Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferons as adjuvants

Rautenschlein, Silke; Sharma, J. M.; Winslow, Barbara J.; McMillen, Janis; Junker, David; Cochran, Mark D.

doi:10.1016/s0264-410x(99)00254-6

Cited by 55 publications

(17 citation statements)

References 24 publications

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“…IFN c has been reported to be involved in macrophage activation and inhibition of virus replication [8]. A protective role of IFN c against NDV infection has also been demonstrated [26]. In this study the expression ratio of IFN c gene was constant up to 48 h, but was observed at least 16-folds more at 72 hpi than at 24 hpi.…”

Section: Discussionsupporting

confidence: 49%

Transcriptional response of chicken embryo cells to Newcastle disease virus (D58 strain) infection

Kumar¹,

Kirubaharan²,

Chandran³

et al. 2013

Indian J. Virol.

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Newcastle disease virus (NDV), the causative agent of Newcastle disease (ND) in chicken causes significant economic loss for the poultry industry worldwide. The mechanism involved in host response to NDV infection is not well understood. For better understanding of the virus-host interaction; transcriptional profile of some genes of chicken embryo (CE) cells infected with NDV vaccine strain D58 was established using quantitative RT-PCR SYBR Green method. The relative standard curve method was used to measure the level of transcripts of the cellular genes against an endogenous control (b actin) gene. Among the genes studied, IFN a, IFN c, MHC I and DDX 1 were up-regulated while IL 6 was down regulated. The expression of viral genes (M and F) in the infected CE cells was also confirmed by relative quantification. The host cellular genes involved in pro-inflammatory response, interferon-regulated proteins and the cellular immune response were affected by NDV infection, indicating involvement of complex signaling pathways of host cell responses to the infection. Thus, this study contributes to the understanding of the pathogenesis of ND and provides an insight into the virus-host interaction.

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Section: Discussionsupporting

confidence: 49%

Transcriptional response of chicken embryo cells to Newcastle disease virus (D58 strain) infection

Kumar¹,

Kirubaharan²,

Chandran³

et al. 2013

Indian J. Virol.

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“…(150) The vaccine efficacy of a recombinant fowlpox virus coexpressing a Newcastle disease virus (NDV) antigen and IFN-g was evaluated in turkeys. (153) The antibody response after in ovo vaccination developed earlier, and a larger proportion of birds was protected from challenge with NDV when the IFNg gene was present in the vector. Interestingly, chickens immunized with a recombinant fowlpox virus coexpressing ChIFN-a showed no enhanced immunity.…”

Section: Cytokines As Vaccine Adjuvantsmentioning

confidence: 99%

Cytokines of Birds: Conserved Functions—A Largely Different Look

Staeheli

Puehler

Schneider

et al. 2001

Journal of Interferon & Cytokine Research

126

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Targeted disruptions of the mouse genes for cytokines, cytokine receptors, or components of cytokine signaling cascades convincingly revealed the important roles of these molecules in immunologic processes. Cytokines are used at present as drugs to fight chronic microbial infections and cancer in humans, and they are being evaluated as immune response modifiers to improve vaccines. Until recently, only a few avian cytokines have been characterized, and potential applications thus have remained limited to mammals. Classic approaches to identify cytokine genes in birds proved difficult because sequence conservation is generally low. As new technology and high throughput sequencing became available, this situation changed quickly. We review here recent work that led to the identification of genes for the avian homologs of interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma, various interleukins (IL), and several chemokines. From the initial data on the biochemical properties of these molecules, a picture is emerging that shows that avian and mammalian cytokines may perform similar tasks, although their primary structures in most cases are remarkably different.

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“…Karaca et al (1998) described a recombinant fowlpox virus coexpressing chicken type I IFN and Newcastle disease virus hemagglutinin-neuraminidase and fusion genes that offered protective efficacy and humoral responses of chickens following in ovo or posthatch administration of infectious virus. Rautenschlein et al (1999) reported protection against Newcastle disease virus following embryo vaccination of turkeys with recombinant fowl pox virus constructs containing type I or type II IFN DNA adjuvants. A major research focus of our laboratory has been the identification of chicken cytokines as immunomodulators of Eimeria sp.…”

Section: Discussionmentioning

confidence: 99%

Embryo Vaccination Against Eimeria Tenella and E. Acervulina Infections Using Recombinant Proteins and Cytokine Adjuvants

Lillehoj

Ding

Dalloul

et al. 2005

Journal of Parasitology

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Avian coccidiosis is an intestinal disease caused by protozoa of the genus Eimeria. To investigate the potential of recombinant protein vaccines to control coccidiosis, we cloned 2 Eimeria sp. genes (EtMIC2 and 3-1E), expressed and purified their encoded proteins, and determined the efficacy of in ovo immunization to protect against Eimeria infections. Immunogen-specific serum antibody titers, parasite fecal shedding, and body weight gains were measured as parameters of disease. When administered alone, the recombinant EtMIC2 gene product induced significantly higher antibody responses, lower oocyst fecal shedding, and increased weight gains compared with nonvaccinated controls following infection with E. tenella. Combined embryo immunization with the EtMIC2 protein plus chicken cytokine or chemokine genes demonstrated that all 3 parameters of vaccination were improved compared with those of EtMIC2 alone. In particular, covaccination with EtMIC2 plus interleukin (IL)-8, IL-16, transforming growth factor-beta4, or lymphotactin significantly decreased oocyst shedding and improved weight gains beyond those achieved by EtMIC2 alone. Finally, individual vaccination with either EtMIC2 or 3-1E stimulated protection against infection by the heterologous parasite E. acervulina. Taken together, these results indicate that in ovo vaccination with the EtMIC2 protein plus cytokine/chemokine genes may be an effective method to control coccidiosis.

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Embryo vaccination of turkeys against Newcastle disease infection with recombinant fowlpox virus constructs containing interferons as adjuvants

Cited by 55 publications

References 24 publications

Transcriptional response of chicken embryo cells to Newcastle disease virus (D58 strain) infection

Transcriptional response of chicken embryo cells to Newcastle disease virus (D58 strain) infection

Cytokines of Birds: Conserved Functions—A Largely Different Look

Embryo Vaccination Against Eimeria Tenella and E. Acervulina Infections Using Recombinant Proteins and Cytokine Adjuvants

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