Embryo quality before and after slow freezing: Viability, implantation and pregnancy rates in 627 single frozen-thawed embryo replacement cycles following failure of fresh transfer

Capodanno, Francesco; Feo, Gaetano De; Gizzo, Salvatore; Nicoli, Alessia; Palomba, Stefano; Sala, Giovanni Battista La

doi:10.1016/j.repbio.2016.03.002

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…A Chinese group [12] supported the ongoing culture of poor cleavage-stage embryos: 569 poor-quality embryos cultured in vitro formed 248 blastocysts, of which 138 were high-quality (24.25%). In agreement with the Chinese group, in 2016 Capodanno et al [13] summarized the outcome of surplus cleavage-stage embryos that were thawed and transferred: the poor-grade embryos demonstrated the same implantation potential when compared to those with high scores. Nevertheless, when implantation occurred, embryo quality reflected a correlation to the chance for an ongoing pregnancy.…”

Section: Discussionmentioning

confidence: 56%

What we learned from extended culture of ‘rejected’ day-3 cleavage stage embryos: a prospective cohort study

et al. 2017

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BackgroundTo test whether poor quality day-3 embryos can undergo successful blastulation and implantation.MethodsA prospective cohort study was conducted. Whether or not a good quality embryo was transferred on day-3, poor quality (rejected) embryos were further cultured and followed. The clinical outcome of each embryo was assessed.ResultsA total of 694 rejected embryos (from 205 patients) were included, with a blastulation rate of 21.2% (147 embryos) compared to 64.2% general blastulation rate reported by our laboratory (P < 0.01). In a multivariate logistic regression model, only their grade on day-3 significantly affected blastulation (P = 0.01). A total of 97 embryos attained eligibility for fresh transfer or cryopreservation, only 6 of which resulted from a day-3 embryo scored < 2. Of these, 52 were transferred, resulting in 21 pregnancies (16 clinical and 5 chemical). In summary, 694 cultured embryos yielded 16 clinical pregnancies; a 2.3% clinical pregnancy rate.ConclusionsLow score day-3 embryos can result in successful blastulation and clinical pregnancies. However, the normal blastulation rate is poor.

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Section: Discussionmentioning

confidence: 56%

What we learned from extended culture of ‘rejected’ day-3 cleavage stage embryos: a prospective cohort study

et al. 2017

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“…However, other studies supported that FET cycles could lead to cryo-injury which may influence the genetic potential of embryos and blastomeres. Degenerated blastomeres may influence the embryos implantation [25]. Therefore, the duration of supraphysiological hormonal levels and embryo freezing time on subsequent outcomes deserved our attention and enhance physician treatment confidence who hesitated whether start FET cycle immediately.…”

Section: Discussionmentioning

confidence: 99%

“…As we all know, frozen-thawed procedures could lead to cryoinjury. Capodanno et al [25] supported that cryo-injury could influence the genetic potential of embryos and blastomeres. Therefore, we suspected whether delayed FET cycles may increase embryo freezing and in vitro time which eventually increased the risk of macrosomia.…”

Section: Discussionmentioning

confidence: 99%

Pregnancy and perinatal outcomes in pregnancies resulting from time interval between a freeze-all cycle and a subsequent frozen-thawed single blastocyst transfer

Long

et al. 2020

BMC Pregnancy Childbirth

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Background Adverse obstetric outcomes are correlated with altered circulating hormone levels at the time implantation by the trophectoderm. What’ more, embryo freezing process may also have adverse effect on perinatal outcomes. This study aims to evaluate whether increasing interval time between a freeze-all cycle and a subsequent frozen-thawed single blastocyst transfer could have any effect on pregnancy and perinatal outcomes. Methods This was a retrospective cohort study included the first single blastocyst transfer in artificially cycles of all patients who underwent a freeze-all cycle between January 1st, 2016 and September 30th, 2018. All patients were divided into two groups according to the time interval between oocyte retrieval and the day of first frozen-thawed embryo transferred (FET): Group 1 (immediate FET cycles) and Group 2 (delayed FET cycles). Results No significant differences were reported between the two groups regarding the rates of clinical pregnancy, live birth, biochemical pregnancy and pregnancy loss even after adjusting for measured confounding. When accounting for perinatal outcomes, gestational age, birth weight, delivery mode, fetus gender, preterm birth, gestational hypertension, GDM, placenta previa, fetal malformation and low birthweight also did not vary significantly between the two groups. Only the incidence of macrosomia was more frequently in the Group 2 compared with the Group 1 (AOR 3.886, 95%CI 1.153–13.103, P = 0.029) after adjusting with a multiple logistic regression model. Conclusions We found delayed FET cycles for blastocyst transfer following freeze-all cycles may not improve the pregnancy outcomes. On the contrary, postponement of FET cycles may increase the risk of macrosomia. Therefore, FET cycles for blastocyst transfer should be done immediately to avoid adverse effects of delayed time on perinatal outcomes.

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Impact of cell loss after warming of human vitrified day 3 embryos on obstetric outcome in single frozen embryo transfers

Guardo

Racca

Coticchio³

et al. 2022

J Assist Reprod Genet

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Embryo quality before and after slow freezing: Viability, implantation and pregnancy rates in 627 single frozen-thawed embryo replacement cycles following failure of fresh transfer

Cited by 6 publications

References 31 publications

What we learned from extended culture of ‘rejected’ day-3 cleavage stage embryos: a prospective cohort study

What we learned from extended culture of ‘rejected’ day-3 cleavage stage embryos: a prospective cohort study

Pregnancy and perinatal outcomes in pregnancies resulting from time interval between a freeze-all cycle and a subsequent frozen-thawed single blastocyst transfer

Impact of cell loss after warming of human vitrified day 3 embryos on obstetric outcome in single frozen embryo transfers

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