Emergence of Escherichia coli isolates producing NDM-1 carbapenemase from waterfowls in Hainan island, China

Rehman, Mujeeb Ur; Yang, Hong; Zhang, Shaqiu; Huang, Ya‐Hui; Zhou, Rui; Gong, Siyue; Feng, Qi; Chen, Shu‐Ling; Yang, Jing; Yang, Zhishuang; Abbas, Muhammad; Cui, Min; Wang, Mingshu; Jia, Renyong; Chen, Shun; Liu, Mafeng; Zhu, Dekang; Zhao, Xinxin; Wu, Ying; Yang, Qiao; Huang, Juan; Zhang, Ling; Liu, Yunya; Yu, Yanling; Tian, Bin; Cheng, Anchun

doi:10.1016/j.actatropica.2020.105485

Cited by 6 publications

(7 citation statements)

References 39 publications

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“…Either the global distribution of colistin-resistant E. coli or the rapid spread of the carbapenem-resistant Enterobacteriaceae have created enormous challenges for public health security. It is a more and more headache to solve the infection caused by MDR pathogens in human clinical treatment and animal husbandry ( Gao et al, 2016 ; Potter et al, 2016 ; Rehman et al, 2020 ; Zhang et al, 2021b , 2021c ). As a result, tigecycline has been recognized as the important antibiotic of last resort for the clinical treatment of certain bacterial infections.…”

Section: Discussion and Prospectsmentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

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With the large-scale use of antibiotics, antibiotic resistant bacteria (ARB) continue to rise, and antibiotic resistance genes (ARGs) are regarded as emerging environmental pollutants. The new tetracycline-class antibiotic, tigecycline is the last resort for treating multidrug-resistant (MDR) bacteria. Plasmid-mediated horizontal transfer enables the sharing of genetic information among different bacteria. The tigecycline resistance gene tet(X) threatens the efficacy of tigecycline, and the adjacent ISCR2 or IS26 are often detected upstream and downstream of the tet(X) gene, which may play a crucial driving role in the transmission of the tet(X) gene. Since the first discovery of the plasmid-mediated high-level tigecycline resistance gene tet(X4) in China in 2019, the tet(X) genes, especially tet(X4), have been reported within various reservoirs worldwide, such as ducks, geese, migratory birds, chickens, pigs, cattle, aquatic animals, agricultural field, meat, and humans. Further, our current researches also mentioned viruses as novel environmental reservoirs of antibiotic resistance, which will probably become a focus of studying the transmission of ARGs. Overall, this article mainly aims to discuss the current status of plasmid-mediated transmission of different tet(X) genes, in particular tet(X4), as environmental pollutants, which will risk to public health for the “One Health” concept.

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Section: Discussion and Prospectsmentioning

confidence: 99%

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Zhang

Wen²,

Wang³

et al. 2022

Front. Microbiol.

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“…The black presented STs of E. coli isolates from the fecal samples of giant pandas. The gray presented other major E. coli popular ST types found worldwide or in giant panda ( Ji et al, 2015 ; Yang et al, 2019 ; Rehman, 2020 ; Belas et al, 2022 ; Zhong et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…ST1193 is a new emerging virulent and an antimicrobial resistant clone among E. coli with a tendency to spread rapidly worldwide ( Zhong et al, 2022 ). ST10 and ST95 are globally disseminated pathogens of both humans and wildlife and linked with many resistance mechanisms ( Rehman, 2020 ). Fortunately, the STs found in our study had little association with the important pathogenic and drug-resistant STs mentioned in the above studies.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance, serogroups, virulence gene profiles and MLST of Escherichia coli from giant panda

Li,

Lv,

et al. 2024

Front. Microbiol.

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Escherichia coli is a major bacterial pathogen which causes diarrhea in the giant panda. This study investigated the biological characteristics of 100 E. coli strains isolated from fecal samples collected from 100 captive giant pandas of different age groups and sexes. A standard Kirby-Bauer disk diffusion antimicrobial susceptibility test was performed with the isolates and we then further evaluated the antibiotic resistance genes (ARGs) by high-throughput quantitative PCR. Additionally, we then analyzed O serogroups through a slide agglutination test, virulence genes and the multi-locus sequence typing (MLST) by PCR. Antimicrobial susceptibility testing demonstrated that the 100 E. coli strains were mainly resistant to ENR (68%), AM (56%), IPM (55%), AMX (54%) and CA (52%), but were susceptible to MEM and FOX. The resistance to TZP, AK, FEP, CAZ, AMS, AZM, AT and IPM was significantly related to age (p < 0.05); the resistance rate of E. coli isolated from female giant pandas to N was significantly higher than in males (p < 0.05). Forty-five different types of ARGs were found, which included a total of 2,258 ARGs, in the 100 E. coli isolates. The top 10 of detection rate of ARGs were: acrA-04, acrA-05, aacC, blaCTX-M-04, ampC-04, blaSHV-01, blaTEM, sul2, blaOXY, tetA-02. ARGs aac (6’)I1, blaCTX-M-03, tetD-02, blaSHV-02 and blaOXY were significantly related to age (p < 0.05), blaSHV-02, blaNDM and ampC-04 were related to sex (p < 0.05). Twelve different O serogroups from 32 E. coli isolates were distinguished, including O4, O8, O9, O15, O18, O20, O55, O88, O112, O157, O158, and O167. The most prevalent O serotype was O20, but O28, O45, O101, O149, and O152 were not detected. Fourteen different types of virulence genes were detected in the 100 E. coli isolates, of which papA (99%) were highly detected, while hlyA, elt and estA were not detected. MLST showed that 41 STs, which had one CCs and six groups with SLVs, in the 100 E. coli strains were identified, the main type was ST37. Our results advocate the need of strict biosecurity and surveillance programs in order to prevent the spread of pathogenic bacteria in the captive giant panda population.

