Emerge of bla NDM-1 and bla OXA-48-like harboring carbapenem-resistant Klebsiella pneumoniae isolates from hospitalized patients in southwestern Iran

Hosseinzadeh, Zahra; Ebrahim‐Saraie, Hadi Sedigh; Sarvari, Jamal; Mardaneh, Jalal; Dehghani, Behzad; Rokni-Hosseini, Seyed Mohammad Hossein; Motamedifar, Mohammad

doi:10.1016/j.jcma.2017.08.015

Cited by 54 publications

(45 citation statements)

References 23 publications

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“…The highest antibiotic resistance was observed for cephalosporins and carbapenems while the least resistance to colistin and trimethoprim/ sulfamethoxazole. In the present study, the rate of antibiotic resistance with some variation which may arise from the source of infections and geographical distribution was comparable with the previous reported studies in Iran (18,20,26,27). The administration of multiple antibiotics has been reported as a risk factor for carbapenem resistance acquisition (28).…”

Section: Discussionsupporting

confidence: 90%

“…The first report of OXA-48 gene in Iran was by Azimi et al in Tehran 2014, in 27 carbapenem-resistant K. pneumoniae isolates recovered from burn patients (1). It was subsequently re-identified in Iran (20,27). The widespread prevalence of OXA-48-positive carbapenem resistant K. pneumoniae strains has been reported in several Asian and European countries including Turkey, Saudi Arabia, Taiwan, China, Russia and France which has become an expanding problem (11,32,(38)(39)(40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City

Jalalvand¹,

Shayanfar²,

Shahcheraghi³

et al. 2020

Iran J Pathol

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City

Jalalvand¹,

Shayanfar²,

Shahcheraghi³

et al. 2020

Iran J Pathol

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“…Using PCR, 40 (74%) of these isolates were found to contain a carbapenemaseencoding gene. These results of CIM are consistent with other findings stated by some other researches [14,[15][16][17][18][19]. With respect to our research, CIM for the first time in Iran was found to be very efficient and low-cost in the recognition of carbapenemaseproducing K. pneumoniae isolates.…”

Section: Resultssupporting

confidence: 92%

“…CIM. Phenotype OXA-48-positive in KPC strains has been stated in several regions, including France, Russia, Turkey, Saudi Arabia, Taiwan, and China, and can be a serious concern in hospital settings [17]. In our study, a small number of carbapenem-resistant isolates lacked the targeted genes.…”

Section: Type Of Samples Sample Distributionmentioning

confidence: 58%

“…Uni-KPC-F (5'-ATGTCACTGTATCGCCGTCT-3') and Uni-KPC-R (5'-TTACTGCCCGTTGACGCCC-3') genes as previously described (16). PCR amplification and primers for the detection of blaCTX-M, blaTEM, blaSHV, blaKPC, blaOXA-48-like, blaNDM, blaVIM, and blaIMP genes was carried out as previously described by Hosseinzadeh et al and Fursova et al [17,18,33,34]. The sequences were aligned and compared using the online BLAST software, (BLAST http: //www.ncbi.nlm.…”

Section: Polymerase Chain Reaction Amplification (Pcr) and Dna Sequenmentioning

confidence: 99%

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High prevalence of extended-spectrum β-lactamase (blaCTX-M-15) and New Delhi metallo-β-lactamase-1 (NDM-1) genes among high-level carbapenem resistance Klebsiella pneumonia: an alarm for our health system

Yaghoubi¹,

Baseri²,

Rasti³

et al. 2018

Af J Clin Exp Micro

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Background: The extended-spectrum β-lactamase (ESBL) carbapenems-resistant Klebsiella isolates are considered one of the most significant challenging in the treatment of patients in hospitals. The aim of this study was to determine the prevalence of important carbapenem resistance genes ESBL subtypes and between K. pneumoniae from patients at hospital in Tehran, Iran. Methods: Fifty-four isolates of K. pneumoniae were isolated from Shariatee Hospital in Tehran from February 2013 to July 2016. Antibiotic testing was done by using the standard disk diffusion method and E-test MIC. The confirmation of carbapenemase activity was performed using an MHT and a new method called the carbapenem inactivation method test (CIM). Finally, a polymerase chain reaction (PCR) and sequencing of related genes was performed. Results: Our PCR data demonstrate that blaCTX-M group's 40 (81.4%) genes were the most prevalent in our hospital followed by group genes blaCTX-M-3 (18.51%) and blaCTX-M-2 (20.38%). The distribution of the CTX-M group revealed that blaCTX-M-15 23 (42.6%) was the dominant subtype. The coexistence of multiple genes included blaTEM, CTX-M and blaSHV, and CTX-M The presence of blaNDM1, blaOXA-48, and blaKPC were identified in the carbapenem-resistant isolates, 22 (40.7%), 10 (18.5%), and 7 (12.9%) respectively. Conclusion: Our research showed that a CIM test for the first time in Iran is possible and has a high facility for the fast identification of carbapenem-resistant Klebsiella (CRK). We are encountered with the emergence of CTX-M, OXA-48, KPC, and NDM1 harboring CRK strains in our hospitals. Therefore, the treatment of patients infected with these isolates will be an important future concern in our clinical settings.

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Investigation of plasmid‐mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran

Jomehzadeh¹,

Ahmadi²,

Bahmanshiri³

2022

Clinical Laboratory Analysis

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Background Extensive and inappropriate use of quinolones has led to growing resistance rates to these broad‐spectrum antibiotics. The present study purposed to investigate the prevalence of plasmid‐mediated quinolone resistance (PMQR) genes in Klebsiella pneumoniae clinical isolates. Method Ninety‐two non‐repetitive K. pneumoniae clinical isolates were confirmed by standard microbiological methods. Antibacterial susceptibility of isolates toward seven agents from the quinolone family was evaluated by the disc diffusion method. Ciprofloxacin minimum inhibitory concentrations (MICs) were determined using the standard agar dilution method. PCR amplification was used to detect the existence of PMQR genes in the studied isolates. Results In the present study, significant quinolones' resistance (40%) was observed in K. pneumoniae isolates, and most of the strains were resistant to nalidixic acid (94.6%) and ofloxacin (45.6%). MIC analysis showed 15 strains were resistant to 6–128 μg/ml of ciprofloxacin, and five were intermediately‐resistant. PMQR genes were detected in 88% of all isolates. Acc(6’)‐Ib‐cr was constituted half of the total PMQR genes detected among ciprofloxacin non‐susceptible isolates. Of 20 ciprofloxacin non‐susceptible isolates, 65% (n = 13) harbored multiple PMQR determinants, and 15 strains were determined as integron carriage. Conclusion The findings of this study indicated considerable resistance against quinolones, which could be correlated with the extensive and inappropriate use of this class of antibiotics as empirical treatment.

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Emerge of bla NDM-1 and bla OXA-48-like harboring carbapenem-resistant Klebsiella pneumoniae isolates from hospitalized patients in southwestern Iran

Cited by 54 publications

References 23 publications

Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City

Evaluation of Phenotypic and Genotypic Characteristics of Carbapnemases-producing Enterobacteriaceae and Its Prevalence in a Referral Hospital in Tehran City

High prevalence of extended-spectrum β-lactamase (blaCTX-M-15) and New Delhi metallo-β-lactamase-1 (NDM-1) genes among high-level carbapenem resistance Klebsiella pneumonia: an alarm for our health system

Investigation of plasmid‐mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae in southwest Iran

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