Embryo recovery results in Hispano-Arabe horse and Spanish donkey breeds

Marı́n, C.; Vizuete, Guillermo; Galisteo, J.

doi:10.1016/j.livsci.2017.10.011

Cited by 9 publications

(16 citation statements)

References 35 publications

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“…In this line, numerous studies have been conducted to examine the factors that affect embryo recovery, quality, and diameter in mares [13,22,29]. However, in donkeys, these studies have been very scarce [17,20], and they are often performed on a limited number of animals, cycles, or embryos. Therefore, more studies are needed to optimize embryo recovery rates and maximize the success of future ET programs in donkeys.…”

Section: Discussionmentioning

confidence: 99%

“…In donkeys, only a few studies have been conducted, and the results could not prove the influence of embryo quality and age on embryo recovery rate [16,17]. Similarly, no effect on embryo recovery rate and quality was observed by Pérez-Marín et al [20] The aim of the present study was to determine which factors affect the recovery rate, morphological quality, and diameter in embryos from Andalusian donkey jennies as a prerequisite to improve the success of both embryo transfer and cryopreservation in this endangered donkey breed.…”

Section: Introductionmentioning

confidence: 90%

“…Together with this technique, the cryopreservation of embryos and their storage in embryo banks offer several advantages to the preservation and management of equine endangered species [13,14]. However, in donkeys, the studies on both procedures are scarce and recent [15][16][17][18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies

Dorado

Bottrel

Ortiz

et al. 2020

Animals

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Embryo transfer and the vitrification of embryos could be used for the conservation and recovery of endangered donkey breeds. It is important to develop techniques that optimize recovery rates and the cryotolerance of donkey embryos. This study evaluates factors affecting the recovery rate, quality, and diameter of embryos obtained from donor jennies as a starting point for the use of vitrification and embryo transfer in the conservation of the Andalusian donkey. A total of 100 embryos were recovered out of 124 estrous cycles (80.6%). The donor jenny affected the rates of positive flushings (PFR; p = 0.040) and embryo recovery (ERR; p < 0.05) as well as embryo quality (p = 0.004). ERR was also affected by the number of flushings (p < 0.001), donor age (p < 0.05), successive cycle within donor (p < 0.001), and jacks (p < 0.05). Number of flushings (p < 0.001) and jack (p < 0.05) had a significant effect on PFR, whereas the day of flushing influenced the developmental stage (p < 0.001), embryo quality (p < 0.05), and diameter of embryos (p < 0.001). The number of flushings significantly influenced the diameter (p = 0.038) and embryo developmental stage (p = 0.001), whereas the developmental stage was statistically different between herds (p = 0.020). The factors influencing the success of this assisted reproductive technique were donor jenny, donor age, successive cycle within donor, day of flushing, number of flushings, and jack. The identification of these key points is crucial to achieve a higher efficiency of embryo transfer and vitrification processes, before considering their application in the conservation of endangered donkey breeds.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 90%

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Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies

Dorado

Bottrel

Ortiz

et al. 2020

Animals

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“…Donkey's population recovery is important due to the roles they play in the economy of emerging contries (8) and the production of hybrid animals like mules (9) . The estrous cycle of jennies and mares differs mainly in the duration of the heat period reflected in a longer estrous length in jennies (10) ; however the time lapse between the ovulation-fertilization relative to embryo entrance into the uterus seem to be similar between jennies (11,12,13) and mares (14,15) . The size of the embryo recovered either during the sixth, seventh, eighth or ninth day post ovulation (PO) varies dramatically; both, in jennies (12,13,16,17) and mares (15,16,18) ; however, it follows a similar trend in size increase.…”

Section: Introductionmentioning

confidence: 99%

“…The PO day selected to proceed with the intrauterine infusion of solutions to collect embryos in jennies or mares, determines their development stage (morula, blastocyst or expanded blastocyst), and will dictate the protocol suitable to optimize their PVPR (12,13,15,(19)(20)(21)(22) . By infusing the uterus on the sixth day PO, the recovered embryo will generally be smaller than 300 micrometers (12,16,21) ; on the other hand, embryos collected the eighth or ninth day PO will be equal or larger than 300 micrometers in size (12,13,20,23,24) . The optimization of embryo vitrification protocols requires a precise determination of the ovulation's day (day zero) to accurately determine the age/stage of embryo, since the ones collected during the sixth day PO do not require size reduction; while embryoss collected the seventh, eighth, or ninth need to be reduced in size previous to vitrification (12,21,25,26,27) .…”

Section: Introductionmentioning

confidence: 99%

Tasa de preñez post vitrificación en los embriones de équidos producidos in vivo. Revisión

Urías-Castro

Boeta

2020

Rev. Mex. Cienc. Pecu.

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Vitrification is a cryo-preservation method often used in embryos obtained from mares or jennies. It consists in the dramatic reduction of temperature to levels close to -196 °C, that allows the cryopreserving solution containing the embryo to pass from liquid to vitreous state. Several improvements to vitrification protocols have made possible to cryo-preserve embryos with different sizes; since during the first decade after the year 2000, only small embryos were successfully vitrified. Embryos collected at the sixth day post ovulation (PO) are usually smaller or equal to 300 micrometers in diameter (≤ 300 µmØ) and can be routinely vitrified following simple protocols; they have a higher post vitrification pregnancy rate (PVPR) when compared to large embryos which have more than 300 micrometers in diameter (˃ 300 µmØ). The high PVPR of embryos ≤ 300 µmØ is due to an embryo capsule (EC) that is not fully developed yet and has a high permeability to cryo-preserving solutions. At present time, embryos collected either the seventh or eighth day PO are ˃ 300 µmØ and are characterized to have a low post vitrification survival; in order to increase their PVPR their EC might be punctured to make it permeable to cryopreserving solutions. Additionally, there are at least two factors that can be manipulated to increase the PVPR of embryos ˃ 300 µmØ; one is to reduce their size by aspiring their blastocoelic liquid (BL), and the other is to induce a high temperature transfer index (TTI) to rapidly reach -196 °C.

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Comparative Efficacy of Histrelin Acetate and hCG for Inducing Ovulation in Brazilian Northeastern Jennies (Equus africanus asinus)

Oliveira

Segabinazzi

Canuto

et al. 2020

Journal of Equine Veterinary Science

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Embryo recovery results in Hispano-Arabe horse and Spanish donkey breeds

Cited by 9 publications

References 35 publications

Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies

Factors Affecting Embryo Recovery Rate, Quality, and Diameter in Andalusian Donkey Jennies

Tasa de preñez post vitrificación en los embriones de équidos producidos in vivo. Revisión

Comparative Efficacy of Histrelin Acetate and hCG for Inducing Ovulation in Brazilian Northeastern Jennies (Equus africanus asinus)

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