Embryonic development in repeat breeder and virgin heifers seven days after insemination

Linares, T.

doi:10.1016/0378-4320(82)90002-1

Cited by 34 publications

(14 citation statements)

References 16 publications

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“…Ultrastructural investigations of the bovine embryo at different stages of morphological development have previously been published [Brackett et al, 1980;Linares, 1981;Massip et al, 1981]. However, only limited ultrastructural studies concerning the possible damage caused by freezing and thawing have been undertaken.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructure of Bovine Embryos Frozen and Thawed by a Two-Step Freezing Method

Hyttel¹,

Lehn-Jensen

Greve

1986

Cells Tissues Organs

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An ultrastructural evaluation of a rapid tow-step freezing method, by which 6–7-day-old bovine embryos equilibrated in 1.4 M glycerol in Dulbecco’s phosphate-buffered saline were frozen and thawed, was undertaken. In all non-frozen control embryos trophoblastic and embryonic cells formed a spherical structure enclosed by an intact zona pellucida. The spacial arrangement of the cells of the frozen embryos was less regular and the surrounding zona pellucida was damaged in approximately half of the cases. Some embryonic cells had increased electron density and lysosomal content showing reaction sites for acid phosphatase. In all frozen embryos, cytoplasmic defects appearing as non-membrane-bound ‘empty spaces’ were observed more frequently in the trophoblastic cells than in the embryonic cells. Culture of frozen embryos for 24 h revealed that cells appearing non-defective after culture may have the capability of organizing a viable embryonic structure. It was found that the most commonly used freezing method is associated with certain morphological changes. However, no additional cryoinjuries were observed in comparisons with the more complicated freezing procedures using dimethylsulfoxide as cryoprotectant.

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Section: Introductionmentioning

confidence: 99%

Ultrastructure of Bovine Embryos Frozen and Thawed by a Two-Step Freezing Method

Hyttel¹,

Lehn-Jensen

Greve

1986

Cells Tissues Organs

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“…Intrinsic embryo factors are also related with this syndrome. Linares (1982) observed that blastocysts collected in RBCs had more abnormalities than those of control cows (3:1), and the oocyte collection rate was also lower, possibly due to a degenerative process initiated after fertilization. It was determined that the reduction of embryo viability could be associated with the reduction of nutrients exchange capacity and other regulatory substances.…”

Section: Wwwintechopencommentioning

confidence: 90%

Clinical Approach to the Repeat Breeder Cow Syndrome

Marı́n¹,

Moreno²,

Calero³

2012

A Bird's-Eye View of Veterinary Medicine

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“…However, while some studies have shown a positive association between progesterone and embryo survival rate in cattle (Ahmad et al. 1996), others have not recorded any such association (Ayalon 1978; Bulman and Lamming 1978; Linares 1981).…”

Section: Introductionmentioning

confidence: 99%

Effect of Complex Vertebral Malformation on Luteal Function in Holstein Cows During Oestrous Cycle and Early Pregnancy

Ghanem

Suzuki

Kasuga

et al. 2009

Reproduction in Domestic Animals

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The reason why cows carrying the mutation of complex vertebral malformation (CVM) show poor reproductive capability although they carry only one mutant allele is still not fully understood. Monitoring the progesterone profiles during oestrous cycle and early pregnancy in carrier cows might help explain their lowered reproductive capability. Progesterone concentration was measured in 19 CVM carrier cows and 21 control cows during oestrous cycle and early pregnancy. Milk samples were collected from all cows starting on the day of artificial insemination until day 45 post-AI. Progesterone was measured in skim milk using enzyme-linked immunosorbent assay (ELISA). Progesterone concentration was significantly reduced on day 7 (p < 0.05) and day 9 (p < 0.01) post-insemination in conceived CVM carrier cows when compared with that in control conceived cows. The mean progesterone concentration during early pregnancy was significantly lower (p < 0.05) in conceived cows with CVM than that of control cows in the same period. However, the mean progesterone concentration did not differ significantly (p = 0.072) in CVM cows that showed fertilization failure or embryonic death than that of control cows. Additionally, of 13 conceived control cows, eight cows (61.5%) showed normal luteal function. In contrast, of nine conceived CVM cows, only four cows (44.4%) showed normal luteal function. The conception rate was 47.4% in CVM carrier cows and 61.9% in control cows, but this difference did not reach significance. In conclusion, progesterone concentration might be lowered during early pregnancy in conceived CVM cows compared with that in control cows.

show abstract

Embryonic development in repeat breeder and virgin heifers seven days after insemination

Cited by 34 publications

References 16 publications

Ultrastructure of Bovine Embryos Frozen and Thawed by a Two-Step Freezing Method

Ultrastructure of Bovine Embryos Frozen and Thawed by a Two-Step Freezing Method

Clinical Approach to the Repeat Breeder Cow Syndrome

Effect of Complex Vertebral Malformation on Luteal Function in Holstein Cows During Oestrous Cycle and Early Pregnancy

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