Embryogenic suspensions of adult cork oak: the first step towards mass propagation

Jiménez, J.; López‐Vela, Dolores; Ruiz-Galea, Mar; Celestino, C.; Toribio, M.; Alegre, Julio

doi:10.1007/s00468-012-0763-y

Cited by 9 publications

(3 citation statements)

References 33 publications

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“…Moreover, proliferation of Q. robur, Q. ilex and Q. bicolor embryogenic lines has also been significantly improved by applying this procedure (unpublished results). The high embryo productivity from nodular structures suggests the feasibility of developing methods for embryogenic cell suspension cultures, as well as the use of bioreactors for higher production rates and automation (Jiménez et al 2013). Our repetitive embryogenic system also provides a tool for studying the regulation of embryo development, and will benefit large-scale vegetative propagation efforts and germplasm resource conservation of elite Q. alba and Q. rubra cultures.…”

Section: Discussionmentioning

confidence: 99%

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Martínez

Viéitez

Corredoira

2015

Plant Cell Tiss Organ Cult

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In somatic embryogenic systems of various oak species, including Quercus alba and Q. rubra, embryogenic lines generally proliferate through generation of nodular embryogenic structures (NS) as a form of repetitive embryogenesis, without embryo development progressing to the cotyledonary stage, or with a low proportion of embryo formation, resulting in the inability to achieve suitable material for plant recovery. In experiments we used previously initiated embryogenic lines derived from 7-year-old trees of Q. alba and Q. rubra. The aim of this work was to improve embryo proliferation and histodifferentiation of lines from these oak species to obtain welldeveloped embryos and plant regeneration. The effects of activated charcoal, silver thiosulphate (STS) and sucrose concentration on secondary embryo production were investigated. Overall, the best embryo proliferation medium consisted of basal Murashige and Skoog medium containing 0.4 % charcoal and 20 lM STS, and supplemented with 6 % sucrose for Q. alba and 3 % sucrose for Q. rubra. In these conditions, well-developed and singularised torpedo-and cotyledonary-shaped embryos were produced. The developmental stage of the embryogenic explant used for subculture significantly influenced embryo production and differentiation of new secondary embryos, with NS being the most effective explant despite being the smallest explant type in terms of size and fresh weight.Addition of STS in the germination medium also had a positive effect on germination response giving rise to approximately 39 % embryo conversion in Q. alba and 11 % in Q. rubra.

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Section: Discussionmentioning

confidence: 99%

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Martínez

Viéitez

Corredoira

2015

Plant Cell Tiss Organ Cult

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“…Studies on the induction of primary somatic embryogenesis in cork oak and other forest species are numerous [22] [23] [24] [25]. On the other hand, secondary somatic embryogenesis has not been sufficiently studied.…”

Section: Introductionmentioning

confidence: 99%

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Rahmouni¹,

Ansari²,

Badoc³

et al. 2020

AJPS

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In the present study, we tested the effect of amino acids on secondary somatic embryogenesis of Moroccan cork oak (Quercus suber L.). Secondary mature and immature somatic embryos were obtained from primary somatic embryos cultured in N 30 K medium supplemented with nineteen amino acids. Stimulation of embryogenesis was dependent on the type and concentration of amino acid in the medium. Thus, L-glutamine and L-asparagine at 3.42 mM have been proved to be the most favorable for the formation of functional somatic embryos and the induction of secondary somatic embryos.

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“…Therefore, they are essential for mass propagation. Based on a protocol for establishing cork oak suspensions [20], we initiated liquid cultures from embryogenic lines of holm oak (unpublished results).…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of embryogenic tissues from mature holm oak trees

Barra-Jiménez¹,

Aronen²,

Alegre³

et al. 2015

Cryobiology

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Embryogenic suspensions of adult cork oak: the first step towards mass propagation

Cited by 9 publications

References 33 publications

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree

Cryopreservation of embryogenic tissues from mature holm oak trees

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Embryogenic suspensions of adult cork oak: the first step towards mass propagation

Cited by 9 publications

References 33 publications

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Improved secondary embryo production in Quercus alba and Q. rubra by activated charcoal, silver thiosulphate and sucrose: influence of embryogenic explant used for subculture

Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (&lt;i&gt;Quercus suber&lt;/i&gt; L.) Tree

Cryopreservation of embryogenic tissues from mature holm oak trees

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Effect of Amino Acids on Secondary Somatic Embryogenesis of Moroccan Cork Oak (<i>Quercus suber</i> L.) Tree