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“…Para la detección en las cepas aisladas de E. coli, de genes asociados a carbapenemasas y genes de resistencia a la colistina se realizaron distintas PCRs. Para el estudio de resistencia frente a carbapenemasas se emplearon como genes diana los genes: bla NDM (Rehman et al, 2020) , bla KPC (Vera-Leiva et al, 2017) y bla IMP . Para el estudio de la resistencia frente a la colistina se empleó como gen diana el gen mcr-1 .…”

Section: Detección De Genes Asociados a Carbapenemasas Y De Resistenc...unclassified

“…La carbapenemasa NDM parece aportar niveles elevados de resistencia, al ser aisladas en varias bacterias multirresistentes. Se ha encontrado en las cepas que presentan el gen bla IMP que la sintesis de la carbapenemasa IMP se ve muy favorecida en el momento que comienza la maduración del biofilm estando relacionadas la hiperactivación del gen bla IMP con la colonización exitosa por parte de la bacteria Rehman et al, 2020) La transferencia horizontal de estos genes a través de los transposones, los integrones y la inserción de secuencias a partir de enterobacte-rias presentes en el suelo o en el agua a enterobacterias presentes en personas y/o animales juegan un papel fundamental en la prevalencia de las β-lactámasas (Wellington et al, 2013).…”

Section: Introductionunclassified

Resistencia Fenotípica Y Genotípica en Los Patotipos Aiec, Stec Y Eaec De E. Coli

Herrero¹,

Vergara²,

Hernández³

2022

An. Vet. Murcia

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Escherichia coli is a bacteria that is very present in our daily life, as a commensal or pathogenic organism. There are a large number of pathogenic strains capable of producing diseases, being of great importance for public health those strains transmitted through food, causing foodborne infections with great morbidity. In some cases, the strains that cause these outbreaks can be highly pathogenic, causing high mortality and very serious health implications for those affected. In this study, we focused on investigating the presence of strains transmitted through food of meat origin that belong to one of the 3 pathotypes investigated (STEC, AIEC and EAEC). On the other hand, we proposed to investigate the antibiotic resistance of E. coli strains isolated from meat foods. Our results showed the high phenotypic resistance of the great majority of E. coli strains isolated in this study, posing a serious problem for public health. This makes it necessary to maximize vigilance and control measures for these pathotypes throughout the food chain. It is also necessary to be cautious in the use of antibiotics, since their inappropriate use would lead to an increase in the therapeutic difficulty of infections, threatening to put an end to a century of medical advances. Escherichia coli es una bacteria que está muy presente en nuestro día a día, como comensal o como forma patógena. Hay gran cantidad de cepas patógenas capaces de producir enfermedades, siendo de gran importancia para la salud pública aquellas cepas vehiculadas a través de los alimentos, provocando toxiinfecciones alimentarias con gran morbilidad. En algunos casos, las cepas que provocan estos brotes puedes ser muy patógenas ocasionando una gran mortalidad y con repercusiones muy graves para los afectados. En este estudio nos centramos en investigar la presencia de cepas vehiculadas a través de los alimentos de origen cárnico que formen parte de alguno de los 3 patotipos investigados (STEC, AIEC y EAEC). Por otro lado, planteamos investigar la resistencia a los antibióticos que presentan las cepas de E. coli aisladas en los alimentos cárnicos. Nuestros resultados han evidenciado la elevada resistencia fenotípica que presentan la gran mayoría de las cepas de E. coli aisladas en este estudio, planteando un grave problema para la salud pública. Esto hace necesario extremar las medidas de vigilancia y control de estos patotipos a lo largo de la cadena alimentaria. También es necesario ser cautos en el empleo de antibióticos ya que si se hace un uso inadecuado de los mismos provocaría un aumento en la dificultad terapéutica de las infecciones, amenazando con acabar con un siglo de avances médicos.

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Emergence of Escherichia coli isolates producing NDM-1 carbapenemase from waterfowls in Hainan island, China

Cited by 6 publications

References 39 publications

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Dissemination and prevalence of plasmid-mediated high-level tigecycline resistance gene tet (X4)

Antimicrobial resistance, serogroups, virulence gene profiles and MLST of Escherichia coli from giant panda

Resistencia Fenotípica Y Genotípica en Los Patotipos Aiec, Stec Y Eaec De E. Coli

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