Embryonal erythropoiesis and aging exploit ferroptosis

BRCA1 haploinsufficiency promotes chromosomal amplification under Fenton reaction-based carcinogenesis through ferroptosis-resistance

“…Refer to text for details. (D) Immunohistochemistry by HNEJ-1 monoclonal antibody [ 29 ] reveals significantly lower positivity in the kidney at 3 weeks of the Fe-NTA-induced renal carcinogenesis protocol in the Brca1 MUT(L63X/+) rats than WT, indicating that BRCA1 haploinsufficiency more efficiently generates ferroptosis-resistance in response to iron catalyzed oxidative stress (n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001 vs wild-type Fe-NTA at 3 weeks; bar = 200 μm; 50 μm in the inset in A, C and D; 100 μm; 50 μm in the inset in B). …”

“…HNEJ-1 antibody has been recently established as a tool to detect ferroptosis [ 29 ]. Whereas the immunostaining was not different between WT and MUT in the untreated control kidney, WT showed significantly more intense immunostaining than MUT at 3 weeks of carcinogenesis protocol ( Fig.…”

“…Precise renal tubular mechanism needs further investigation. However, a recently established ferroptosis marker HNEJ-1 immunostaining [ 29 ] as well as Cox-2 and cytoplasmic catalytic Fe(II) was significantly lower in the MUT model after 3-week Fe-NTA administration, indicating ferroptosis-resistance, whereas nuclear 8-OHdG level [ 31 ] was significantly higher in the MUT model. These fit well with previous findings that lipophilic antioxidants, such as vitamin E, was preventive in this renal carcinogenesis model [ 56 , 57 ].…”

“…Immunostainings were performed by BOND MAX/III (Leica, Wetzlar, Germany) with BOND Polymer Refine Detection (ds9800; Leica) as described [ 29 ]. Quantitation of immunohistochemical analysis, including Ki67 as a cell proliferation marker [ 30 ], was performed as described [ 31 ].…”

Targeting ferroptosis as a vulnerability in pulmonary diseases

“…Therefore, it is an effective approach to verify lipid peroxidation and ferroptosis by utilizing probes (C11-BODIPY 581/591) to directly detect oxidized PUFA-PLs [ 5 ]. Moreover, reactive toxic aldehydes among lipid peroxidation products such as 4-hydroxy-2-nonenal (4-HNE) or malondialdehyde (MDA) are confirmed as markers of oxidative stress-induced lipid peroxidation [ 17 , 18 ].

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