Emergence and Dissemination of Extended‐Spectrum β‐Lactamase–Producing<i>Escherichia coli</i>in the Community: Lessons from the Study of a Remote and Controlled Population

Woerther, Paul‐Louis; Angebault, Cécile; Lescat, Mathilde; Ruppé, Étienne; Skurnik, David; Mniai, Assiya El; Clermont, Olivier; Jacquier, Hervé; Costa, Ambra; Renard, Magaly; Bettinger, Régis Marc; Epelboin, Loïc; Dupont, Claire; Guillemot, Didier; Rousset, François; Arlet, Guillaume; Denamur, Erick; Djossou, Félix; Andremont, Antoine

doi:10.1086/654883

Cited by 60 publications

(43 citation statements)

References 46 publications

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“…This issue, which has already been studied in other settings [6], was however not explored in this work.…”

Section: Discussionmentioning

confidence: 94%

“…E. coli isolates were tested for susceptibility to amoxicillin, tircacillin, amoxicillin + clavulanic acid, cefotaxime, cefoxitin, ceftazidime, cefepime, ertapenem, nalidixic acid, ciprofloxacin, gentamicin, amikacin, fosfomycin and co-trimoxazole (Bio-Rad, Marne-la-Coquette, France) using the disc diffusion method and presence of ESBL was determined using the double disc-diffusion phenotypic method, as recommended by the Antibiogram Committee of the French Society for Microbiology [4]. To confirm the ESBL identification with molecular methods, total DNA was extracted from E. coli strains, as described [5] and blaCTX-M (group 1), blaTEM and blaSHV genes were amplified by PCR using specific primers, as described [6].…”

Section: Microbiological Analysismentioning

confidence: 99%

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Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Djuikoue

Woerther

Toukam

et al. 2016

J Infect Dev Ctries

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Introduction: During the last decade, the prevalence of the intestinal carriage of extended spectrum beta-lactamases -producing Escherichia coli (ESBL-E. coli) has continued to increase worldwide in the community, especially in developing countries. Hence, we undertook a study to determine the ESBL-E. coli fecal carriage rate and the associated risk factors in Cameroonian women. Methodology: A total of 86 women suspected of community-acquired urinary tract infections (UTI) were included in 10 health structures from May 2011 to April 2012. After filling a questionnaire, they provided a stool sample that was plated on selective media for ESBL producing bacteria. The identification of strains was obtained with mass spectrometry and the antibiotic susceptibility by disk diffusion in agar media. The ESBL type was determined by PCR. The relative abundance of ESBL-E. coli was measured for positive samples. Eventually, the presence of antibiotics in stool was assessed. Results: The carriage rate of ESBL-E. coli was 57/86 (66.3%). Phenotypic and molecular characterization showed that all ESBL-E. coli strains contained group 1 CTX-M enzymes. Multivariate analysis showed that ESBL-E. coli fecal carriage was associated with the presence of antibiotics in stools (p < 0.05). Although not significant, mean ESBL relative abundance tended to be higher in patients with antibiotic exposure. Conclusions: Our results show that the carriage of ESBL-E. coli fecal carriage in women with UTI suspicion from the Cameroonian community is extremely high and associated with recent antibiotic intake.

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“…This issue, which has already been studied in other settings [6], was however not explored in this work.…”

Section: Discussionmentioning

confidence: 94%

Section: Microbiological Analysismentioning

confidence: 99%

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Djuikoue

Woerther

Toukam

et al. 2016

J Infect Dev Ctries

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“…Keeping pets at home was accused of causing up to sevenfold increase in the carriage rates (33). There are also reports, in contrast, reporting no significant increase in carriage in those who have contact with animals or who keep pets (3,13). We could not demonstrate a significant association between keeping pets and ESBL carriage rates (P = 0.277).…”

Section: Discussionmentioning

confidence: 99%

“…Considering the probable increase in infections with ESBL-producing bacteria in patients who are already carriers of these bacteria, recent studies on the rates of carriage in the community and the relation between carriage and infections are attracting attention. While rates of 1%-8% carriage in healthy populations were reported in various European countries between 2003 and 2007, the rates were found to be increasing over time (2)(3)(4). On the other hand, up to 50%-60% rates of carriage were reported from countries in Asia and Africa such as Egypt (5) and China (6).…”

Section: Introductionmentioning

confidence: 99%

Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in a Turkish community

et al. 2017

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The aim of this study was to determine the prevalence of fecal carriage of extended-spectrum beta-lactamase (ESBL)producing bacteria, enzyme types, and risk factors affecting colonization. Materials and methods:A total of 576 stool samples from outpatients were examined between October 2012 and May 2013. Screening was done with selective EMB plates. ESBL were detected by double-disk synergy and confirmed agar strip gradient methods. Enzyme types were determined by PCR. Results:The prevalence of fecal carriage was found as 30% (173 of 576). Recent use of antibiotics, hospitalization and surgical operation, diabetes, crowded household populations, and old age were associated with higher carriage rates. Of the ESBL-producing bacteria, 87.5% were positive for bla CTX-M genes. Of the bla CTX-M gene-positive isolates, 95.2% were positive for bla CTX-M-1 genes; among these, 82.2% were positive for bla CTX-M-3 and 67.7% were positive for bla CTX-M-15 genes while 62.5% isolates were positive for both bla CTX-M-3 and bla CTX-M-15 genes Conclusions: A high rate (30%) of fecal carriage of ESBL bacteria was found in an adult population. The predominant beta-lactamase enzyme types were CTX-M-3 and CTX-M-15.

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“…At the same time, it was noted that there was an increase in antibiotic usage, especially the beta-lactams. This variability probably occurred because of the repeated introduction of new strains and plasmids and from inter-individual dissemination (14) In Central and South America ESBL, rates in Klebsiella varied from 30 to 60% in countries such as Brazil, Columbia and Venezuela (15). The ESBL strains included SHV-2, 5, CTX-M and even non-TEM and non-SHV with no geographical predilection.…”

Section: Recent Epidemiological Datamentioning

confidence: 99%

The Changing Epidemiology of Extended Spectrum Beta-Lactamases (ESBL) Infections of the Urinary Tract Focusing on Clinical Resistance and Therapeutic Options

Antony¹

2011

Clinical Management of Complicated Urinary Tract Infection

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Emergence and Dissemination of Extended‐Spectrum β‐Lactamase–ProducingEscherichia coliin the Community: Lessons from the Study of a Remote and Controlled Population

Cited by 60 publications

References 46 publications

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Fecal carriage of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella spp. in a Turkish community

The Changing Epidemiology of Extended Spectrum Beta-Lactamases (ESBL) Infections of the Urinary Tract Focusing on Clinical Resistance and Therapeutic Options

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