Emergence of a carbapenem-resistant and colistin-heteroresistant Enterobacter cloacae clinical isolate in Japan

Uechi, Kohei; Tada, Tatsuya; Shimada, Kayo; Nakasone, Isamu; Kirikae, Teruo; Fujita, Jiro

doi:10.1016/j.jiac.2018.09.004

Cited by 17 publications

(19 citation statements)

References 19 publications

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“…Our investigation showed various amino acid substitutions: three amino acid substitution in phoP (L129I, F141L, H207Q), ve in phoQ (V102I, L133I, M298L, S448A, G464S), four in pmrA (A19G, S21A, N89T, L146Q) and four in pmrB (H132S, A172T, Q271V, R276Q). The amino acid substitutions observed in phoP, phoQ, and pmrA in this study corresponded with those reported by Uechi et al [25]. On the other hand, the amino acid substitutions observed in this study were different from those reported in another study by Nawfal Dagher et al [26].…”

Section: Cst-colistinsupporting

confidence: 84%

Colistin Resistance ㏌ Enterobacteriaceae Isolated from Arthropods in Gifu City, Japan

Odoi¹,

Yamamoto²,

Sugiyama³

et al. 2020

Preprint

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Background The emergence of antimicrobial-resistant (AMR) bacteria is an important concern for public and livestock health. Arthropods may serve as vectors that disseminate AMR bacteria across different environments. We examined the phenotype and genotype of antimicrobial resistance in Enterobacteriaceae collected from arthropods in Gifu city, Japan. Results A total of 186 Enterobacteriaceae from 94 arthropods were obtained and tested for antimicrobial susceptibility. All isolates were susceptible to the antimicrobial agents tested, except for colistin (39 isolates) and kanamycin (one isolate). The aph(3')-Ia gene and amino acid substitutions in the two-component system were responsible for the kanamycin and colistin resistance, respectively. Conclusion Overall Enterobacteriaceae isolated from the arthropods were susceptible to most of the antimicrobial agents. However, a high prevalence of colistin resistance was observed in the isolates from the arthropods. We suspect that this was a result of the production of antimicrobial peptides by the arthropods rather than selective pressure or exposure to colistin in the environment. Thus, arthropods maybe a potential reservoir of colistin resistant bacteria. These findings could be beneficial to public and livestock health management.

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Section: Cst-colistinsupporting

confidence: 84%

Colistin Resistance ㏌ Enterobacteriaceae Isolated from Arthropods in Gifu City, Japan

Odoi¹,

Yamamoto²,

Sugiyama³

et al. 2020

Preprint

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“…As reported by Mirelis, the total resistance rate of Enterobacteriaceae to colistin was 0.67% but the resistance rate of Enterobacter cloacae 4.2% was much higher than that of Escherichia coli and Klebsiella pneumoniae (23). The resistance mechanism of ECC strains to colistin can be mediated by the mcr-1 gene carried by the plasmid (15,24,25), and may also be related to the mutations of two-component system encoded by pmrAB and phoPQ (13). Recently, Zong et al found that a novel small transmembrane protein Ecr may mediate the heterogeneous resistance of ECC to colistin through the PhoPQ two-component system (16), but the distribution of ecr gene in ECC strains and its correlation with colistin resistance still need further study.…”

Section: Discussionmentioning

confidence: 72%

“…Furthermore, the overexpression of e ux pump AcrAB-TolC may also lead to the resistance of ECC strains to colistin (28). In recent years, carbapenems and colistin co-resistant ECCstrains have been found in multiple regions around the world (11)(12)(13)(29)(30)(31), which will further increase the di culty of treating MDR ECC infection. And some of the co-resistant ECC strains have the ability to spread drug resistance gene horizontally, which posing a huge threat to public health.…”

Section: Discussionmentioning

confidence: 99%

“…However, a number of recent studies have found that ECC strains carry carbapenemase genes while being resistant to colistin (11)(12)(13). Colistin is an old antibiotic which has been refocused in recent years.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the good activity against Gram-negative bacteria, it is considered to be one of the last-line antimicrobials for treatment of MDR Gram-negative bacteria (14). According to previous reports, ECC may acquire colistin resistance by plasmid-mediated gene mcr, small protein gene ecr or two-component systems phoPQ, pmrAB (13,15,16). The emergence of carbapenem-resistant and colistin-resistant ECC will undoubtedly pose a huge challenge to infection control.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of resistance mechanisms of Enterobacter cloacae Complex Co-resistant to Carbapenem and Colistin

Zhou

Liu

Fang

et al. 2020

Preprint

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Background: The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. The purpose of this study was to clarify the mechanism of the carbapenems and colistin co-resistance in Enterobacter cloacae Complex (ECC) strains.Methods: Nineteen ECC isolates co-resistant to carbapenems and colistin were collected from a regional medical center in China. Carbapenemase gene, extended-spectrum β-lactamase gene, AmpC cephalosporinase gene ampC, mcr series genes, and ecr gene were detected by PCR. Expression levels of outer membrane protein OmpC/OmpF and efflux pump protein AcrA/AcrB were investigated. And the structural modification of lipid A of 19 ECC strains was analyzed.Results: This study showed that the mechanisms of carbapenem resistance in this study are: 1. Generating carbapenemase (7 of 19); 2. The production of AmpC or ESBLs combined with decreased expression of out membrane protein (12 of 19). And the mechanism of colistin resistance is increaseing expression of acrA in the efflux pump AcrAB-TolC alone (5 of 19) or accompanied by a decrease of affinity between colistin and outer membrane caused by the modification of lipid A (14 of 19). Moreover, an ECC strain co-harboring plasmid-mediated mcr-4.3 and blaNDM-1 has been found.Conclusions: This study suggested that there is no overlap between the resistance mechanism of co-resistant ECC strains to carbapenem and colistin. However, the emergence of strain co-harboring plasmid-mediated resistance genes indicated that ECC is a potential carrier for the horizontal spread of carbapenems and colistin resistance.

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Molecular epidemiology and mechanisms of carbapenem and colistin resistance in Klebsiella and other Enterobacterales from treated wastewater in Croatia

Puljko,

Barišić,

Dekić Rozman

et al. 2024

Environment International

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Emergence of a carbapenem-resistant and colistin-heteroresistant Enterobacter cloacae clinical isolate in Japan

Cited by 17 publications

References 19 publications

Colistin Resistance ㏌ Enterobacteriaceae Isolated from Arthropods in Gifu City, Japan

Colistin Resistance ㏌ Enterobacteriaceae Isolated from Arthropods in Gifu City, Japan

Characterization of resistance mechanisms of Enterobacter cloacae Complex Co-resistant to Carbapenem and Colistin

Molecular epidemiology and mechanisms of carbapenem and colistin resistance in Klebsiella and other Enterobacterales from treated wastewater in Croatia

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