Emergence and Rapid Spread of Tetracycline-Resistant <i>Vibrio cholerae</i> Strains, Madagascar

Dromigny, Jacques-Albert; Rakoto-Alson, O; Rajaonatahina, Davidra; Migliani, R.; Ranjalahy, J.; Mauclère, Philippe

doi:10.3201/eid0803.010258

Cited by 27 publications

(13 citation statements)

References 3 publications

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“…Finally, combinatorial treatments of NO and an antibiotic were assessed on V. cholerae biofilms. Tetracycline is the usual antibiotic of choice to treat V. cholerae infections, but resistance to this drug is increasing (Dromigny et al, 2002). Although tetracycline (14 mM) alone, at a concentration below the minimum inhibitory concentration (approximated at 22 mM in this study) for V. cholerae, had very limited effect on biofilms of this organism resulting in only a 21% reduction in surface coverage (Fig.…”

Section: Nitric Oxide Induces Dispersal Of Single-species Biofilms Ofmentioning

confidence: 76%

Nitric oxide‐mediated dispersal in single‐ and multi‐species biofilms of clinically and industrially relevant microorganisms

Barraud

Storey²,

Moore

et al. 2009

Microbial Biotechnology

265

276

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SummaryStrategies to induce biofilm dispersal are of interest due to their potential to prevent biofilm formation and biofilm‐related infections. Nitric oxide (NO), an important messenger molecule in biological systems, was previously identified as a signal for dispersal in biofilms of the model organism Pseudomonas aeruginosa. In the present study, the use of NO as an anti‐biofilm agent more broadly was assessed. Various NO donors, at concentrations estimated to generate NO levels in the picomolar and low nanomolar range, were tested on single‐species biofilms of relevant microorganisms and on multi‐species biofilms from water distribution and treatment systems. Nitric oxide‐induced dispersal was observed in all biofilms assessed, and the average reduction of total biofilm surface was 63%. Moreover, biofilms exposed to low doses of NO were more susceptible to antimicrobial treatments than untreated biofilms. For example, the efficacy of conventional chlorine treatments at removing multi‐species biofilms from water systems was increased by 20‐fold in biofilms treated with NO compared with untreated biofilms. These data suggest that combined treatments with NO may allow for novel and improved strategies to control biofilms and have widespread applications in many environmental, industrial and clinical settings.

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Section: Nitric Oxide Induces Dispersal Of Single-species Biofilms Ofmentioning

confidence: 76%

Nitric oxide‐mediated dispersal in single‐ and multi‐species biofilms of clinically and industrially relevant microorganisms

Barraud

Storey²,

Moore

et al. 2009

Microbial Biotechnology

265

276

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“…on May 7, 2018 http://science.sciencemag.org/ phylaxis (26). The acquisition of resistance to the quinolone nalidixic acid, mediated by various point mutations in the DNA gyrase gene, gyrA, was identified three times in Central and East Africa (Fig.…”

Section: Research | Reportmentioning

confidence: 99%

Genomic history of the seventh pandemic of cholera in Africa

Weill

Domman

Njamkepo

et al. 2017

Science

294

363

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The seventh cholera pandemic has heavily affected Africa, although the origin and continental spread of the disease remain undefined. We used genomic data from 1070 Vibrio cholerae O1 isolates, across 45 African countries and over a 49-year period, to show that past epidemics were attributable to a single expanded lineage. This lineage was introduced at least 11 times since 1970, into two main regions, West Africa and East/Southern Africa, causing epidemics that lasted up to 28 years. The last five introductions into Africa, all from Asia, involved multidrug-resistant sublineages that replaced antibiotic-susceptible sublineages after 2000. This phylogenetic framework describes the periodicity of lineage introduction and the stable routes of cholera spread, which should inform the rational design of control measures for cholera in Africa

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“…It is believed that subinhibitory concentrations of tetracycline have a positive selective effect on the dissemination and accumulation of tetracycline resistance genes in bacteria colonizing the gastrointestinal tract of mammals. This belief is based partly on molecular evidence, which indicates the tetracycline-induced transfer of mobile elements containing tetracycline resistance determinants (4), and partly on the observed increase in prevalence of various resistance determinants after therapeutic or prophylactic treatment with the drug (3,9). To investigate this effect further it is important to be able to determine the actual in situ bioavailable concentration of the antibiotic in the microhabitats hosting bacterial activity.…”

mentioning

confidence: 99%

In Vivo Detection and Quantification of Tetracycline by Use of a Whole-Cell Biosensor in the Rat Intestine

Bahl

Hansen

Licht

et al. 2004

Antimicrob Agents Chemother

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An Escherichia coli biosensor strain, harboring the plasmid pTGFP2, was introduced into the gastrointestinal tract of gnotobiotic rats that continuously received drinking water containing tetracycline. Plasmid pTGFP2 contains a transcriptional fusion between a green fluorescent protein (GFP) gene and a tetracyclineregulated promoter and was shown to produce a proportional GFP signal in response to exposure to various tetracycline concentrations when harbored by an E. coli strain. The plasmid was highly unstable in the host bacteria colonizing the intestinal system of the animals, and rapid plasmid loss was observed. Reintroduction of the E. coli MC4100/pTGFP2 strain into animals already colonized by the plasmid-free E. coli strain the day before euthanasia made it possible to extract and analyze the biosensors from intestinal samples. The induction of GFP in the biosensor cells extracted from the animals was estimated on a single-cell basis by use of flow cytometry, and the mean induction of GFP in the samples was compared to a standard curve prepared from known tetracycline concentrations. The results showed that the bioavailable tetracycline concentration within the bacterial growth habitat of the intestine was proportional to the concentration of tetracycline in drinking water but represented only approximately 0.4% of the intake concentration. This is a significant finding which will help to clarify antimicrobial therapy in the intestinal environment.Since their discovery in the 1940s, tetracyclines have been very useful antibiotics for the treatment of a whole range of bacterial infections in humans and animals and have also seen widespread use as growth promoters in animal husbandry (5). However, the emergence and characterization of some 36 different genes (17) conveying tetracycline or oxytetracycline resistance to the host bacteria by a number of different mechanisms have raised some concerns regarding the future usefulness of the tetracyclines. It is believed that subinhibitory concentrations of tetracycline have a positive selective effect on the dissemination and accumulation of tetracycline resistance genes in bacteria colonizing the gastrointestinal tract of mammals. This belief is based partly on molecular evidence, which indicates the tetracycline-induced transfer of mobile elements containing tetracycline resistance determinants (4), and partly on the observed increase in prevalence of various resistance determinants after therapeutic or prophylactic treatment with the drug (3, 9). To investigate this effect further it is important to be able to determine the actual in situ bioavailable concentration of the antibiotic in the microhabitats hosting bacterial activity. The traditional methods for measuring in situ antibiotic concentrations are based on an extraction of the compound from its natural matrix, followed by analysis by either chromatographic methods, such as high-pressure liquid chromatography (19), microbiological methods, such as bacterial inhibition assays (15), or in vitro biosensor ...

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Emergence and Rapid Spread of Tetracycline-Resistant Vibrio cholerae Strains, Madagascar

Cited by 27 publications

References 3 publications

Nitric oxide‐mediated dispersal in single‐ and multi‐species biofilms of clinically and industrially relevant microorganisms

Nitric oxide‐mediated dispersal in single‐ and multi‐species biofilms of clinically and industrially relevant microorganisms

Genomic history of the seventh pandemic of cholera in Africa

In Vivo Detection and Quantification of Tetracycline by Use of a Whole-Cell Biosensor in the Rat Intestine

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