Embryonic exposure to carbendazim induces the transcription of genes related to apoptosis, immunotoxicity and endocrine disruption in zebrafish (Danio rerio)

Jiang, Jinhua; Wu, Shenggan; Wu, Changxing; An, Xingwei; Cai, Leiming; Zhao, Xueping

doi:10.1016/j.fsi.2014.09.037

Cited by 59 publications

(21 citation statements)

References 49 publications

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“…The innate immune system plays critical protective and defensive roles during fetal and early life stages Several investigations have highlighted the significant effects of environmental and endocrine‐disrupting chemicals on the transcription levels of chemokines and cytokines and immune system activity . Here, we further demonstrated that the mRNA levels of innate immune‐related genes, CXCL‐1, IL‐1β, and IL‐8, are markedly reduced in fetuses obtained from 5 mg/kg/d ENN B1‐injected female mice (Figure A).…”

Section: Discussionsupporting

confidence: 57%

Enniatin B1 exerts embryotoxic effects on mouse blastocysts and induces oxidative stress and immunotoxicity during embryo development

Huang

Wang

Chan

2018

Environmental Toxicology

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Section: Discussionsupporting

confidence: 57%

Enniatin B1 exerts embryotoxic effects on mouse blastocysts and induces oxidative stress and immunotoxicity during embryo development

Huang

Wang

Chan

2018

Environmental Toxicology

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“…Combined with downregulation of the vtg1 gene when exposued to R ‐metalaxyl, it was possible that R ‐metalaxyl could disturb the synthesis of vtg , which would further affect the transportation of nutrients followed by growth and development inhibition of zebrafish embryos. Cytochrome P450 aromatase ( cyp ) plays the leading role in the biosynthesis of steroid hormones, and cyp19 plays a crucial role in the step of transforming androgens into estrogens . Our study showed that cyp19a decreased significantly after exposure to 100 mg · L ‐1 R ‐metalaxyl, and cyp19b was downregulated after being treated with 200 mg · L ‐1 R ‐metalaxyl, which possibly means that the synthesis of sex hormones was affected.…”

Section: Resultsmentioning

confidence: 71%

“…Cytochrome P450 aromatase (cyp) plays the leading role in the biosynthesis of steroid hormones, and cyp19 plays a crucial role in the step of transforming androgens into estrogens. 29,30 Our study showed that cyp19a decreased significantly after exposure to 100 mg · L -1 Rmetalaxyl, and cyp19b was downregulated after being treated with 200 mg · L -1 R-metalaxyl, which possibly means that the synthesis of sex hormones was affected.…”

Section: Developmental Toxicity Of Rac-metalaxyl and R-metalaxylmentioning

confidence: 72%

Enantioselectivity in Developmental Toxicity of rac‐metalaxyl and R‐metalaxyl in Zebrafish (Danio rerio) Embryo

Zhang

Chen

et al. 2016

Chirality

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Enantioselectivity of chiral pesticides in environmental safety has attracted more and more attention. In this study, we evaluated the enantioselective toxicity of rac-metalaxyl and R-metalaxyl to zebrafish (Danio rerio) embryos through various malformations including pericardial edema, yolk sac edema, crooked body, and short tails. The results showed that there were significant differences in toxicity to zebrafish embryos caused by rac-metalaxyl and R-metalaxyl, and the LC50 s at 96 h are 416.41 (353.91, 499.29) mg · L(-1) and 320.650 (279.80, 363.46) mg · L(-1) , respectively. In order to explore the possible mechanism of the development defects, the genes involved in the hypothalamic-pituitary-gonadal axis (vtg1, vtg2, cyp17, cyp19a, cyp19b) and hypothalamic-pituitary-thyroid axis (dio1, dio2, nis, tg, tpo) were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). The results revealed that there were no significant differences in the expression of vtg1, vtg2, cyp17, cyp19a, and cyp19b after exposure to rac-metalaxyl. However, the expression of vtg1, cyp19a, and cyp19b decreased significantly after exposure to R-metalaxyl. And likewise, rac-metalaxyl only caused the upregulation of dio2, while R-metalaxyl suppressed the expression of dio1 and tpo and induced the expression of dio2 and nis. The change of gene expression may cause the enantioselectivity in developmental toxicity in zebrafish embryo. The data provided here will be helpful for us to comprehensively understand the potential ecological risks of the currently used chiral fungicides. Chirality 28:489-494, 2016. © 2016 Wiley Periodicals, Inc.

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“…Apoptosis is characterized by chromatin condensation, DNA fragmentation, plasma membrane blebbing (i.e., externalization of phosphatidylserine), and cell shrinkage due to reduction in cytoplasm and organelles (Kerr, ; Majno and Joris, ; Rich et al, ). Numerous studies utilized zebrafish embryo models to test a number of chemicals for apoptosis (Jiang et al, ; Zeng et al, ; Wu et al, ; Zhu et al, ). It was unknown whether the two indoxacarb enantiomers induced apoptosis during zebrafish development.…”

Section: Introductionmentioning

confidence: 99%

Toxic effects of indoxacarb enantiomers on the embryonic development and induction of apoptosis in zebrafish larvae (Danio rerio)

Fan

Feng

Lai

et al. 2015

Environmental Toxicology

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Indoxacarb is a highly potent insecticide widely used to control Lepidoptera insects in vegetable, tea, cotton, and rice fields. It can run off into aquatic environments. It is consisted of two enantiomers. Environmental risks and aquatic toxicity of indoxacarb enantiomers have not been fully investigated. In this study, zebrafish (Danio rerio) embryos were exposed to varying concentrations of (-)-R-indoxacarb and (+)-S-indoxacarb until 96-h post-fertilization (hpf) to assess the embryonic toxicity. (-)-R-indoxacarb was 1.3-fold more toxic than (+)-S-isomer to zebrafish embryos at 96 hpf. (-)-R-indoxacarb exhibited reduction in body length and pericardial edema compared with (+)-S-indoxacarb. (-)-R-indoxacarb decreased the hatching rate sixfold greater than (+)-S-indoxacarb. The rate of pericardial edema induced by (-)-R-indoxacarb was 2.5 times greater than that by (+)-S-indoxacarb. The heart rate of the larvae exposed to (-)-R-indoxacarb was 30% lower than that to (+)-S-indoxacarb. In addition, exposure to the chiral isomers resulted in significant increases in apoptosis; interestingly (-)-R-indoxacarb induced apoptosis in the heart area, whereas (+)-S-indoxacarb induced apoptosis in the head area. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 7-16, 2017.

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Embryonic exposure to carbendazim induces the transcription of genes related to apoptosis, immunotoxicity and endocrine disruption in zebrafish (Danio rerio)

Cited by 59 publications

References 49 publications

Enniatin B1 exerts embryotoxic effects on mouse blastocysts and induces oxidative stress and immunotoxicity during embryo development

Enniatin B1 exerts embryotoxic effects on mouse blastocysts and induces oxidative stress and immunotoxicity during embryo development

Enantioselectivity in Developmental Toxicity of rac‐metalaxyl and R‐metalaxyl in Zebrafish (Danio rerio) Embryo

Toxic effects of indoxacarb enantiomers on the embryonic development and induction of apoptosis in zebrafish larvae (Danio rerio)

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