Embryoid bodies formation from chicken primordial germ cells

Xiong, Hui; Yun, Pu; Qing-yun, HU; Shan, Zhiqiang; Hu, Pengfei; Guan, Weijun; Ma, Yue

doi:10.1080/19768354.2014.921644

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…Embryoid body formation ability was assessed to prove the differentiation potential of PGCs. For a detailed description of this method see Xiong et al (2015). The germ layer specific genes AFP, GATA6 and Sox3 were detected by RT-PCR (primer information is shown in Table 1).…”

Section: Differentiation Potential Of Pgcsmentioning

confidence: 99%

“…Initiating the spermatogenesis from PGCs in vitro has been reported (Adams and McLaren, 2002;Matoba and Ogura, 2011), but the motile sperm had not been obtained. In our previous study, we have established a PGCs cell line and induced this sort of stem cell to differentiate into cardiomyocytes (Xiong et al, 2015), while in this work, motile sperm were derived from PGCs in a culture system outside the testicular environment, and the induced sperm characterised and identified. It is the first description of in vitro harvesting motile sperm in an avian species.…”

Section: Introductionmentioning

confidence: 99%

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Chicken Model for Inducing Primordial Germ Cells Differentiating into Motile Sperms in vitro

Xiong

Qing-yun

et al. 2017

Avian Biology Research

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Stem cell technologies have been widely used in the study of spermatogenesis. However, deriving motile sperm from stem cells in vitro is still rarely achieved. We found that chicken primordial germ cells could directly differentiate into sperm by using retinoic acid in a non-testicular culture system. The induced sperms were characterised by RT-PCR, immunofluorescence and flow cytometry techniques. Results suggested that chicken primordial germ cells could produce motile sperm in vitro. Our work has provided a novel animal model of spermatogenesis in vitro, which might be used for male reproductive mechanism research.

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Section: Differentiation Potential Of Pgcsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chicken Model for Inducing Primordial Germ Cells Differentiating into Motile Sperms in vitro

Xiong

Qing-yun

et al. 2017

Avian Biology Research

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The constitutively active pSMAD2/3 relatively improves the proliferation of chicken primordial germ cells

Zare

Mirhoseini

Ghovvati

et al. 2023

Molecular Reproduction Devel

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In many multicellular organisms, mature gametes originate from primordial germ cells (PGCs). Improvements in the culture of PGCs are important not only for developmental biology research, but also for preserving endangered species, and for genome editing and transgenic animal technologies. SMAD2/3 appear to be powerful regulators of gene expression; however, their potential positive impact on the regulation of PGC proliferation has not been taken into consideration. Here, the effect of TGF-β signaling as the upstream activator of SMAD2/3 transcription factors was evaluated on chicken PGCs' proliferation. For this, chicken PGCs at stages 26−28 Hamburger−Hamilton were obtained from the embryonic gonadal regions and cultured on different feeders or feeder-free substrates. The results showed that TGF-β signaling agonists (IDE1 and Activin-A) improved PGC proliferation to some extent while treatment with SB431542, the antagonist of TGF-β, disrupted PGCs' proliferation. However, the transfection of PGCs with constitutively active SMAD2/3 (SMAD2/3CA) resulted in improved PGC proliferation for more than 5 weeks. The results confirmed the interactions between overexpressed SMAD2/3CA and pluripotency-associated genes NANOG, OCT4, and SOX2. According to the results, the application of SMAD2/3CA could represent a step toward achieving an efficient expansion of avian PGCs.

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Creating a novel method for chicken primordial germ cell health monitoring using the fluorescent ubiquitination-based cell cycle indicator reporter system

Ecker,

Lázár,

Tóth

et al. 2024

Poultry Science

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Embryoid bodies formation from chicken primordial germ cells

Cited by 4 publications

References 22 publications

Chicken Model for Inducing Primordial Germ Cells Differentiating into Motile Sperms in vitro

Chicken Model for Inducing Primordial Germ Cells Differentiating into Motile Sperms in vitro

The constitutively active pSMAD2/3 relatively improves the proliferation of chicken primordial germ cells

Creating a novel method for chicken primordial germ cell health monitoring using the fluorescent ubiquitination-based cell cycle indicator reporter system

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