Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran

Azimi, Akram; Rezaei, Fatemeh; Yaseri, Mehdi; Jafari, Sara; Rahbar, Mohammad; Douraghi, Masoumeh

doi:10.1038/s41598-021-88977-z

Cited by 19 publications

(11 citation statements)

References 31 publications

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“…However, resistance development has never slowed, warranting suitable sensitizers. 111 Previous research has reported highlevel fluoroquinolone resistance of Shigella dysenteriae induced by a PMF-dependent efflux system. 112 Several fluoroquinolone adjuvants are found to influence ΔΨ or ΔpH.…”

Section: Pmf-modulating Compounds Resensitize Other Antibiotic Classesmentioning

confidence: 99%

Bacterial proton motive force as an unprecedented target to control antimicrobial resistance

Yang

Tong

Shi

et al. 2023

Medicinal Research Reviews

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Novel antibacterial therapies are urgently required to tackle the increasing number of multidrug‐resistant pathogens. Identification of new antimicrobial targets is critical to avoid possible cross‐resistance issues. Bacterial proton motive force (PMF), an energetic pathway located on the bacterial membrane, crucially regulates various biological possesses such as adenosine triphosphate synthesis, active transport of molecules, and rotation of bacterial flagella. Nevertheless, the potential of bacterial PMF as an antibacterial target remains largely unexplored. The PMF generally comprises electric potential (ΔΨ) and transmembrane proton gradient (ΔpH). In this review, we present an overview of bacterial PMF, including its functions and characterizations, highlighting the representative antimicrobial agents that specifically target either ΔΨ or ΔpH. At the same time, we also discuss the adjuvant potential of bacterial PMF‐targeting compounds. Lastly, we highlight the value of PMF disruptors in preventing the transmission of antibiotic resistance genes. These findings suggest that bacterial PMF represents an unprecedented target, providing a comprehensive approach to controlling antimicrobial resistance.

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Section: Pmf-modulating Compounds Resensitize Other Antibiotic Classesmentioning

confidence: 99%

Bacterial proton motive force as an unprecedented target to control antimicrobial resistance

Yang

Tong

Shi

et al. 2023

Medicinal Research Reviews

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“…strain I6 showed high similarity (identity ≥ 99.88%) with Stenotrophomonas maltophilia. This species is increasingly reported as an opportunistic and nosocomial pathogens (Alsuhaibani et al 2021 ) and it shows resistance to different antibiotics such as carbapenems and fluoroquinolone (Harmon et al 2019 ; Azimi et al 2021 ; Bostanghadiri et al 2021 ). Results of BLAST and RDP analyses were confirmed by the Bayesian and ML phylogenetic analyses inferred on the basis of the obtained 16S rRNA gene sequences (Fig.…”

Section: Resultsmentioning

confidence: 99%

Pathogenic potential of bacteria isolated from commercial biostimulants

et al. 2022

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Microbial-based products are a promising alternative to agrochemicals in sustainable agriculture. However, little is known about their impact on human health even if some of them, i.e., Bacillus and Paenibacillus species, have been increasingly implicated in different human diseases. In this study, 18 bacteria were isolated from 2 commercial biostimulants, and they were genotypically and phenotypically characterized to highlight specific virulence properties. Some isolated bacteria were identified as belonging to the genus Bacillus by BLAST and RDP analyses, a genus in-depth studied for plant growth-promoting ability. Moreover, 16S rRNA phylogenetic analysis showed that seven isolates grouped with Bacillus species while two and four clustered, respectively, with Neobacillus and Peribacillus. Unusually, bacterial strains belonging to Franconibacter and Stenotrophomonas were isolated from biostimulants. Although Bacillus species are generally considered nonpathogenic, most of the species have shown to swim, swarm, and produced biofilms, that can be related to bacterial virulence. The evaluation of toxins encoding genes revealed that five isolates had the potential ability to produce the enterotoxin T. In conclusion, the pathogenic potential of microorganisms included in commercial products should be deeply verified, in our opinion. The approach proposed in this study could help in this crucial step.

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“…However, in the last 10 years, an increase in the number of isolates resistant to this chemotherapeutic agent has been observed in various regions of the world [ 28 , 32 ]. The second commonly used group of drugs in the treatment of infections caused by S. maltophilia strains are the fluoroquinolones, mainly levofloxacin [ 28 , 33 , 34 ]. Despite the high cure rates of S. maltophilia infections with levofloxacin monotherapy, a trend towards the selection of resistance to this fluoroquinolone has been observed [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Molecular Characteristics of the MDR Efflux Pump Gene-Carrying Stenotrophomonas maltophilia Strains Isolated in Warsaw, Poland

Zając

Tyski

Laudy

2022

Biology

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An increase of nosocomial infections caused by Stenotrophomonas maltophilia strains has recently been observed all over the world. The isolation of these bacteria from the blood is of particular concern. In this study we performed the phenotypic and genotypic characterization of 94 S. maltophilia isolates, including isolates from patients hospitalized in a tertiary Warsaw hospital (n = 79) and from outpatients (n = 15). All isolates were found to be susceptible to trimethoprim-sulfamethoxazole and minocycline, while 44/94 isolates demonstrated a reduction in susceptibility to levofloxacin. A large genetic variation was observed among the isolates tested by pulsed-field gel electrophoresis. A clonal relationship with 100% similarity was observed between isolates within two sub-pulsotypes: the first included nine bloodstream isolates and the second involved six. Multilocus sequence typing showed two new sequence types (ST498 and ST499) deposited in public databases for molecular typing. Moreover, the presence of genes encoding ten different efflux pumps from the resistance-nodulation-division family and the ATP-binding cassette family was shown in the majority of the 94 isolates. The obtained knowledge about the prevalence of efflux pump genes in clinical S. maltophilia strains makes it possible to predict the scale of the risk of resistance emergence in strains as a result of gene overexpression.

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Emergence of fluoroquinolone resistance and possible mechanisms in clinical isolates of Stenotrophomonas maltophilia from Iran

Cited by 19 publications

References 31 publications

Bacterial proton motive force as an unprecedented target to control antimicrobial resistance

Bacterial proton motive force as an unprecedented target to control antimicrobial resistance

Pathogenic potential of bacteria isolated from commercial biostimulants

Phenotypic and Molecular Characteristics of the MDR Efflux Pump Gene-Carrying Stenotrophomonas maltophilia Strains Isolated in Warsaw, Poland

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