Emergence of boscalid-resistant strains of Erysiphe necator in French vineyards

Cherrad, Semcheddine; Charnay, Aline; Hernandez, Catalina; Steva, H.; Belbahri, Lassaâd; Vacher, Sébastien

doi:10.1016/j.micres.2018.08.007

Cited by 34 publications

(30 citation statements)

References 27 publications

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“…Therefore, approaches targeting the pathogen through strict application of quarantine measures do not seem to enable eradication as promised by the early studies. Biological control is a promising approach that proved effective in the control of many plant pathogen and plant pest ecosystems (El Hassni et al, 2007; Dihazi et al, 2012; Abad et al, 2014; Alenezi et al, 2017; Mefteh et al, 2017, 2018; Cherrad et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium

Slama

Cherif‐Silini

Bouket³

et al. 2019

Front. Microbiol.

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113

144

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Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis, already responsible for destroying date production in other infected areas, mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, the additional benefits to agricultural ecosystems of using plant growth promoting rhizobacteria (PGPR) or endophytes are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization, meaning that it can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates, designated BFOA1, BFOA2, BFOA3 and BFOA4, that proved very active against F. oxysporum f. sp. albedinis. BFOA1–BFOA4 proved also active against 16 Fusarium isolates belonging to four species: F. oxysporum (with strains phytopathogenic of Olea europaea and tomato), F. solani (with different strains attacking O. europaea and potato), F. acuminatum (pathogenic on O. europaea) and F. chlamydosporum (phytopathogenic of O. europaea). BFOA1–BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens: Botrytis cinerea, Alternaria alternata, Phytophthora infestans, and Rhizoctonia bataticola. Isolates BFOA1–BFOA4 had the ability to grow at temperatures up to 35°C, pH range of 5–10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features, including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1–BFOA4 isolates indicated that they all belong to Bacillus halotolerans, which could therefore considered as a warden against Fusarium infection in plants. Comparative genomics allowed us to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses, enabling the description of diverse secondary metabolites including pulegone, 2-undecanone, and germacrene D, with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as an efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.

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Section: Introductionmentioning

confidence: 99%

Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium

Slama

Cherif‐Silini

Bouket³

et al. 2019

Front. Microbiol.

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113

144

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“…The results showed that 42.5% of the isolates had moderate levels of resistance to penthiopyrad (EC 50 = 335.1 – 787.6 mg/L), isopyrazam (EC 50 = 77.4–266.5 mg/L) and pyraziflumid (EC 50 = 9.2–31.2 mg/L); 44% of the isolates had high levels of resistance to penthiopyrad (EC 50 > 1000 mg/L), isopyrazam (EC 50 > 1070 mg/L) and pyraziflumid (EC 50 > 1000 mg/L); finally, only 1.5% showed high levels of resistance to isofatamid (EC 50 > 2400 mg/L) [ 161 ]. With respect to the grape powdery mildew, boscalid provided good control of E. necator until 2014, when a report described boscalid-resistant isolates collected from French vineyards that were able to grow at 30 and 100 mg/L of the formulated product [ 162 ] ( Table 2 ). Nevertheless, these isolates were inhibited with other SDHIs, such as fluxapyroxad and fluopyram [ 162 ].…”

Section: Current Fungicide Resistance Status In Powdery Mildew Funmentioning

confidence: 99%

“…With respect to the grape powdery mildew, boscalid provided good control of E. necator until 2014, when a report described boscalid-resistant isolates collected from French vineyards that were able to grow at 30 and 100 mg/L of the formulated product [ 162 ] ( Table 2 ). Nevertheless, these isolates were inhibited with other SDHIs, such as fluxapyroxad and fluopyram [ 162 ].…”

Section: Current Fungicide Resistance Status In Powdery Mildew Funmentioning

confidence: 99%

“…An additional mutation (A86V) in the subunit C was also correlated with high levels of resistance to isofetamid [ 161 ]. In E. necator , the amino acid changes H242R and H242Y in the SdhB subunit were correlated with resistance to boscalid [ 165 ] and with moderate levels of resistance to fluopyram and fluxapyroxad [ 162 ]. The amino acid change G169D in the SdhC subunit was also associated with a decrease in sensitivity to fluxapyroxad and fluopyram in this pathogen [ 162 ].…”

