Embryonic Development of Cuban Gar (<i>Atractosteus tristoechus</i>) Under Laboratory Conditions

Comabella, Yamilé; Canabal, Javier; Hurtado, Andrés; García-Galano, Tsai

doi:10.1111/ahe.12101

Cited by 5 publications

(4 citation statements)

References 25 publications

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Section: Discussionmentioning

confidence: 99%

“…Subsequent manipulation with zygotes and raising the offspring is not complicated and the similarity of gar and sturgeon eggs should make injecting relatively straightforward. One potentially confounding factor worth mentioning is that gar embryos, like those of lamprey, lack maternal pigmentation, making it difficult to discern when cleavage begins (Comabella et al, 2014). While other non-teleost ray-finned fish species are likely well suited to CRISPR/Cas9 F0 mutagenesis, two key groups of earlydiverging vertebrates, the chondrichthyans, and hagfish, are unlikely to be amenable to this method, at least as described here.…”

Section: Sterlet Is Highly Amenable To Crispr/cas9 F0 Mutagenesismentioning

confidence: 99%

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Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates

Štundl

Soukup

Franěk

et al. 2022

Front. Cell Dev. Biol.

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In the last decade, the CRISPR/Cas9 bacterial virus defense system has been adapted as a user-friendly, efficient, and precise method for targeted mutagenesis in eukaryotes. Though CRISPR/Cas9 has proven effective in a diverse range of organisms, it is still most often used to create mutant lines in lab-reared genetic model systems. However, one major advantage of CRISPR/Cas9 mutagenesis over previous gene targeting approaches is that its high efficiency allows the immediate generation of near-null mosaic mutants. This feature could potentially allow genotype to be linked to phenotype in organisms with life histories that preclude the establishment of purebred genetic lines; a group that includes the vast majority of vertebrate species. Of particular interest to scholars of early vertebrate evolution are several long-lived and slow-maturing fishes that diverged from two dominant modern lineages, teleosts and tetrapods, in the Ordovician, or before. These early-diverging or “basal” vertebrates include the jawless cyclostomes, cartilaginous fishes, and various non-teleost ray-finned fishes. In addition to occupying critical phylogenetic positions, these groups possess combinations of derived and ancestral features not seen in conventional model vertebrates, and thus provide an opportunity for understanding the genetic bases of such traits. Here we report successful use of CRISPR/Cas9 mutagenesis in one such non-teleost fish, sterlet Acipenser ruthenus, a small species of sturgeon. We introduced mutations into the genes Tyrosinase, which is needed for melanin production, and Sonic hedgehog, a pleiotropic developmental regulator with diverse roles in early embryonic patterning and organogenesis. We observed disruption of both loci and the production of consistent phenotypes, including both near-null mutants’ various hypomorphs. Based on these results, and previous work in lamprey and amphibians, we discuss how CRISPR/Cas9 F0 mutagenesis may be successfully adapted to other long-lived, slow-maturing aquatic vertebrates and identify the ease of obtaining and injecting eggs and/or zygotes as the main challenges.

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Section: Discussionmentioning

confidence: 99%

Section: Sterlet Is Highly Amenable To Crispr/cas9 F0 Mutagenesismentioning

confidence: 99%

Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates

Štundl

Soukup

Franěk

et al. 2022

Front. Cell Dev. Biol.

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“…With the onset of yolk sac depletion during development, internal morphological changes occurred to determine the physiological and behavioral capabilities. These changes were successful in optimizing the efficiency of prey capture and increase escape from predators of early life fish survival (Comabella et al, 2013). The present work studied the embryonic developmental stages of Siganus rivulatus from fertilized eggs to yolk sac larval stage after only high dose of hormonal injection at a dose of 100 μg kg -1 LHRHa as a baseline for successful development.…”

Section: Introductionmentioning

confidence: 99%

Embryonic development of the rabbit fish Siganus rivulatus and allometric growth patterns during yolk sac larvae stage produced by hormone-induced broodstock

Fahmy¹,

Wahbi²

2020

Egypt. J. of Aquatic Biolo. and Fish.

