Emergence of ADC-5 Cephalosporinase in environmental Acinetobacter baumannii from a German tank milk with a novel Sequence Type

Sykes, Ellen M. E.; Mateo-Estrada, Valeria; Zhanel, George; Dettman, Jeremy R.; Chapados, Julie T.; Gerdis, Suzanne; Akineden, Ömer; Khan, Izhar U. H.; Castillo-Ramírez, Santiago; Kumar, Ayush

doi:10.1099/acmi.0.000485.v1

Cited by 3 publications

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“…Expression analysis was performed according to Sykes et al (12). In brief, overnight cultures of each strain were subcultured 1/100 in LB broth with shaking at 37ºC until mid exponential phase was not certified by peer review) is the author/funder.…”

Section: Rnd Efflux Expression Evaluationmentioning

confidence: 99%

“…In short, a One Health approach is required (11). By exploring the diversity of non-human Acinetobacter spp., further insight can be gathered into their evolution including novel STs, and antibiotic resistance genes, (12) as well as their potential to cause serious consequences for human health (13). In fact, emerging Acinetobacter spp, such as A. junii (14) and A. berenziniae (15) are being classified as One was not certified by peer review) is the author/funder.…”

Section: Introductionmentioning

confidence: 99%

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Lipidomics and colistin resistance in non-human isolates ofAcinetobacter seifertii

Sykes,

Mateo-Estrada,

Muzaleva

et al. 2024

Preprint

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Acinetobacter baumanniiis most well known for its role as a human pathogen and as a member of theAcinetobacter calcoaceticus-baumannii(ACB) complex. However, lesser characterised members of the ACB complex, have also been implicated in hospital-acquired infections. Once mainly considered opportunistic pathogens, manyA. baumanniiand non-baumanniistrains are being isolated from agricultural, water and food sources. The surveillance and tracking ofAcinetobacterspp. have been recently suggested to be part of the One Health consortium, to understand and prevent the spread of antimicrobial resistance. Here, we isolated fourAcinetobacterstrains from tank milk in Bogor, Indonesia and using ANI and dDDH techniques have identified them asAcinetobacter seifertii. MLST methods assigned theseA. seifertiistrains to a novel Sequence Types (ST), highlighting the diversity not only within the ACB complex but also in non-humanAcinetobacterspp. These fourA. seifertiistrains are colistin-resistant and while they do not harbour any known mechanism of colistin resistance, they do share amino acid substitutions in regulatory proteins, AdeS, PmrAB, H-NS, and the membrane associated proteins, LpxACD, MlaD, PldA, LpsB and EptA that may contribute to this phenotype. Furthermore, down-regulation of the RND efflux pump AdeAB, may also be a key factor in colistin resistance in these non-humanA. seifertiistrains. Lipidomics revealed an acyl-homoserine lactone (AHL) molecule, and lyso-phosphatidylethanolamine (lyso-PE) in significant abundance compared to colistin-sensitiveA. baumanniiATCC17978 revealing lipidomic differences between species. Finally, these four tank milkA. seifertiistrains are avirulent in an insect model of virulence. It is possible thatA. seifertiistrains are intrinsically resistant to colistin and require further study. By investigating these less understood Acinetobacter spp. from non-human sources, our study supports the One Health approach to combatting antibiotic resistance.

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Section: Rnd Efflux Expression Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipidomics and colistin resistance in non-human isolates ofAcinetobacter seifertii

Sykes,

Mateo-Estrada,

Muzaleva

et al. 2024

Preprint

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“…Another study, using several molecular typing strategies (MLST and the gene bla OXA-51 ), found this bacterium in water, milk, meat, cheese and animal samples [7]. A very recent study even found A. baumannii in a milk tank [11]. Clearly, isolates of this pathogen have been found in different types of environments [4,6,7,9,12].…”

Section: Introductionmentioning

confidence: 99%

“…A very recent study even found A. baumannii in a milk tank [11]. Clearly, isolates of this pathogen have been found in different types of environments [4, 6, 7, 9, 12].…”

