Embryoid body morphology influences diffusive transport of inductive biochemicals: A strategy for stem cell differentiation

Sachlos, Eleftherios; Auguste, Debra T.

doi:10.1016/j.biomaterials.2008.08.012

Cited by 106 publications

(103 citation statements)

References 42 publications

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“…The core composed necrotic ES cells and the surface composed proliferating ES cells. The surface was denser than the core, supporting the idea that the EB surface may have acted as a thick protective barrier that inadvertently restricted nutrient and gaseous permeation (Choi et al 2005;Sachlos and Auguste 2008). The outer and inner shells combine to represent the diffusion gradient of nutrient and gaseous exchange depicting transition between ES cell states at the surface and core (Jiang et al 2005).…”

Section: Discussionmentioning

confidence: 76%

“…EB formation was found to be a complex relationship between ES cell aggregation, proliferation, death, aggregation time and cluster agglomeration. Engineering could provide means to manipulate EB formation affecting EB size, number and morphology, and constitutive ES cell number and viability, all of which have major impact on downstream ES cell differentiation Messana et al 2008;Sachlos and Auguste 2008).…”

Section: Discussionmentioning

confidence: 99%

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Controlled embryoid body formation via surface modification and avidin–biotin cross-linking

et al. 2009

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Cell-cell interaction is an integral part of embryoid body (EB) formation controlling 3D aggregation. Manipulation of embryonic stem (ES) cell interactions could provide control over EB formation. Studies have shown a direct relationship between EB formation and ES cell differentiation. We have previously described a cell surface modification and cross-linking method for influencing cell-cell interaction and formation of multicellular constructs. Here we show further characterisation of this engineered aggregation. We demonstrate that engineering accelerates ES cell aggregation, forming larger, denser and more stable EBs than control samples, with no significant decrease in constituent ES cell viability. However, extended culture C5 days reveals significant core necrosis creating a layered EB structure. Accelerated aggregation through engineering circumvents this problem as EB formation time is reduced. We conclude that the proposed engineering method influences initial ES cell-ES cell interactions and EB formation. This methodology could be employed to further our understanding of intrinsic EB properties and their effect on ES cell differentiation.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Controlled embryoid body formation via surface modification and avidin–biotin cross-linking

et al. 2009

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“…This observation was in agreement with the previous reports. As described by Sachlos and Auguste (2008), during EBs' development, a fibrous ECM mainly consisting of collagen type I is secreted on the surface of EB and continues to form until the voids between cells are filled. By day 14, ECM deposition results in a smoother surface topography and the cell boundaries become indistinguishable.…”

Section: Resultsmentioning

confidence: 99%

Electron microscopic study of mouse embryonic stem cell-derived cardiomyocytes

et al. 2011

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“…E-cadherin is highly expressed in undifferentiated pluripotent cells as well as in the epiblastlike layers of cells in PSC-derived embryoid bodies (EBs). 26,27 E-cadherin is coupled to the cell actin-myosin cytoskeleton through a protein complex that includes p120-catenin, b-catenin, a-catenin, vinculin, and nonmuscle myosin IIA (NMMIIA). While both mESCs and hESCs express E-cadherin, they differ from one another in their dependence on intact cellcell signaling for survival.…”

Section: Mechanical Properties Of Hpscsmentioning

confidence: 99%

Mechanobiology of Human Pluripotent Stem Cells

Earls

Jin

2013

Tissue Engineering Part B: Reviews

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Human pluripotent stem cells (hPSCs) are self-renewing and have the potential to differentiate into any cell type in the body, making them attractive cell sources for applications in tissue engineering and regenerative medicine. However, in order for hPSCs to find use in the clinic, the mechanisms underlying their self-renewal and lineage commitment must be better understood. Many technologies that have been developed for the maintenance and directed differentiation of hPSCs involve the use of soluble growth factors, but recent studies suggest that other elements of the hPSC microenvironment also influence the growth and differentiation of hPSCs. This includes the influences of cell-cell interactions, substrate mechanics, cellular interactions with extracellular matrix, as well as the nanotopography of the substrate and physical forces such as shear stress, cyclic mechanical strain, and compression. In this review, we highlight the recent progress of this area of research and discuss ways in which the mechanical cues may be incorporated into hPSC culture regimes to improve methods for expanding and differentiating hPSCs.

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Embryoid body morphology influences diffusive transport of inductive biochemicals: A strategy for stem cell differentiation

Cited by 106 publications

References 42 publications

Controlled embryoid body formation via surface modification and avidin–biotin cross-linking

Controlled embryoid body formation via surface modification and avidin–biotin cross-linking

Electron microscopic study of mouse embryonic stem cell-derived cardiomyocytes

Mechanobiology of Human Pluripotent Stem Cells

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