respectively. The kappa index yielded a moderate score (κ = 0.52). In conclusion, the high sensitivity and accuracy indicate that the cervical transposition test is a screening option to select ewes for embryo collection by transcervical route.

“…; Sohnrey and Holtz ; Fonseca et al. ), but others reported that only 61% of goat cervices were penetrable (Lima‐Verde et al. ).…”

Section: Discussionmentioning

confidence: 99%

Successful Use of Endoscopy for Transcervical Cannulation Procedures in the Goat

Colagross-Schouten¹,

Allison²,

Brent³

et al. 2014

ContentsTwo methods for transcervical cannulation of the goat were evaluated during a contraception study in 15 adult female Nigerian dwarf and African pygmy goats. Twenty-four transcervical cannulation procedures were conducted in which seven females underwent the procedure 2–3 times. Initially, a rigid 4-mm stainless steel cannula and external light source were used in 19 procedures to introduce the contraceptive compound into the uterus. Placement of the cannula was directed by feel or depth assessment. Of seven females that were euthanized following this procedure, four evidenced complications including penetration of the cervix with the cannula and cervical damage. A 2-mm custom-made endoscope with a specially designed cannula was then used for the remaining five procedures. No complications were found. A single animal, that underwent the endoscopic procedure twice, was euthanized for study purposes and no abnormal findings of the reproductive tract were reported. The use of an endoscope resulted in better outcomes because the uterus could be visualized after traversing the cervix.