Embryonic stem cell-like subpopulations are present within Schwannoma

Kilmister, Ethan J.; Patel, Josie; Bockett, Nicholas; Chang-McDonald, Bridget; Sim, Dalice; Wickremesekera, Agadha; Davis, Paul F.; Tan, Swee T.

doi:10.1016/j.jocn.2020.09.037

Cited by 7 publications

(7 citation statements)

References 56 publications

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“…So far, there have been few other studies of multiple CSC markers in the VS, making our study one of the rst detections of CSC in the VS. Kilmister et al were able to detect a group of cells expressing Oct4, SOX2, Nanog, Klf4, and c-myc in a much smaller population of VS. They also inferred a subpopulation of CSC in the VS, but did not associate it with tumor growth [10].…”

Section: Discussionmentioning

confidence: 98%

“…In tumors of the central nervous system, cells expressing CSC markers could be detected in glioblastoma, highlighting their importance [24,25]. In benign tumors, there has been limited investigation whether CSC could have an impact on size progression, for example in meningioma [10,24].…”

Section: Discussionmentioning

confidence: 99%

“…In malignant tumors, such as bladder, breast and colon cancer, the in uence of CSC on progression has already been demonstrated [8,9]. In benign tumors, such as VS, there are only few studies concerning this matter [10]. In the presented study we investigated the expression of the CSC markers c-myc, C-X-C motif chemokine receptor 4 (CXCR4), SRY-Box transcription factor 2 (SOX2), Nestin, Nanog, Octamer-binding protein 4 (Oct4), Kruppel like factor 4 (Klf4), CD44, CD133, Sal-like protein 4 (Sall4) and CD45, which are important for the maintenance of self-differentiation and pluripotency in CSC.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Impact of cancer stem cell marker expression in vestibular schwannoma progression

Klause

Schildhauer

Strauß

et al. 2023

Preprint

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Background: Vestibular schwannoma (VS), a benign tumor originating from the Schwann cells surrounding the 8th cranial nerve, is one of the most common intracranial tumors. Affected patients suffer from symptoms such as impaired hearing, dizziness and dysfunction of surrounding cranial nerves, which increase with the size of the VS. Although various factors influencing VS growth have been investigated, the molecular causes remain unclear. Cancer stem cells (CSC) are already known from malignant tumor entities to have the ability to self-renew and differentiate to generate and drive tumor growth. Therefore, our study investigated the influence of the expression of cancer stem cell markers in the VS progression. Methods: An exploratory study of eleven CSC markers was performed in 165 VS of different tumor volume by quantitative real-time polymerase chain reaction and correlated with preoperative tumor volume. In addition, we investigated the CSC markers Nestin, Nanog, CD44 and CD45 in selected VS primary cultures by immunofluorescence and flow cytometry. Finally, a copy number variation analysis of 26 tumor samples was performed to investigate gain or loss of CSC markers at DNA level. Results: Our results showed a strong correlation among CSC marker expression and a correlation of the tumor volume with eight CSC markers. In the VS, in particular, the expression of the markers CXCR4, CD44, CD45, Nestin, and CD133 were related to volume increase, whereas expression of SOX2, c-myc and Klf4 showed a negative correlation with the tumor volume. Using flow cytometry and immunofluorescence analysis, population of cells were detected expressing CD44 and CD45 as well as Nestin and Nanog simultaneously. Conclusion: This suggests that there is a not previously described subpopulation of CSC in VS, which is associated with an increasing tumor volume. Due to the limited treatment options available for VS, these findings could offer the possibility of targeting CSC pharmacologically to prevent tumor progression in the VS. Trial registration: The study was approved by the ethics committee of the Martin-Luther-University Halle-Wittenberg (approval number 2020-122) and ensures written informed consent of all patients.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of cancer stem cell marker expression in vestibular schwannoma progression

