Embryonic and Induced Pluripotent Stem Cells and Their Differentiation in the Cardiomyocyte Direction in the Presence of Dimethyl Sulfoxide

Budash, Galyna; Bilko, Nadiia

doi:10.3103/s0095452719010055

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…This improvement was determined to be even higher than that observed in known antioxidants, such as N-acetylcysteine [ 8 ] Similarly, in colon cancer cells, low DMSO doses (0.1–1.5%) were shown to reduce the cellular levels of reactive oxygen species (ROS) and the cells proliferative activity relative to the control [ 9 ]. Literature has further shown that the addition of DMSO (1%) to culture medium at the formation stage of cardiomyocyte progenitor cells stimulates the differentiation of pluripotent stem cells (PSCs) into cardiomyocytes by a 1.5-fold increase [ 10 ]. Conversely, the addition of DMSO before the initiation of embryoid bodies is reported to suppress the differentiation of PSCs and therefore, the formation of cardiomyocytes [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Literature has further shown that the addition of DMSO (1%) to culture medium at the formation stage of cardiomyocyte progenitor cells stimulates the differentiation of pluripotent stem cells (PSCs) into cardiomyocytes by a 1.5-fold increase [ 10 ]. Conversely, the addition of DMSO before the initiation of embryoid bodies is reported to suppress the differentiation of PSCs and therefore, the formation of cardiomyocytes [ 10 ]. Engineered cardiac tissue, which is generated from differentiated PSCs, are reported to have enhanced engraftment rates, as well as increased survival and progressive maturation of human cardiomyocytes [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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The Implication of Low Dose Dimethyl Sulfoxide on Mitochondrial Function and Oxidative Damage in Cultured Cardiac and Cancer Cells

et al. 2021

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Although numerous studies have demonstrated the biological and multifaceted nature of dimethyl sulfoxide (DMSO) across different in vitro models, the direct effect of “non-toxic” low DMSO doses on cardiac and cancer cells has not been clearly explored. In the present study, H9c2 cardiomyoblasts and MCF-7 breast cancer cells were treated with varying concentrations of DMSO (0.001–3.7%) for 6 days. Here, DMSO doses < 0.5% enhanced the cardiomyoblasts respiratory control ratio and cellular viability relative to the control cells. However, 3.7% DMSO exposure enhanced the rate of apoptosis, which was driven by mitochondrial dysfunction and oxidative stress in the cardiomyoblasts. Additionally, in the cancer cells, DMSO (≥0.009) led to a reduction in the cell’s maximal respiratory capacity and ATP-linked respiration and turnover. As a result, the reduced bioenergetics accelerated ROS production whilst increasing early and late apoptosis in these cells. Surprisingly, 0.001% DMSO exposure led to a significant increase in the cancer cells proliferative activity. The latter, therefore, suggests that the use of DMSO, as a solvent or therapeutic compound, should be applied with caution in the cancer cells. Paradoxically, in the cardiomyoblasts, the application of DMSO (≤0.5%) demonstrated no cytotoxic or overt therapeutic benefits.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Implication of Low Dose Dimethyl Sulfoxide on Mitochondrial Function and Oxidative Damage in Cultured Cardiac and Cancer Cells

et al. 2021

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Cultivation and cryopreservation of rat stem cells and their interaction with lyophilised acellular matrix

Fedoniuk,

Dovgalyuk,

Furka

et al. 2023

bmbr

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With the rapid development of regenerative medicine in the 21st century, the study of the therapeutic potential of stem cells in both preclinical research and clinical trials has become particularly relevant. Preclinical studies on animals allow for a detailed understanding of the mechanisms of action of allogzeneic cell preparations, exploring their regenerative activity, pharmacodynamics, and potential side effects. The purpose of the study was to select optimal conditions for obtaining, cultivating, and cryopreserving mesenchymal stem cells from rats and analyse their interaction with the lyophilised acellular matrix. The enzymatic method was applied to obtain primary cell cultures from the umbilical cord, dermis, and muscles of Rattus norvegicus fetuses. Cell cultures were cultivated in vitro, and cell line proliferation rates were analysed using an inverted microscope. In addition, cryopreservation was performed to store cellular materials. The interaction of mesenchymal stem cells with an acellular matrix and cryopreservation of the obtained cells was at the 4 and 5th passages. It was shown that the optimal nutrient medium for cultivating the obtained lines of mesenchymal stem cells from the umbilical cord and dermis of rat fetuses is DMEM/F12 Advanced. It was established that the method of thawing the cell suspension by 10-fold dilution of dimethyl sulfoxide is more effective than the alternative method of immediate removal of cryoprotectant by centrifugation. The lyophilised acellular dermal matrix was found to have a cytotoxic effect on all cultured rat cells, while the pericardial matrix showed a positive effect on the growth of the investigated cell lines. Thus, the optimal nutrient medium and conditions for freezing/thawing of rat stem cells were selected, and the effect of lyophilised acellular matrix, planned for therapeutic use, on the obtained cell lines was determined

show abstract

Embryonic and Induced Pluripotent Stem Cells and Their Differentiation in the Cardiomyocyte Direction in the Presence of Dimethyl Sulfoxide

Cited by 2 publications

References 19 publications

The Implication of Low Dose Dimethyl Sulfoxide on Mitochondrial Function and Oxidative Damage in Cultured Cardiac and Cancer Cells

The Implication of Low Dose Dimethyl Sulfoxide on Mitochondrial Function and Oxidative Damage in Cultured Cardiac and Cancer Cells

Cultivation and cryopreservation of rat stem cells and their interaction with lyophilised acellular matrix

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