Section: Current Fungicide Resistance Status In Powdery Mildew Funmentioning

confidence: 99%

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Fungicide Resistance in Powdery Mildew Fungi

et al. 2020

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Powdery mildew fungi (Erysiphales) are among the most common and important plant fungal pathogens. These fungi are obligate biotrophic parasites that attack nearly 10,000 species of angiosperms, including major crops, such as cereals and grapes. Although cultural and biological practices may reduce the risk of infection by powdery mildew, they do not provide sufficient protection. Therefore, in practice, chemical control, including the use of fungicides from multiple chemical groups, is the most effective tool for managing powdery mildew. Unfortunately, the risk of resistance development is high because typical spray programs include multiple applications per season. In addition, some of the most economically destructive species of powdery mildew fungi are considered to be high-risk pathogens and are able to develop resistance to several chemical classes within a few years. This situation has decreased the efficacy of the major fungicide classes, such as sterol demethylation inhibitors, quinone outside inhibitors and succinate dehydrogenase inhibitors, that are employed against powdery mildews. In this review, we present cases of reduction in sensitivity, development of resistance and failure of control by fungicides that have been or are being used to manage powdery mildew. In addition, the molecular mechanisms underlying resistance to fungicides are also outlined. Finally, a number of recommendations are provided to decrease the probability of resistance development when fungicides are employed.

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“…In recent years, only three classes of fungicides, DMI (sterol C14 demethylation inhibitor in ergosterol biosynthesis), QoI (Qo site of complex III inhibitor in the respiratory chain), and SDHI (succinate dehydrogenase inhibitor in the respiratory chain), have accounted for approximately 60% of the global fungicide market 5 because of their high efficacy and the broad spectrum of these three chemical classes. Frequent usage of these three classes of fungicides has led to the development of resistance in crop pathogens, such as Zymoseptoria tritici, 6,7 Erysiphe necator, [8][9][10] Podosphaera xanthii, 11,12 Microdochium spp., 13,14 and Cercospora beticola. 15,16 As a result, agrochemical research and development seek new chemical classes of fungicides to respond to the needs of crop growers.…”

Section: Introductionmentioning

confidence: 99%

Pyridachlometyl has a novel anti‐tubulin mode of action which could be useful in anti‐resistance management

Matsuzaki

Watanabe

Harada

et al. 2019

Pest Management Science

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BACKGROUND Fungicide resistance is a growing problem affecting many crop pathogens owing to the low success rate in finding novel chemical classes of fungicides. Pyridachlometyl is a new fungicide that seems to belong to a new chemical class of tubulin polymerization promoters. RESULTS Pyridachlometyl exhibited potent antifungal activity against a broad range of fungal species belonging to the phyla Ascomycota and Basidiomycota. No cross‐resistance was observed with other fungicide classes, such as ergosterol biosynthesis inhibitors, respiratory inhibitors, or tubulin polymerization inhibitors in Zymoseptoria tritici. Pyridachlometyl‐resistant strains were obtainable by UV mutagenesis in Z. tritici and Penicillium digitatum. Mutations in tubulin‐coding genes were found in all laboratory mutants but the patterns of mutation were distinct from that of tubulin polymerization inhibitors, such as benzimidazole fungicides. CONCLUSION Pyridachlometyl is a promising new tool for disease control. However, strict resistance management strategies should be recommended for the practical use of pyridachlometyl. © 2019 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Emergence of boscalid-resistant strains of Erysiphe necator in French vineyards

Cited by 34 publications

References 27 publications

Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium

Screening for Fusarium Antagonistic Bacteria From Contrasting Niches Designated the Endophyte Bacillus halotolerans as Plant Warden Against Fusarium

Fungicide Resistance in Powdery Mildew Fungi

Pyridachlometyl has a novel anti‐tubulin mode of action which could be useful in anti‐resistance management

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