Embryo transfer in dromedary camels

McKinnon, AO; Tinson, Alex; Nation, G.

doi:10.1016/s0093-691x(05)80060-3

Cited by 57 publications

(20 citation statements)

References 4 publications

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“…Previous studies in horses (Allen 1982a, Douglas 1982McCue and Troedsson 2003;Stout 2003;Wilsher and Allen 2004) and camels (McKinnon et al 1994;Skidmore et al 2002) achieved highest pregnancy rates when the recipient ovulated 0-2 days after, not before, the donor. However, the results of the present study have demonstrated that good pregnancy rates can also be achieved using asynchronous recipient mares that have ovulated up to 3 days before the donor, provided they are treated with meclofenamic acid.…”

Section: Discussionmentioning

confidence: 82%

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Wilsher¹,

Kölling²,

Allen³

2010

Equine Veterinary Journal

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Summary Reasons for performing study: A level of synchrony between embryo and uterine environment is essential for the establishment of pregnancy when performing embryo transfer. The ability to extend the acceptable degree of asynchrony would allow more efficient use of recipient mares. Objectives: To establish if administration of the prostaglandin synthetase inhibitor, meclofenamic acid, to asynchronous recipient mares could widen the acceptable window of asynchrony for embryo transfer. Hypothesis: The prostaglandin synthetase inhibitory action of meclofenamic acid may act to suppress luteolysis and thereby allow for a greater degree of asynchrony between donor and recipient mares. Methods: A total of 72 Grade 1 horse embryos were transferred nonsurgically into the uteri of recipient mares that had ovulated 2 (n = 20), 3 (n = 20), 4 (n = 16) or 5 (n = 16) days before the donor. Half of the mares in each group were treated orally with 1 g meclofenamic acid, beginning on Day 9 after ovulation and continuing for 7 days after embryo transfer. Results: Comparison of recipient:donor asynchrony between treated and untreated mares was: +2 days, 9/10 pregnancies vs. 8/10 (P = 1.00); by +3 days, 8/10 vs. 2/10 (P = 0.025); by +4 days, 5/8 vs. 1/8 (P = 0.121); and by +5 days 3/8 vs. 0/8 (P = 0.20). In 10/11 meclofenamic acid‐treated and 23/25 untreated recipient mares that failed to become pregnant, luteolysis occurred at the normal time (14–19 days) after ovulation. Conclusion: Treatment with meclofenamic acid supported the establishment of pregnancy in recipient mares that ovulated before the donors. However, meclofenamic acid appeared to provide this support in a manner other than by suppression of luteolysis. Potential relevance: Pregnancy rates can be increased in recipient mares that ovulate 3 days before the donor by administration of meclofenamic acid.

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Section: Discussionmentioning

confidence: 82%

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Wilsher¹,

Kölling²,

Allen³

2010

Equine Veterinary Journal

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“…1994). This hormone (eCG) is usually used alone (Dromedary: McKinnon et al. 1994; llamas: Ratto et al.…”

Section: Discussionmentioning

confidence: 99%

Quality and Developmental Ability of Dromedary (Camelus dromedarius) Embryos Obtained by IVM/IVF, In Vivo Matured/IVF or In Vivo Matured/Fertilized Oocytes

Khatir¹,

Anouassi

Tibary

2007

Reprod Domestic Animals

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The effect of source of cumulus-oocytes-complexes (COCs), maturation and fertilization conditions on developmental competence of dromedary embryos was examined. Thirty-six adult females were superovulated with equine Chorionic Gonadotropin (eCG) injection (3500 IU, IM) and divided in three groups of 12 females each. Group 1 provided 138 COC's collected from follicles >or= 5 mm 10 days after stimulation prior hCG treatment and matured in vitro for 30 h. Group 2 provided 120 in vivo matured oocytes which were aspirated from their follicles 20 h after hCG (3000 IU, IV) given on day 10 follow eCG injection. Group 3 provided 65 in vivo matured/fertilized oocytes. Females in Group 3 received hCG on day 10 following eCG treatment and then were mated 24 h later. Fertilized oocytes were collected from the oviducts of females 48-h post-mating. Quality of the oocytes was assessed after in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) of COCs. All cultures were performed in three replicates (n = 3) at 38.5 degrees C, under 5% CO(2) and high humidity (>95%). Only COCs with cumulus and homogenous (dark) cytoplasm were used. Nuclear maturation rate for Groups 1 and 2 was determined by epifluorescence microscopy in a sample of COCs (n = 30) denuded, fixed and stained with Hoechst 33342. To study the viability of obtained embryos, hatched blastocysts from each group were transferred to recipients followed by pregnancy diagnosis using ultrasonography at 15, 60 and 90 days. The percentage of COCs reaching metaphase II (MII) after 30 h of maturation was slightly but not significantly higher for in vivo matured oocytes (28/30; 93%) than those in vitro matured (25/30; 84%). The total rate of cleavage (2 cells to blastocyst stage) was not different for the three groups. However, significantly (p < 0.05) more blastocyst and hatched blastocysts were obtained from in vivo matured and in vivo fertilized oocytes (Group 3; 52% and 73%) than from in vitro fertilized oocytes whether they were matured in vitro (Group 1; 35% and 32%) or in vivo (Group 2; 32% and 45%). Pregnancy rates were not significantly different amongst all groups for the three first months following embryo transfer. All pregnancies were lost after day 90 follow transfer except for in vivo matured and in vivo matured/fertilized groups. Only in vivo matured/in vitro fertilized and in vivo matured/fertilized produced embryos continued normal development until term and resulted in the birth of normal and healthy live calves. Six claves (29%; 6/21) were born from Group 3 and one (8%; 1/13) calf was born from Group 2. This study shows that the IVC system used is able to support camel embryo development. However, developmental competence and viability of dromedary embryos may be directly related to the intrinsic quality (cytoplasmic maturation) of oocytes.

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“…Studies conducted in our laboratory on camels and alpacas show that the development of the embryo from fertilization to the hatched blastocyst stage takes about 6 to 6.5 days following ovulation and fertilization (day 7.5 after mating). The embryo reaches the uterus around this time, at the hatched expanding blastocyst stage Bourke et al, 1992;McKinnon et al, 1992;Picha et al, 2013;Tibary, 2010;Tibary et al, 2007). There does not seem to be a selective transport through the uterotubual junction (UTJ) as seen in the mare.…”

Section: Early Embryonic Development Within the Uterine Tubementioning

confidence: 99%