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The embryonic development of the rabbitfish Siganus rivulatus produced from induced spawning broodstock is described under controlled laboratory conditions. The present work studied the embryonic development of the laboratory reared Siganus rivulatus from eggs to yolk sac larval stage after high dose of hormonal induction with 100 μg kg-1 of leuteinizing hormone releasing hormone (LHRHa) injection as the best dose for egg quality (baseline). This research was conducted to study the differences in the growth pattern during the yolk sac larval stage between two experiments 100, 50 μg kg-1 as high and low dose of LHRHa injection that whereby recognize the appropriate dose for seed quality produced. Embryonic stages of Siganus rivulatus were observed. The morphometric parameters which were measured in larvae included: total length (TL), head length (HL), trunk length (TrL), head trunk length [pre-anal length (PreAl)] and tail length (TaL). Also, the Post-anal to Pre-anal ratio was calculated for each experiment. The allometric growth patterns were calculated by a power function of total length (TL) and describing the relation between every character (Y) by the growth coefficient (b), (Y = aTL b). The seven embryonic developmental periods (stages) which were studied included: fertilized eggs, cleavage, morula, blastula, gastrula, segmentation and hatching stages. The mean total length of the newly hatched larvae was 1.975 mm and increased linearly until the end of the experiments with a high correlation and regression coefficient in both injected groups. Before the onset of exogenous feeding, there was a clear growth priority in the head region in relation to total length in larvae of the high dose treated group. Tail region had strong positive allometric growth in larvae of both injected groups; with faster larval growth rate in those of the high dose injected group. In conclusion the differences in the growth rate were directly proportional to the dose of injection.

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“…These concepts directly contribute to estimating fish biomass from length data (Kapiris & Klaoudatos, 2011;Konan, 2017;Randall, 2002;Rodríguez et al, 2017;Sangun et al, 2007), providing a species condition factor (Jisr et al, 2018;Prestes et al, 2019), helping to predict fish feed consumption (Wiff & Roa-Ureta, 2008) and estimate stocking densities under land-base farm-like conditions (Merino et al, 2022). However, allometry also differentiates life-history and morphologic traits, such as growth patterns during early developmental stages (Comabella et al, 2013;Lackmann et al, 2022;Mitra et al, 2016;Osse & Boogaart, 1995;Stoltz et al, 2005) and fish behaviour (Baldauf et al, 2010;Barros et al, 2015;Dunlap et al, 2019;Taugbol et al, 2020), which in turn influence population and community structure and set distinctive spatial, evolutionary and geographical distributions (Davenport, 2003;Furness et al, 2021;Garita-Alvarado & Ornelas-Garcia, 2021;Kapiris & Klaoudatos, 2011;Oliveira et al, 2018;Summers & Ord, 2022;Vega-Trejo et al, 2022).…”

mentioning

confidence: 99%

Morphological traits for allometric scaling of the European Sea Bass Dicentrarchus labrax (Linnaeus, 1758) from Southern Portugal population

Ana

Navarro

Cavalheri

et al. 2023

Journal of Fish Biology

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The present study aimed to determine the allometric scaling among a selection of morphological traits in European sea bass (Dicentrarchus labrax) to estimate fish body weight. A set of morphological traits (fish body weight, length, height, and width) were directly measured in 146 fish of a recirculating aquaculture system, with body weights ranging from 17.11 to 652.21 g. In addition, a collection of digital imagery of each anesthetized fish from the side and top views were used to estimate other traits (indirect measures). Multiple regression analysis and regression coefficients were calculated using all possible combinations of biometric data (predictors) to estimate fish body weight, applying different numerical fitting models (linear, log-linear, quadratic, exponential). The results showed that the best combination of traits for estimating fish body weight were fish body width, length and height, collected from direct measure (R 2 = 0.995), for a log-linear model fitting, which revealed more accurate determinations than the most commonly used length-weight relationship. Nevertheless, other combinations of morphological traits and fitting models were also found to be suitable in successfully predict fish body weight, with variability ranging between 92.5% and 98.5%. For indirect measures, the best predictor was a combination of traits from top view (width, eye distance and area without fins) fitted with a log-linear function. These results comprise a relevant baseline in supporting the high potential of noninvasive methods to accurately follow the growth of European sea bass juveniles, recurring to imagery analysis of anesthetized fish. It has major potential applications in feeding consumption trials and fish growth models, as it allows for continuously following up fish growth under different experimental conditions without therein distress derived from manipulation.

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Embryonic Development of Cuban Gar (Atractosteus tristoechus) Under Laboratory Conditions

Cited by 5 publications

References 25 publications

Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates

Efficient CRISPR Mutagenesis in Sturgeon Demonstrates Its Utility in Large, Slow-Maturing Vertebrates

Embryonic development of the rabbit fish Siganus rivulatus and allometric growth patterns during yolk sac larvae stage produced by hormone-induced broodstock

Morphological traits for allometric scaling of the European Sea Bass Dicentrarchus labrax (Linnaeus, 1758) from Southern Portugal population

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