Section: Introductionmentioning

confidence: 99%

Acinetobacter baumannii from grass: novel but non-resistant clones

et al. 2023

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Acinetobacter baumannii is one the most worrisome nosocomial pathogens, which has long been considered almost mainly as a hospital-associated bacterium. There have been some studies about animal and environmental isolates over the last decade. However, little effort has been made to determine if this pathogen dwells in the grass. Here, we aim to determine the evolutionary relationships and antibiotic resistance of clones of A. baumannii sampled from grass to the major human international clones and animal clones. Two hundred and forty genomes were considered in total from four different sources for this study. Our core and accessory genomic epidemiology analyses showed that grass isolates cluster in seven groups well differentiated from one another and from the major human and animal isolates. Furthermore, we found new sequence types under both multilocus sequence typing schemes: two under the Pasteur scheme and seven for the Oxford scheme. The grass isolates contained fewer antibiotic-resistance genes and were not resistant to the antibiotics tested. Our results demonstrate that these novel clones appear to have limited antibiotic resistance potential. Given our findings, we propose that genomic epidemiology and surveillance of A. baumannii should go beyond the hospital settings and consider the environment in an explicit One Health approach.

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“…Tracking of clonal spread also aids in tracking ARGs and VGs associated with particularly concerning clones. However, more and more often, A. baumannii from non-clinical sources (10)(11)(12)(13) are being interrogated because they too harbour ARGs and VGs. In fact, A. baumannii has been classified as a One Health problem (14), which encompasses all aspects of human, animal and environmental health.…”

Section: Introductionmentioning

confidence: 99%

Phylogenomic and phenotypic analyses highlight the diversity of antibiotic resistance and virulence in both human and non-humanAcinetobacter baumannii

Sykes,

Mateo-Estrada,

Engelberg

et al. 2023

Preprint

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Acinetobacter baumanniiis a Gram-negative opportunistic pathogen causing infections of the immunocompromised. With a high incidence of muti-drug resistance, carbapenem-resistantA. baumanniiis as a priority 1 pathogen designated by the WHO. The current literature has expertly characterized clinical isolates ofA. baumannii.As the challenge of these infections has recently been classified as a One Health issue, we set out to explore the diversity of isolates from human and non-clinical sources such as agricultural surface water, urban streams, various effluents from wastewater-treatment plants and tank milk; and, importantly, these isolates came from a wide geographic distribution. Phylogenomic analysis considering almost 200 isolates showed that our diverse set is well-differentiated from the main international clones ofA. baumannii. We discovered novel sequence types in both hospital and non-clinical settings, and five strains that overexpress the RND efflux pumpadeIJKwithout changes in susceptibility. Further, we detected ablaADC-79in a non-human isolate despite its sensitivity to all antibiotics. There was no significant differentiation between the virulence profiles of clinical and non-clinical isolates in theGalleria mellonellainsect model of virulence suggesting that virulence is neither dependent on geographic origin nor isolation source. Detection of antibiotic resistance and virulence genes in non-human strains suggests that these isolates may act as a genetic reservoir for clinical strains. This endorses the notion that in order to combat multi-drug resistant infection caused byA. baumannii,a One Health approach is required, and a deeper understanding of non-clinical strains must be achieved.ImportanceThe global crisis of antibiotic resistance is a silent one. More and more bacteria are becoming resistant to all antibiotics available for treatment, leaving no options remaining. This includesAcinetobacter baumannii.This Gram-negative opportunistic pathogen shows a high frequency of multi-drug resistance, and many strains are resistant to last-resort drugs carbapenem and colistin. Research has focused on strains of clinical origin, but there is a knowledge gap regarding virulence traits, particularly, howA. baumanniibecome the notorious pathogen of today. Antibiotic resistance and virulence genes have been detected in strains from animals, and environmental locations such as grass and soil. As such,A. baumanniiis a One Health concern which includes the health of humans, animals and the environment. Thus, in order to truly combat the antibiotic resistance crisis, we need to understand antibiotic resistance and virulence gene reservoirs of this pathogen under the One Health continuum.RepositoriesNCBI GenBank Accession numbers: Bioproject PRJNA819071, Biosamples SAMN26898552 - SAMN26898587.

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Emergence of ADC-5 Cephalosporinase in environmental Acinetobacter baumannii from a German tank milk with a novel Sequence Type

Cited by 3 publications

References 0 publications

Lipidomics and colistin resistance in non-human isolates ofAcinetobacter seifertii

Lipidomics and colistin resistance in non-human isolates ofAcinetobacter seifertii

Acinetobacter baumannii from grass: novel but non-resistant clones

Phylogenomic and phenotypic analyses highlight the diversity of antibiotic resistance and virulence in both human and non-humanAcinetobacter baumannii

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