Klause

Schildhauer

Strauß

et al. 2023

Preprint

View full text Add to dashboard Cite

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“…In fact, OCT4, SOX2, KLF4, L-MYC, and LIN28 are all known as oncogenes or proto-oncogenes ( 87 - 89 ). In particular, strong expression of OCT4 and SOX2 was confirmed in Schwannoma ( 90 ), and LIN28 also contributes to embryonal tumor progression in the CNS ( 91 ). Moreover, inhibition of p53, a potent tumor suppressor, further amplifies concerns about tumor development ( 92 ).…”

Section: Direct Conversion As An Alternative To Differentiationmentioning

confidence: 99%

Direct Conversion to Achieve Glial Cell Fates: Oligodendrocytes and Schwann Cells

Yun

Kim

Lee

2022

IJSC

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Glia have been known for its pivotal roles in physiological and pathological conditions in the nervous system. To study glial biology, multiple approaches have been applied to utilize glial cells for research, including stem cell-based technologies. Human glial cells differentiated from pluripotent stem cells are now available, allowing us to study the structural and functional roles of glia in the nervous system, although the efficiency is still low. Direct conversion is an advanced strategy governing fate conversion of diverse cell types directly into the desired lineage. This novel strategy stands as a promising approach for preliminary research and regenerative medicine. Direct conversion employs genetic and environmental cues to change cell fate to that with the required functional cell properties while retaining maturity-related molecular features. As an alternative method, it is now possible to obtain a variety of mature cell populations that could not be obtained using conventional differentiation methods. This review summarizes current achievements in obtaining glia, particularly oligodendrocytes and Schwann cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Impact of cancer stem cell marker expression in vestibular schwannoma progression

Klause

Schildhauer

Strauß

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: Vestibular schwannoma (VS), a benign tumor originating from the Schwann cells surrounding the 8th cranial nerve, is one of the most common intracranial tumors. Affected patients suffer from symptoms such as impaired hearing, dizziness and dysfunction of surrounding cranial nerves, which increase with the size of the VS. Although various factors influencing VS growth have been investigated, the molecular causes remain unclear. Cancer stem cells (CSC) are already known from malignant tumor entities to have the ability to self-renew and differentiate to generate and drive tumor growth. Therefore, our study investigated the influence of the expression of cancer stem cell markers in the VS progression. Methods: An exploratory study of eleven CSC markers was performed in 165 VS of different tumor volume by quantitative real-time polymerase chain reaction and correlated with preoperative tumor volume. In addition, we investigated the CSC markers Nestin, Nanog, CD44 and CD45 in selected VS primary cultures by immunofluorescence and flow cytometry. Finally, a copy number variation analysis of 26 tumor samples was performed to investigate gain or loss of CSC markers at DNA level. Results: Our results showed a strong correlation among CSC marker expression and a correlation of the tumor volume with eight CSC markers. In the VS, in particular, the expression of the markers CXCR-4 (r=0.23, p=0.0045), CD44 (r=0.24, p=0.0041), CD45 (r=0.17, p=0.046), Nestin (r=0.21, p=0.014), and CD133 (r=0.23, p=0.0057) were related to volume increase, whereas expression of SOX2 (r=-0.24, p=0.0031), c-myc (r=-0.19, p=0.020) and Klf4 (r=-0.22, p=0.0088) showed a negative correlation with the tumor volume. Using flow cytometry and immunofluorescence analysis, population of cells were detected expressing CD44 and CD45 as well as Nestin and Nanog simultaneously. Conclusion: This suggests that there is a not previously described subpopulation of CSC in VS, which is associated with an increasing tumor volume. Due to the limited treatment options available for VS, these findings could offer the possibility of targeting CSC pharmacologically to prevent tumor progression in the VS.

show abstract

Embryonic stem cell-like subpopulations are present within Schwannoma

Cited by 7 publications

References 56 publications

Impact of cancer stem cell marker expression in vestibular schwannoma progression

Impact of cancer stem cell marker expression in vestibular schwannoma progression

Direct Conversion to Achieve Glial Cell Fates: Oligodendrocytes and Schwann Cells

Impact of cancer stem cell marker expression in vestibular schwannoma progression

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