Embryo splitting

Illmensee, Karl; Levanduski, Mike

doi:10.1016/j.mefs.2010.05.001

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…The published data, especially from studies on human embryos, are controversial. Several recent studies have suggested that the embryo splitting technique can be a potential method for production of viable and morphologically adequate blastocysts (17,18,24). However, they did not present qualitative assessments of blastocysts created using in vitro splitting.…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of the in vitro splitting of human preimplantation embryos from ART programs

Omidi¹,

Khalili²,

Agha‐Rahimi³

et al. 2021

Turk J Med Sci

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Background/aim: The aim was to evaluate the efficiency of IES procedures; we also assessed the quality of the blastocysts developed from embryos obtained from different sources. Materials and Methods: Good quality embryos at 6-8 cell stages were categorized according to their fertilization sources: 1) frozen-warmed donated embryos, 2) chromosomally abnormal embryos, 3) parthenogenetic embryos and 4) embryos derived from fertilization of in vitro matured oocytes (rescue IVM). After IES, splitting and developmental efficiency was assessed. Furthermore, the quality of the developed blastocysts was evaluated by Hoechst and propidium iodide (PI) staining. Results: The data showed a high rate of both splitting and developmental efficiency in the frozen-warmed embryos after IES (140% and 71.7%, respectively), followed by chromosomally abnormal embryos (96.8% and 52.5%, respectively). Results of the Hoechst and PI staining showed that the mean± SD cell numbers of control group was higher (113.11±16.01) than that of twins A (donor blastomeres embryos, 58±12.2) and twins B (recipient blastomeres embryos, 50.4±8.5), respectively. Conclusion: The chromosomally normal embryos enrolled in IES are more potent to develop to the viable blastocyst. For research purposes, 1PN and 3PN embryos are one the best option for splitting procedures, regardless of the poor quality of developed blastocysts. Key words: Human embryo, In vitro splitting, Blastocyst

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Section: Discussionmentioning

confidence: 99%

“…In clinical approaches, the numerical increasing of embryos is beneficial for patients enrolled in IVF programs, especially for women at advanced ages and patients considered as "poor responders" (20,24,25). A very limited number of studies have been conducted on in vitro splitting in humans and also in chromosomally normal embryos.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the in vitro splitting of human preimplantation embryos from ART programs

Omidi¹,

Khalili²,

Agha‐Rahimi³

et al. 2021

Turk J Med Sci

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“…Pemisahan embrio dilakukan pada saat embrio berada dalam fase blastomere. Pemisahan dapat dilakukan satu sampai empat kali, tergantung pada species ternak dan kondisi grade embrio (Tabel 1) (Karl Illmensee, 2010). Namun peningkatan jumlah blastomer yang diperoleh tidak berbanding lurus dengan peningkatan jumlah blastokista yang layak untuk ditransfer.…”

Section: B Pengertian Kloning Dan Metode Kloningunclassified

Bioteknologi Teori dan Aplikasi

Syam¹,

Kurniati²

2022

Preprint

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Ketergantungan yang tinggi terhadap sumber energi fosil dipandang sebagai masalah fundamental sistem energi global maupun nasional. Produksi sumber energi fosil tidak terbarukan dipastikab akan menurun dan habis pada suatu saat sehingga tidak dapat diandalkan. Ketergantungan tinggi pada sumber energi fosil akan menyebabkan ancaman pada ketersediaan energi sebagai salah satu pilar utama ketahanan energi global maupun nasional. Data PBB menunjukkan bahwa pangsa sumber energi fosil (batubara, minyak bumi, gas bumi) cenderung menenurun dari 83,5 persen pada 1990 menjadi 82,3 persen pada tahun. Penurunan peran energi fosil diisi oleh biofuel dan sampah yang meningkat dari 7,7 persen pada 1990 menjadi 9,2 persen pada 2015. Bauran energi Indonesia pun sangat didominasi oleh bahan bakar fosil. Dominasi bahan bakar fosil dalam bauran energi Indonesia pada 2015 mencapai 95 persen yang berarti lebih tinggi daripada dalam bauran energi global. Kebijakan energi nasional sebagaimana dijabarkan dalam Rencana Umum Energi Nasional (RUEN, 2017) telah menetapkan untuk meningkatkan peran energi terbarukan paling sedikit 23 persen pada 2025 dan meningkat menjadi 31 persen pada 2050.

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“…In cattle, embryos split into blastomeres at the 4-cell stage could further develop to term giving rise to multiple monozygotic healthy calves [16].Splitting of embryo provides cheap identical twins for embryo sexing, detection of genetic abnormalities and genetic marker-based selection of embryos. Human embryo splitting has been reported recently [17]. However, the complex ethical, spiritual and legislative concerns should be closely observed when dealing with human embryo splitting [14].…”

Section: Splitting Of Embryomentioning

confidence: 99%

In Vitro Production of Embryos: Principles, Techniques and Applications

Adam¹

2016

International Journal of Research Studies in Biosciences

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Embryo splitting

Cited by 10 publications

References 35 publications

Efficacy of the in vitro splitting of human preimplantation embryos from ART programs

Efficacy of the in vitro splitting of human preimplantation embryos from ART programs

Bioteknologi Teori dan Aplikasi

In Vitro Production of Embryos: Principles, Techniques and Applications

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