Embryo transfer as an option to improve fertility in repeat breeder dairy cows

Nowicki, Arkadiusz

doi:10.2478/jvetres-2021-0018

Cited by 9 publications

(5 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The procedure appeared not to influence fertility, and the pregnancy rate was improved in RB cows. In fact, improvement of fertility in RB dairy cows by ET has been extensively reported (Nowicki, 2021). In this situation, our results reinforce previous findings that showed similar results when comparing the use of frozen IVP embryos and AI (Ambrose et al, 1999; Drost et al, 1999).…”

Section: Discussionmentioning

confidence: 98%

Direct transfer of a single frozen–thawed in vitro‐produced embryo to avoid twin pregnancy in dairy cows

López‐Gatius¹,

Palacín-Chauri

García-Ispierto

2022

Reprod Domestic Animals

View full text Add to dashboard Cite

Fertility of cow receiving fixed‐time artificial insemination (FTAI) was compared with cow in which a single frozen–thawed in vitro‐produced (IVP) embryo was fixed‐time transferred (FTET) to avoid twin pregnancy. The study population was comprised of 596 lactating dairy cows synchronized for oestrus: 440 were fixed‐time inseminated (AI cows), and 156 were given GnRH treatment at the time of embryo transfer (ET cows) 8 days post‐oestrus. Of the 596 cows, 235 (39.4%) became pregnant: 175 (39.8%) AI cows and 60 (39.8%) ET cows. Twin pregnancy was recorded in 16% of the AI pregnant cows (28/175), whereas no ET cows had twins (0/60). Significant interaction (p < .01) was observed between breeding technique (FTAI vs FTET) and repeat (RB) or not repeat breeding (NRB) for the likelihood of pregnancy. This meant that using RB AI cows as reference, the odds ratio for pregnancy in RB ET cows was 2.2 (p = .04). In conclusion, transfer of a frozen IVP embryo proved useful to prevent the risk of twin pregnancy without affecting fertility.

show abstract

Section: Discussionmentioning

confidence: 98%

Direct transfer of a single frozen–thawed in vitro‐produced embryo to avoid twin pregnancy in dairy cows

López‐Gatius¹,

Palacín-Chauri

García-Ispierto

2022

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…This substance bound to anterior pituitary gland cell receptors (Bekenov et al 2019) This causes a short-term racing sex hormones level in the blood serum. The increased gonadotropin content persists for 3-4h after injection (Mee et al 1993;Nowicki 2021).…”

Section: Figmentioning

confidence: 98%

The Dairy Cows’ Fertility Improvement

2023

Int J Vet Sci

View full text Add to dashboard Cite

Modern cattle farming needs to find ways to increase the fertility of dairy cows. That's why we had the goal to study the application of different schemes for stimulating increased fertility in cows. For investigation of the possibility of increasing the fertility of cows, three groups of animals were formed: control and two experimental groups, i.e., The first stimulation scheme included Surfagon (15μg) 1-3h before insemination, Catosal, Gabivit-Se, 10-15min before insemination, and Uteroton. Progesterone (2.5%), Catosal, and Gabivit-Se were injected on the 8th day after insemination. Animals of the second stimulated group received Ovariovit 30-60min before insemination. At the 25-30 th inseminated day, were injected Ovariovit and Liarsin. Experimental results were compared by the data of the control group (without stimulation using). Common diagnostics of the animal's wellness was conducted by clinical and laboratory studies. And the pregnancy was additionally diagnosed by a portable ultrasound scanner AcuVista RS880b and MyLabOneVET with a rectal linear probe. It was found using a complex insemination stimulation scheme aimed at preventing infertility in cows can reduce the service period to 85.60±1.93 days and the insemination index to 1.9±0.25 days. Homeopathic preparations using made it possible to achieve a service-period of 89.50±2.42 days and an insemination index of 2.0±0.27. At the same time, animal hematology study results before and after the experiment in the compare with the Control Group demonstrated the safety of the proposed schemes because of the constant of the main blood parameters. So, the introduction of the proposed stimulation schemes can shorten the service period and raise fecundation that have a great impact on the economic efficiency of the cattle breeding.

show abstract

“…Fertility in cattle has decreased dramatically in recent decades and is currently one of the major challenges in the dairy industry [1]. Assisted reproductive technologies (ART) play a crucial role in addressing low fertility and have been widely used in cattle worldwide to enhance the reproduction of animals with high genetic value [2,3]. Moreover, bovine in vitro embryo production (IVEP) enables the use of sub-fertile donors and repeat breeders.…”

Section: Introductionmentioning

confidence: 99%

Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy

Tsopp,

Kilk,

Taalberg

et al. 2024

Metabolites

View full text Add to dashboard Cite

This study investigated whether metabolomic fingerprints of bovine embryo growth media improve the prediction of successful embryo implantation. In this prospective cohort study, the metabolome from in vitro-produced day 7 blastocysts with successful implantation (n = 11), blastocysts with failed implantation (n = 10), and plain culture media without embryos (n = 5) were included. Samples were analyzed using an AbsoluteIDQ® p180 Targeted Metabolomics Kit with LC-MS/MS, and a total of 189 metabolites were analyzed from each sample. Blastocysts that resulted in successful embryo implantation had significantly higher levels of methionine sulfoxide (p < 0.001), DOPA (p < 0.05), spermidine (p < 0.001), acetylcarnitine-to-free-carnitine ratio (p < 0.05), C2 + C3-to-free-carnitine ratio (p < 0.05), and lower levels of threonine (nep < 0.001) and phosphatidylcholine PC ae C30:0 (p < 0.001) compared to control media. However, when compared to embryos that failed to implant, only DOPA, spermidine, C2/C0, (C2 + C3)/C0, and PC ae C30:0 levels differentiated significantly. In summary, our study identifies a panel of differential metabolites in the culture media of bovine blastocysts that could act as potential biomarkers for the selection of viable blastocysts before embryo transfer.

show abstract

Embryo transfer as an option to improve fertility in repeat breeder dairy cows

Cited by 9 publications

References 65 publications

Direct transfer of a single frozen–thawed in vitro‐produced embryo to avoid twin pregnancy in dairy cows

Direct transfer of a single frozen–thawed in vitro‐produced embryo to avoid twin pregnancy in dairy cows

The Dairy Cows’ Fertility Improvement

Associations of the Single Bovine Embryo Growth Media Metabolome with Successful Pregnancy

Contact Info

Product

Resources

About